Cytokeratin expression in the epithelia of the adult human cochlea

Bauwens, L. J. J. M.; Groot, John C.M.J. de; Ramaekers, F.C.S.; Veldman, Jan E.; Huizing, Egbert H.

doi:10.1007/bf00176758

Cited by 10 publications

(7 citation statements)

References 12 publications

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“…Two separate antibodies to cytokeratins label the lumenal extensions of mature support cells in an increasing gradient from the cochlear base to its apex. A similar pattern of cytokeratin expression in support cells has been demonstrated in normal mammalian cochlea and vestibular organs (Raphael et al, 1987;Schrott et al, 1988;Anniko et al, 1989Anniko et al, , 1990Anniko and Arnold, 1990;Bauwens et al, 1991;Kuipers et al, 1991) but has not been reported previously in the chick inner ear. Cytokeratin labeling is pronounced in only a subset of support cells along the inferior, abneural edge of control basilar papillae.…”

Section: Cell-selective Markers In the Normal And Regenerating Cochlesupporting

confidence: 76%

“…We reacted whole mounts of the basilar papilla with antibodies to calmodulin, which have been shown to label hair cells selectively in the mature mammalian organ of Corti (Flock et al, 1986;Slepecky et al, 1988;Bauwens et al, 1991;Slepecky and Ulfendahl, 1993), and with the TUJ1 antibody, which labels class III ␤ tubulin in early differentiating neurons (Moody et al, 1989;Lee et al, 1990;Easter et al, 1993). In addition to examining the pattern of antibody labeling in the intact whole mounts, we embedded immunoreacted cochleae in plastic and sectioned them so that the cellular distribution of each antigen could be determined.…”

Section: Immunolabeling Of Hair Cells In Normal and Drugdamaged Cochlmentioning

confidence: 99%

“…We reacted whole mounts of the chick basilar papilla with antibodies to cytokeratin, which have been shown to label support cells selectively in the mature mammalian organ of Corti (Schrott et al, 1988;Anniko et al, 1989Anniko et al, , 1990Bauwens et al, 1991;Raphael and Altschuler, 1991). Two antibodies to the intermediate filament cytokeratin labeled support cells: AE1/AE3 (Boehringer Mannheim) and 8.13 (Sigma).…”

Section: Immunolabeling Of Support Cells In Normal and Drugdamaged Comentioning

confidence: 99%

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Hair Cell Differentiation in Chick Cochlear Epithelium after Aminoglycoside Toxicity:In VivoandIn VitroObservations

Leaño²,

et al. 1996

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Inner ear epithelia of mature birds regenerate hair cells after ototoxic or acoustic insult. The lack of markers that selectively label cells in regenerating epithelia and of culture systems composed primarily of progenitor cells has hampered the identification of cellular and molecular interactions that regulate hair cell regeneration. In control basilar papillae, we identified two markers that selectively label hair cells (calmodulin and TUJ1 ␤ tubulin antibodies) and one marker unique for support cells (cytokeratin antibodies). Examination of regenerating epithelia demonstrated that calmodulin and ␤ tubulin are also expressed in early differentiating hair cells, and cytokeratins are retained in proliferative support cells. Enzymatic and mechanical methods were used to isolate sensory epithelia from mature chick basilar papillae, and epithelia were cultured in different conditions. In control cultures, hair cells are morphologically stable for up to 6 d, because calmodulin immunoreactivity and phalloidin labeling of filamentous actin are retained. The addition of an ototoxic antibiotic to cultures, however, causes complete hair cell loss by 2 d in vitro and generates cultures composed of calmodulinnegative, cytokeratin-positive support cells. These cells are highly proliferative for the first 2-7 d after plating, but stop dividing by 9 d. Calmodulin-or TUJ1-positive cells reemerge in cultures treated with antibiotic for 5 d and maintained for an additional 5 d without antibiotic. A subset of calmodulinpositive cells was also labeled with BrdU when it was continuously present in cultures, suggesting that some cells generated in culture begin to differentiate into hair cells. Key words: hair cells; regeneration; chick; basilar papilla; cell culture; differentiationHair cells are sensory receptors for hearing, equilibrium, and motion detection. Some animals demonstrate the capacity to generate hair cells throughout their lifetime (Popper and Hoxter, 1984;Corwin, 1985; Jörgenson and Mathiessen, 1989;Roberson et al., 1992) or to initiate hair cell regeneration in the event of their loss (Corwin and Cotanche, 1988;Ryals and Rubel, 1988). The progenitors of hair cells seem to be a subset of support cells that reside adjacent to hair cells in the sensory epithelia (Girod et al., 1989;Balak et al., 1990;Raphael, 1992;Hashino and Salvi, 1993;Weisleder and Rubel, 1993;Stone and Cotanche, 1994;Tsue et al., 1994a;Roberson et al., 1996). Although mature mammals normally do not generate new hair cells, recent in vivo and in vitro studies have documented mitotic activity and immature-looking hair cells in mammalian vestibular epithelia after exposure to ototoxic drugs Warchol et al., 1993;Rubel et al., 1995), suggesting that hair cell regeneration in mammals may be inducible. The development of culture methods for mature cochlear and vestibular end organs has been initiated to identify molecules that regulate cell proliferation and differentiation in avian and mammalian hair cell epithelia. Co-culture experiments suggest that...

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Section: Cell-selective Markers In the Normal And Regenerating Cochlesupporting

confidence: 76%

Section: Immunolabeling Of Hair Cells In Normal and Drugdamaged Cochlmentioning

confidence: 99%

Section: Immunolabeling Of Support Cells In Normal and Drugdamaged Comentioning

confidence: 99%

See 1 more Smart Citation

Hair Cell Differentiation in Chick Cochlear Epithelium after Aminoglycoside Toxicity:In VivoandIn VitroObservations

Leaño²,

et al. 1996

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“…The same CK subtypes have recently been demonstrated in the adult human cochlea. 16 However, in the adult human endolymphatic duct and sac, CKs typical of complex epithelia were found, since in this part of the inner ear the additional presence of CKs 14 and 17 was demonstrated." thelium of the vestibular labyrinth.…”

Section: Discussionmentioning

confidence: 98%

Expression of Intermediate Filament Proteins in the Adult Human Vestibular Labyrinth

Bauwens¹,

Groot²,

Veldman³

et al. 1992

Ann Otol Rhinol Laryngol

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The immunohistochemical detection of intermediate filament proteins, cytoskeletal constituents that allow the characterization of tissues, was investigated in frozen sections of the chemically fixed, nondecalcified, adult human vestibular labyrinth. Cytokeratins (CKs) were detected in all epithelia (including the sensory epithelia), although substantial differences in the degree of staining between individual cells occurred. The expression of CKs 7, 8, 18, and 19 as detected with our subunit-specific monoclonal antibodies in the vestibular epithelia is typical of "simple" epithelia and is identical to the CK subtypes found in the human cochlea. Although immunostaining for CK 7 was very weak and was limited to certain vestibular wall cells, the other CKs demonstrated a pronounced and rather uniform distribution throughout the different epithelia. All epithelia (including the sensory epithelia) displayed expression of vimentin, thus demonstrating co-expression with CKs. Vimentin was also present in the subepithelial connective tissue fibroblasts and mesothelial lining of the vestibular labyrinth. Neurofilament proteins were detected in all neuronal structures. The intense staining for CKs in the maculae and cristae implies that these sensory organs are rigid structures, a finding that may possibly be of importance in the mechanoelectrical transduction process for the sense of equilibrium.

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“…Antibodies to keratins 8, 18, and 19 bind to supporting cells in humans [Anniko et al, 1990;Bauwens et al, 1991a] and rats as well as guinea pigs [Kuipers et al, 1991]. This report provides evidence that deployment of these keratins in guinea pig supporting cells is spatially precise and nonuniform.…”

Section: Keratin Filament Deploymentmentioning

confidence: 50%

Keratin filament deployment and cytoskeletal networking in a sensory epithelium that vibrates during hearing

Mogensen

Henderson

Mackie

et al. 1998

Cell Motil. Cytoskeleton

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Cytokeratin expression in the epithelia of the adult human cochlea

Cited by 10 publications

References 12 publications

Hair Cell Differentiation in Chick Cochlear Epithelium after Aminoglycoside Toxicity:In VivoandIn VitroObservations

Hair Cell Differentiation in Chick Cochlear Epithelium after Aminoglycoside Toxicity:In VivoandIn VitroObservations

Expression of Intermediate Filament Proteins in the Adult Human Vestibular Labyrinth

Keratin filament deployment and cytoskeletal networking in a sensory epithelium that vibrates during hearing

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