Cytokeratin expression and acetowhite change in cervical epithelium.

Maddox, P.; Szarewski, Anne; Dyson, J. E.; Cuzick, Jack

doi:10.1136/jcp.47.1.15

Cited by 31 publications

(26 citation statements)

References 5 publications

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“…We found that the late-versus early passage W12 cells cultured on organotypic rafts exhibited abnormal features resembling those seen in multistage progression of cervical carcinoma (30). The architecture of the raft tissue was analyzed by staining for keratin 19 (CK-19) and CK-10.…”

Section: Expression Analysis Of Jagged1 and Manic Fringe In Caski Celmentioning

confidence: 99%

Papillomavirus-Mediated Neoplastic Progression Is Associated with Reciprocal Changes in Jagged1 and Manic Fringe Expression Linked to Notch Activation

Veeraraghavalu

Pett

Kumar

et al. 2004

J Virol

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Section: Expression Analysis Of Jagged1 and Manic Fringe In Caski Celmentioning

confidence: 99%

Papillomavirus-Mediated Neoplastic Progression Is Associated with Reciprocal Changes in Jagged1 and Manic Fringe Expression Linked to Notch Activation

Veeraraghavalu

Pett

Kumar

et al. 2004

J Virol

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“…These calculations were done analogously to those for the cytoplasm described in Eqs. (9) to (11). For MR1 cells, the scattering efficiencies were about 8% higher for the masks covering the entire nucleus, indicating that stronger scattering was occurring from LysoSensor stained areas.…”

Section: Changes In Light Scattering When Acetic Acid Is Appliedmentioning

confidence: 93%

“…11 The strong increase in light scattering for MR1 cells which do not express cytokeratins demonstrates that cytokeratins are not critical for acetowhitening.…”

Section: Discussionmentioning

confidence: 99%

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Effects of acetic acid on light scattering from cells

Marina

2012

J. Biomed. Opt

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Abstract. Acetic acid has been used for decades as an aid for the detection of precancerous cervical lesions, and the use of acetic acid is being investigated in several other tissues. Nonetheless, the mechanism of acetowhitening is unclear. This work tests some of the hypotheses in the literature and measures changes in light scattering specific to the nucleus and the cytoplasm. Wide angle side scattering from both the nucleus and the cytoplasm increases with acetic application to tumorigenic cells, with the increase in nuclear scattering being greater. In one cell line, the changes in nuclear scattering are likely due to an increase in number or scattering efficiency of scattering centers smaller than the wavelength of excitation light. There are likely several cellular changes that cause acetowhitening and the cellular changes may differ with cell type. These results should lead to a better understanding of acetowhitening and potentially the development of adjunct techniques to improve the utility of acetic acid application. For the well-studied case of cervical tissue, acetowhitening has been shown to be sensitive, but not specific for oncogenic changes needing treatment.

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“…The mechanism causing the acetowhitening of the epithelium is not yet clear; it has been associated with a sexually transmitted agent [13], very likely a virus, that produces an increasing expression of proteins, such as cytokeratin 10 [18], and areas of high nuclear density. Our observations seem to suggest that high-risk HPV types might induce changes in the infected cells that produce a reaction with acetic acid.…”

mentioning

confidence: 99%

Molecular Evaluation of the Prevalence of Oncogenic Human Papillomavirus Genotypes in Cervical Acetowhite Lesions

et al. 2002

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The actual prevalence of cancer-related human papillomavirus (HPV) genotypes in cervical acetowhite lesions has not been established. In this work, the presence of oncogenic types of HPV in cervical acetowhite tissue was evaluated by molecular means. The presence of HPV DNA was determined in a group of women with and without cervical acetowhite lesions by a polymerase chain reaction (PCR) using the MY09/MY11 primers. The presence of 13 oncogenic HPV types was evaluated using the Hybrid Capture II test, and the prevalence of HPV type 16 (HPV16) was studied using an HPV16-specific PCR. HPV DNA was detected in 85.9% patients with acetowhite lesions; oncogenic HPV types were found in 83.7% of them; HPV16 was identified in 51.1% of the cases. HPV DNA was detected in 87.3% of the patients without acetowhite changes. Interestingly only 16.8% were infected by oncogenic genotypes and 2.5% were positive for the presence of HPV16. In conclusion, subclinical infection by oncogenic HPV genotypes is associated with the presence of acetowhite cervical tissue (p < 0.0005). Therefore, women showing acetowhite lesions might be at risk of developing cervical cancer.

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Cytokeratin expression and acetowhite change in cervical epithelium.

Cited by 31 publications

References 5 publications

Papillomavirus-Mediated Neoplastic Progression Is Associated with Reciprocal Changes in Jagged1 and Manic Fringe Expression Linked to Notch Activation

Papillomavirus-Mediated Neoplastic Progression Is Associated with Reciprocal Changes in Jagged1 and Manic Fringe Expression Linked to Notch Activation

Effects of acetic acid on light scattering from cells

Molecular Evaluation of the Prevalence of Oncogenic Human Papillomavirus Genotypes in Cervical Acetowhite Lesions

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