Cytokeratin and vimentin expression in breast cancer

Vora, Hemangini H.; Patel, Nupur; Rajvik, Kruti N.; Mehta, Shalvi V.; Brahmbhatt, Birwa V.; Shah, Manoj; Shukla, Shilin N.; Shah, Pankaj

doi:10.5301/jbm.2009.4965

Cited by 30 publications

(18 citation statements)

References 16 publications

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“…Clinical and pathological results of canine malignant mammary gland tumors in the present study. Numerous studies on vimentin demonstrated that EMT occurs in humans, as well as canines, and that vimentin expression is associated with tumor malignancy (14,15,17). However, unlike the previous studies, in the present study, no association was observed between vimentin expression and cancer stage, similar to the study by Terra et al (19).…”

Section: Discussioncontrasting

confidence: 56%

“…Some studies have demonstrated that the presence of vimentin biomarkers is linked to a poor prognosis in HBC (7,11,14). The study by Hemalatha et al (11) indicated that overexpression of vimentin is not associated with tumor grade and Ki-67, whereas Vora et al (14) concluded that the invasion potency is increased by the overexpression of vimentin and the loss of cytokeratin in breast cancer. However, Yamashita et al (7) evaluated the expression of vimentin in invasive ductal carcinoma of sporadic breast cancer and revealed that the overexpression of vimentin increased in the subtype of triple-negative breast cancer and was associated with invasiveness potency (8).…”

Section: Discussionmentioning

confidence: 99%

“…This mechanism occurs in breast cancer and results in the reduction or loss of cadherin adhesion molecules, loss of luminal cytokeratin, enhancement of basal cytokeratin, manifestation of different proteins, including α-smooth muscle actin, changes in the expression of matrix metalloproteinase genes and transforming growth factor-β (TGF-β) and increased expression of vimentin, thus facilitating migration and metastasis of malignant cells (14)(15)(16).…”

Section: Discussionmentioning

confidence: 99%

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Expression of vimentin filaments in canine malignant mammary gland tumors: A simulation of clinicopathological features of human breast cancer

et al. 2014

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Abstract. Canine malignant mammary gland tumors (CMMGTs) are the most common malignancies observed in females. Several biological similarities have been reported between CMMGTs and human breast cancer (HBC). The present study aimed to assess the correlation of vimentin filaments overexpression, as part of the process of epithelial-mesenchymal transition (EMT) and the clinicopathological characteristics in CMMGTs. The clinicopathological characteristics of 42 CMMGTs were collected. Paraffin-embedded blocks underwent immunohistochemistry staining, which was performed using vimentin (to assess the evolution of the EMT process), Ki-67 (for evaluation of tumor proliferation) and cluster of differentiation 34 (CD34) (for evaluation of angiogenesis) antibodies. The tumor stage, grade, vascular invasion, margin status, rate of expression of the vimentin filaments, microvessel density-CD34 and proliferation rate data were obtained. Finally, the association between the expression of the vimentin filaments and those parameters was resolved statistically. A significant association was shown between the overexpression of the vimentin filaments and tumor size (r=0.71, P=0.03), tumor grade (r=0.80, P=0.021), angiogenesis (r= 0.57, P= 0.043), proliferation coefficient (r= 0.06, P= 0.001) and vascular invasion (r=0.76, P=0.043). Vimentin overexpression did not statistically correlate with the tumor stage or the margin status. Similar to the findings of the present study, certain recent studies have indicated that vimentin filament expression in HBC and CMMGTs is associated with the severity of cancer. Thus, spontaneous canine mammary tumor models appear to be an appropriate animal model for breast cancer research, and the results of the present study could aid to reinforce the association.

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Section: Discussioncontrasting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Expression of vimentin filaments in canine malignant mammary gland tumors: A simulation of clinicopathological features of human breast cancer

et al. 2014

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“…In addition, the overexpression of vimentin, but not E-cadherin, was implicated in poorer disease-free survival in HCC patients. Vimentin expression has also been demonstrated to be correlated with the invasive phenotype in patients with gastric and breast cancer (14,15). Cancer cell invasion of the portal vein is a clinically defined characteristic of HCC.…”

Section: Discussionmentioning

confidence: 99%

Epithelial-mesenchymal transition expression profiles as a prognostic factor for disease-free survival in hepatocellular carcinoma: Clinical significance of transforming growth factor-β signaling

et al. 2012

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“…In cancer-related studies, vimentin has been found to be mainly involved in tumor mobility [22,23] . Vimentin has been shown to co-localize with α v β 3 -integrin (focal contacts) [24] .…”

Section: Effect Of Heparin On Brdu Incorporation and Ki67 Expression mentioning

confidence: 99%

Roles of vimentin and 14-3-3 zeta/delta in the inhibitory effects of heparin on PC-3M cell proliferation and B16-F10-luc-G5 cells metastasis

et al. 2012

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Aim: To investigate the inhibitory effects of heparin on PC-3M cells proliferation in vitro and B16-F10-luc-G5 cells metastasis in Balb/c nude mice and identify the protein expression patterns to elucidate the action mechanism of heparin. Methods: Human prostate cancer PC-3M cells were incubated with heparin 0.5 to 125 μg/mL for 24 h. The proliferation of PC-3M cells was assessed by MTS assay. BrdU incoporation and Ki67 expression were detected using a high content screening (HCS) assay. The cell cycle and apoptosis of PC-3M cells were tested by flow cytometry. B16-F10-luc-G5 cardinoma cells were injected into the lateral tail vein of 6-week old male Balb/c nude mice and heparin 30 mg/kg was administered iv 30 min before and 24 h after injection. The metasis of B16-F10-luc-G5 cells was detected by bioluminescence assay. Activated partial thromboplastin time (APTT) and hemorheological parameters were measured on d 14 after injection of B16-F10-luc-G5 carcinoma cells in Balb/c mice. The global protein changes in PC-3M cells and frozen lung tissues from mice burdened with B16-F10-luc-G5 cells were determined by 2-dimensional gel electrophoresis and image analysis. The protein expression of vimentin and 14-3-3 zeta/delta was measured by Western blot. The mRNA transcription of vimentin, transforming growth factor (TGF)-β, E-cadherin, and α v -integrin was measured by RT-PCR. Results: Heparin 25 and 125 μg/mL significantly inhibited the proliferation, arrested the cells in G 1 phase, and suppressed BrdU incorporation and Ki67 expression in PC-3M cells compared with the model group. But it had no significant effect on apoptosis of PC-3M cells. Heparin 30 mg/kg markedly inhibits the metastasis of B16-F10-luc-G5 cells on day 8. Additionally, heparin administration maintained relatively normal red blood hematocrit but had no influence on APTT in nude mice burdened with B16-F10-luc-G5 cells. Thirty of down-regulated protein spots were identified after heparin treatment, many of which are related to tumor development, extracellular signaling, energy metabolism, and cellular proliferation. Vimentin and 14-3-3 zeta/delta were identified in common in PC-3M cells and the lungs of mice bearing B16-F10-luc-G5 carcinoma cells. Heparin 25 and 125 μg/mL decreased the protein expression of vimentin and 14-3-3 zeta/delta and the mRNA expression of α v -integrin. Heparin 125 μg/mL decreased vimentin and E-cadherin mRNA transcription while increased TGF-β mRNA transcription in the PC-3M cells, but the differences were not significant. Transfection of vimentin-targeted siRNA for 48 h significantly decreased the BrdU incoporation and Ki67 expression in PC-3M cells. Conclusion: Heparin inhibited PC-3M cell proliferation in vitro and B16-F10-luc-G5 cells metastasis in nude mice by inhibition of vimentin, 14-3-3 zeta/delta, and α v -integrin expression.

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Cytokeratin and vimentin expression in breast cancer

Abstract: Loss of cytokeratin and gain of vimentin expression are indicators of biologically aggressive breast carcinoma.

Cited by 30 publications

References 16 publications

Expression of vimentin filaments in canine malignant mammary gland tumors: A simulation of clinicopathological features of human breast cancer

Expression of vimentin filaments in canine malignant mammary gland tumors: A simulation of clinicopathological features of human breast cancer

Epithelial-mesenchymal transition expression profiles as a prognostic factor for disease-free survival in hepatocellular carcinoma: Clinical significance of transforming growth factor-β signaling

Roles of vimentin and 14-3-3 zeta/delta in the inhibitory effects of heparin on PC-3M cell proliferation and B16-F10-luc-G5 cells metastasis

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