Cytokeratin and Vimentin Expression in Breast Cancer

Vora, Hemangini H.; Patel, Nupur; Rajvik, Kruti N.; Mehta, Shalvi V.; Brahmbhatt, Birwa V.; Shah, Manoj; Shukla, Shilin N.; Shah, Pankaj

doi:10.1177/172460080902400106

Cited by 45 publications

(33 citation statements)

References 16 publications

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“…These results indicate that EMT-like phenomenon occurs in canine tissues as with human breast cancer [6, 21]. However, these two changes were not always observed simultaneously in the same tissue, rather, weak correlation was observed between the loss of epithelial markers and gain of mesenchymal markers (Table 4).…”

Section: Discussionmentioning

confidence: 92%

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The Relationship between Clinicopathological Features and Expression of Epithelial and Mesenchymal Markers in Spontaneous Canine Mammary Gland Tumors

Yoshida

Choisunirachon

et al. 2014

The Journal of Veterinary Medical Science

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It is known that epithelial mesenchymal transition (EMT) contributes to the acquisition of malignant property in human cancers. However, the role of EMT in canine tumors remains to be elucidated. To evaluate the correlation between expression levels of protein markers involved in EMT and clinicopathological characteristics in canine mammary gland tumors, immunohistochemistry using antibodies against ZO-1, E-cadherin, vimentin, N-cadherin and fibronectin was performed on 119 clinical tissue samples. Consequently, loss of ZO-1 and E-cadherin, and gain of vimentin and N-cadherin were more frequently observed in malignant tumors than in benign tumors. However, there was no correlation among expression of these molecules. Univariate and multivariate analysis identified that loss of E-cadherin independently had a low one-year survival rate (adjusted odds ratio: 2.3, P=0.02). These results suggested that EMT might relate to acquisition of malignancy, and additionally, E-cadherin was strongly correlated with malignant behavior in canine mammary gland tumors.

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Section: Discussionmentioning

confidence: 92%

“…Several studies have been made on the relationship between EMT and malignancy of human cancers by investigating the expressions of these markers. Vora et al reported that loss of cytokeratin and gain of vimentin expression predicted biological aggressiveness including recurrence and metastasis in human breast cancer [21]. …”

mentioning

confidence: 99%

The Relationship between Clinicopathological Features and Expression of Epithelial and Mesenchymal Markers in Spontaneous Canine Mammary Gland Tumors

Yoshida

Choisunirachon

et al. 2014

The Journal of Veterinary Medical Science

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“…Interestingly, polygonal cells in anaplastic areas and TiHo-0906 cells co-expressed CKs and mesenchymal markers. In human breast cancer, this is associated with poor prognosis 27,55,56 , in canine and human cellular models, with malignancy 38,55 .…”

Section: Discussionmentioning

confidence: 99%

“…The High-mobility group AT-hook 2 protein (HMGA2) activates a range of EMT transcription factors implicated in the repression of epithelial genes, and mesenchymal genes up-regulation 24,25 . Therefore, EMT-derived cells are usually characterized by a higher HMGA2 expression 9,26 , loss or reduced expression of E-cadherin (E-cad), up-regulation of vimentin (Vim) 7,9 , and co-expression of epithelial markers (cytokeratins [CKs]) and mesenchymal markers (calponin [CALP], smooth muscle actin [SMA], and Vim) 7,27 . Other markers like CD44 participate in the downregulation of E-cad 12,28 .…”

Section: Introductionmentioning

confidence: 99%

TiHo-0906: a new feline mammary cancer cell line with molecular, morphological, and immunocytological characteristics of epithelial to mesenchymal transition

Granados-Soler

Junginger

Hewicker‐Trautwein

et al. 2018

Sci Rep

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Feline mammary carcinomas (FMCs) with anaplastic and malignant spindle cells histologically resemble the human metaplastic breast carcinoma (hMBC), spindle-cell subtype. hMBCs display epithelial-to-mesenchymal transition (EMT) characteristics. Herein we report the establishment and characterization of a cell line (TiHoCMglAdcar0906; TiHo-0906) exhibiting EMT-like properties derived from an FMC with anaplastic and malignant spindle cells. Copy-number variations (CNVs) by next-generation sequencing and immunohistochemical characteristics of the cell line and the tumour were compared. The absolute qPCR expression of EMT-related markers HMGA2 and CD44 was determined. The growth, migration, and sensitivity to doxorubicin were assessed. TiHo-0906 CNVs affect several genomic regions harbouring known EMT-, breast cancer-, and hMBCs-associated genes as AKT1, GATA3, CCND2, CDK4, ZEB1, KRAS, HMGA2, ESRP1, MTDH, YWHAZ, and MYC. Most of them were located in amplified regions of feline chromosomes (FCAs) B4 and F2. TiHo-0906 cells displayed an epithelial/mesenchymal phenotype, and high HMGA2 and CD44 expression. Growth and migration remained comparable during subculturing. Low-passaged cells were two-fold more resistant to doxorubicin than high-passaged cells (IC50: 99.97 nM, and 41.22 nM, respectively). The TiHo-0906 cell line was derived from a poorly differentiated cellular subpopulation of the tumour consistently displaying EMT traits. The cell line presents excellent opportunities for studying EMT on FMCs.

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“…The immunohistochemical staining results from each group and from each view were analyzed. The staining intensity was scored according to the following scale [15,16]: colorless staining was 0 points, light yellow was 1 point, brownish yellow was 2 points, and tan was 3 points. Positive cells were counted under the same lens using the following scale: no positive cells was 0 points, 1-10% positive cells was 1 point, 11-50% was 2 points, 51-75% was 3 points, and >75% was 4 points.…”

Section: Specimensmentioning

confidence: 99%

The expression of PEBP4 protein in lung squamous cell carcinoma

Chen

et al. 2011

Tumor Biol.

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The phosphatidylethanolamine-binding protein 4 (PEBP4) is a member of the PEBP family. It not only plays a role in the inhibition of the MAPK signaling pathway but also is involved in the inhibition of the JNK pathway that promotes the activation of AKT. Recent research has also shown that overexpression of PEBP4 was related to the development, invasion, and metastasis of a variety of tumors. This study aimed to investigate the correlation between PEBP4 protein expression in lung squamous cell carcinoma tissue and the clinical pathology of lung squamous cell carcinoma. Immunohistochemistry was used to detect PEBP4 expression in lung squamous cell carcinoma tissue and adjacent normal tissue from 61 patients. Western blotting was used to detect changes in the expression of PEBP4 protein between lung squamous cell carcinoma tissue and adjacent normal tissues. The correlation of PEBP4 expression and the occurrence, development, and clinical pathology of lung squamous cell carcinoma was analyzed. Of 61 patients, four patients were PEBP4 negative (-; 6.6%) and 57 patients were positive (+ to +++; 93.4%). Of those positive for PEBP4 expression, 7 patients were weakly positive (+; 11.5%), 21 patients were positive (++; 34.4%), and 29 patients were strongly positive (+++; 47.5%). PEBP4 protein was more highly expressed in lung squamous cell carcinoma tissue than in the adjacent normal lung tissue (p < 0.05). In PEBP4-positive patients, PEBP4 protein expression was significantly greater in those with lymph node metastases than in those without (p < 0.05). PEBP4 expression was significantly lower in patients at early (I and II) stages than in patients at advanced (III and IV) stages (p < 0.05). In less differentiated lung squamous cell carcinomas, PEBP4 protein expression was greater (p < 0.05); however, this was unrelated to the gender, age, or tumor size of the patient (p > 0.05). PEBP4 protein overexpression was associated with the occurrence, invasion, and metastasis of lung squamous cell carcinoma.

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Cytokeratin and Vimentin Expression in Breast Cancer

Abstract: Loss of cytokeratin and gain of vimentin expression are indicators of biologically aggressive breast carcinoma.

Cited by 45 publications

References 16 publications

The Relationship between Clinicopathological Features and Expression of Epithelial and Mesenchymal Markers in Spontaneous Canine Mammary Gland Tumors

The Relationship between Clinicopathological Features and Expression of Epithelial and Mesenchymal Markers in Spontaneous Canine Mammary Gland Tumors

TiHo-0906: a new feline mammary cancer cell line with molecular, morphological, and immunocytological characteristics of epithelial to mesenchymal transition

The expression of PEBP4 protein in lung squamous cell carcinoma

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