Cytokeratin and protein expression patterns in squamous cell carcinoma of the oral cavity provide evidence for two distinct pathogenetic pathways

Frohwitter, Gesche; Buerger, Horst; Diest, Paul J. van; Korsching, Eberhard; Kleinheinz, Johannes; Fillies, Thomas

doi:10.3892/ol.2016.4588

Cited by 38 publications

(30 citation statements)

References 44 publications

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“…In the report of Schwerer et al, cytokeratins CK10 and CK13 typical for squamous differentiation and cytokeratin 20 were negative in all WT specimens and its metaplastic variant . Also, smooth muscle actin (SMA) expressed in myoepithelial cells was not detected in WTs.…”

Section: Wt Immunophenotypementioning

confidence: 94%

“…However, in another study, CK10 immunoreactivity was found in five of eight WTs . Immunoreactivity for CK 5/14 and 17, typical of regenerative cells, was restricted to basal cells in WT, and for basal and surface cells in metaplastic WT …”

Section: Wt Immunophenotypementioning

confidence: 97%

“…Immunodiagnostics is not essential for the diagnosis of WT, except in the case of the metaplastic variant . The epithelial component of WT and its metaplastic variant are immunopositive for cytokeratin 7, 8, 18 and 19 . Sava et al reported that CK19 was positive in both luminal and basal epithelial cells of WT and that p63 nuclei were positive only in the basal cells.…”

Section: Wt Immunophenotypementioning

confidence: 99%

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Cytopathology and diagnostics of Warthin's tumour

Sučić

Ljubić²,

Perković³

et al. 2020

Cytopathology

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Warthin's tumour (WT) is a benign epithelial salivary tumour, one type of salivary adenoma. Histologically, WT is structured of two components, epithelial tissue that often lines cystic formations and lymphoid tissue in the tumour stroma. FNA is a reliable diagnostic approach in the diagnosis of salivary gland lesions allowing a highly accurate categorization of benign tumour‐like lesions, benign tumours and malignant tumours. In the proposed Milan reporting system of salivary gland lesions, WT is categorized in the IVA group of benign neoplasms. Accurate cytological diagnosis is straightforward when three characteristic components are present: oncocytes, either isolated or associated in clusters, lymphocytes and lymphoid cells and often an inflammatory/necrotic‐like substance. Also, specific features of scintigraphy and radiological imaging contribute to the diagnosis of WT. WT is categorized according to Seifert G. et al in 4 types, depending on the proportions of the epithelial component and lymphoid stroma. Differential cytopathological and pathohistological diagnosis include other salivary gland lesions with lymphoid, oncocytic epithelial and cystic components. In some cases, such as the metaplastic WT variant, there are additional cytopathological and histological diagnostic difficulties. Moreover, bilateral, multicentric or multiple and infrequently seen extra‐salivary localizations of WT are associated with further cytopathological diagnostic difficulties. Also, a rare possibility of malignant transformation of the epithelial or lymphoid component of WT as well as possible association with other primary tumours remains a challenge in accurate cytopathological and histological diagnosis of WT.

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Section: Wt Immunophenotypementioning

confidence: 94%

Section: Wt Immunophenotypementioning

confidence: 97%

Section: Wt Immunophenotypementioning

confidence: 99%

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Cytopathology and diagnostics of Warthin's tumour

Sučić

Ljubić²,

Perković³

et al. 2020

Cytopathology

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show abstract

“…The cytokeratins are considered to be the largest intermediate filaments of squamous epithelium and are critically important for the stabilization, shape, intracellular communication and transport within the cell. Therefore, cytokeratin expression is of considerable importance in tumor progression (Frohwitter et al, 2016). Based on cytokeratin immunostaining, all of the tumor parenchyma and squamous mucosal epithelial cells were identified as having positive cytoplasmic staining, and the tumor was confirmed to be of epithelial origin.…”

Section: Discussionmentioning

confidence: 98%

Perivulvar squamous cell carcinoma in a cow

Oral

Beytut

Kuru

et al. 2019

J Hellenic Vet Med Soc

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We present a case of perivulvar squamous cell carcinoma in an 8-year-old crossbred Simmental cow. A tumoral mass, of considerably large volume localized in the perivulvar region and growing at a slow pace, was detected in the animal. The mass, subsequently identified histopathologically and immunohistochemically as squamous cell carcinoma, was surgically excised from the perivulvar region using intrathecal anesthesia.

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“…Moreover recent reports suggest that interactions between keratins and other proteins modulate signaling cascades that regulate cell migration, invasion, and metastasis. According to this suggestion several cytokeratins were found differently expressed in malignant epithelial cells, their specific expression pattern frequently correlated with the prognosis and metastatic potential of certain tumors, like in breast cancer and tumors of the oral cavity [ 24 – 25 ]. KRT1 is coexpressed with KRT10 in the suprabasal layer of the epidermis, elevated expression of the KRT1/KRT10 complex indicate increased terminal differentiation of keratinocytes.…”

Section: Discussionmentioning

confidence: 99%

Analysis of KRT1, KRT10, KRT19, TP53 and MMP9 expression in pediatric and adult cholesteatoma

et al. 2018

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Cholesteatoma is an epidermal cyst with still unknown pathomechanism. The aim of the current study was to investigate molecular differences in the background of the hyperproliferative property and aggressive behavior typical of the cholesteatoma epithelium. The expression of three cytokeratin genes (KRT1, KRT10 and KRT19), the matrix metalloproteinase 9 gene (MMP9) and the tumor suppressor TP53 gene was measured by qRT-PCR in surgical samples of pediatric and adult cholesteatoma cases and their expression level was compared to that of normal skin samples from the retroauricular region of control individuals. Cholesteatoma samples were stratified according to the age of onset and recurrence for more detailed analysis. Our results showed identical expression pattern for KRT1 and KRT10, their expression was higher in pediatric cases than in adults, especially in pediatric recurrent samples. The expression level of KRT19 was inversely proportional to that of KRT1/KRT10, it was lower in the more invasive recurrent cases both in our pediatric and adult groups. As it was expected from the bone destructive behavior of cholesteatoma, a significantly elevated expression of MMP9 was measured in cholesteatoma samples, the highest level was found in adult recurrent cases. Low expression levels characterize the TP53 gene without significant differences in our samples. These findings demonstrate that cytokeratin expression distinguishes between pediatric/adult, nonrecurrent/recurrent cases, suggesting that distinct differentiation state and cell division potential characterize these cholesteatoma cases. KRT19 with a tumor suppressor potential might restrict the recurrence of cholesteatoma. The differences observed in gene expression profiles between cholesteatoma and control samples support the notion that cholesteatoma is a cystic lesion with tumor-like behavior because it is characterized by invasive, destructive growth and high tendency for recurrence.

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Cytokeratin and protein expression patterns in squamous cell carcinoma of the oral cavity provide evidence for two distinct pathogenetic pathways

Cited by 38 publications

References 44 publications

Cytopathology and diagnostics of Warthin's tumour

Cytopathology and diagnostics of Warthin's tumour

Perivulvar squamous cell carcinoma in a cow

Analysis of KRT1, KRT10, KRT19, TP53 and MMP9 expression in pediatric and adult cholesteatoma

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