Cytohesin-1 regulates human blood neutrophil adhesion to endothelial cells through β2 integrin activation

Azreq, Mohammed-Amine El; Bourgoin, Sylvain G.

doi:10.1016/j.molimm.2011.03.018

Cited by 21 publications

(24 citation statements)

References 42 publications

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“…HMVECs express both the BLT1 and the BLT2 receptors on their surface and increase ICAM-1 and release chemokines in response to proinflammatory stimuli resulting in PMN adhesion. 20,[24][25][26][27][28] Previous data demonstrated that leukotriene B4-mediated HMVEC activation occurred via the BLT2 receptor in congruence with the presented data, and the BLT2 receptor has been implicated in other pulmonary diseases and angiogenesis. 20,[29][30][31] Furthermore, the concentration of LPS employed as a positive control may also seem excessive; however, it is based on the human response to injected LPS in which concentrations of 10 ng/mL injected induced hypotensive shock and a concentration of 500 ng/mL induced both shock and signs of ALI and not just fevers and changes in cytokine profiles.…”

Section: Discussionsupporting

confidence: 89%

Supernatants and lipids from stored red blood cells activate pulmonary microvascular endothelium through the BLT2 receptor and protein kinase C activation

et al. 2017

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Background Although transfusion is a life-saving intervention, it may be associated with significant morbidity in injured patients. We hypothesize that stored red blood cells induce pro-inflammatory activation of human pulmonary microvascular endothelium (HMVECs) resulting in neutrophil (PMN) adhesion and predisposition to acute lung injury (ALI). Methods Ten units of red blood cells (RBCs) were collected and 50% (by weight) were leukoreduced (LR-RBCs) and the remainder was unmodified and stored in additive solution-5 (AS-5). Another 10 units of RBCs were collected, leukoreduced, and stored in AS-3. HMVECs were incubated with [10–40%]FINAL of the supernatants day (D)1-D42 of storage, lipid extracts and purified lipids. Endothelial surface expression of ICAM-1, IL-8 release, and PMN adhesion to HMVECs were measured. HMVEC signaling via the BLT2 receptor was evaluated. Supernatants and lipids were also employed as the first event in a two-event model of ALI. Results The supernatants [10–40%]FINAL from D21 LR-RBCs and D42 RBCs and LR-RBCs and the lipids from D42 stored in AS-5 induced increased ICAM-1 surface expression on endothelium, IL-8 release, and PMN adhesion. In addition, the supernatants [20–40%]FINAL from D21 and D42 RBCs in AS-5 also increased endothelial surface expression of ICAM-1. D42 supernatants and lipids also caused co-precipitation of β-arrestin-1 with BLT2, PKCβI, and PKCδand served as the first event in a two-event rodent model of ALI. In conclusion, lipids that accumulate during RBC storage activate endothelium and predispose to ALI, which may explain some of the adverse events associated with the transfusion of critically injured patients.

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Section: Discussionsupporting

confidence: 89%

Supernatants and lipids from stored red blood cells activate pulmonary microvascular endothelium through the BLT2 receptor and protein kinase C activation

et al. 2017

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“…Although the previous studies of CYTH1 in mature blood cell had mainly shown a functional association with integrin b2, 27 several independent findings in our study suggest that integrin b1 functions are also directly regulated by CYTH1, at least in HSPCs. First, we show in vitro that the adhesive interactions with the integrin b1 ligand RN are severely impaired by CYTH1 knockdown.…”

Section: Discussioncontrasting

confidence: 68%

“…19 Further studies have extended the role of cytohesins in regulating integrin functions to other cell types such as neutrophils and dendritic cells, and the precise adhesion-mediating effect of CYTH1 has been found to be highly context and cell-type dependent. [26][27][28] Our findings now extend the role of CYTH1 to HSPCs in addition to mature blood cells.…”

Section: Discussionsupporting

confidence: 64%

Cytohesin 1 regulates homing and engraftment of human hematopoietic stem and progenitor cells

Rak

Foster

Potrzebowska³

et al. 2017

Blood

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“…CYTH1 regulates adhesion and transendothelial migration of monocytes, T lymphocytes, and dentritic cells; it is also a key regulator of neutrophil adhesion to endothelial cells and to components of the extracellular matrix. 47,48 RPS6KB2 plays an important role in neutrophilic differentiation and neutrophilic proliferation in human promyelocytic leukemia (HL-60) cells. Hence, methylation changes at pertinent CpG sites within these 2 genes may affect immune function.…”

Section: Discussionmentioning

confidence: 99%

Differential DNA methylation and PM_2.5 species in a 450K epigenome-wide association study

et al. 2017

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Although there is growing evidence that exposure to ambient particulate matter is associated with global DNA methylation and gene-specific methylation, little is known regarding epigenome-wide changes in DNA methylation in relation to particles and, especially, particle components. Using the Illumina Infinium HumanMethylation450 BeadChip, we examined the relationship between one-year moving averages of PM 2.5 species (Al, Ca, Cu, Fe, K, Na, Ni, S, Si, V, and Zn) and DNA methylation at 484,613 CpG probes in a longitudinal cohort that included 646 subjects. Bonferroni correction was applied to adjust for multiple comparisons. Bioinformatics analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment was also performed. We observed 20 Bonferroni significant (P-value < 9.4£ 10 ¡9 ) CpGs for Fe, 8 for Ni, and 1 for V. Particularly, methylation at Schlafen Family Member 11 (SLFN11) cg10911913 was positively associated with measured levels of all 3 species. The SLFN11 gene codes for an interferoninduced protein that inhibits retroviruses and sensitizes cancer cells to DNA-damaging agents. Bioinformatics analysis suggests that gene targets may be relevant to pathways including cancers, signal transduction, and cell growth and death. Ours is the first study to examine the epigenome-wide association between ambient particles species and DNA methylation. We found that long-term exposures to specific components of ambient particle pollution, especially particles emitted during oil combustion, were associated with methylation changes in genes relevant to immune responses. Our findings provide insight into potential biologic mechanisms on an epigenetic level.

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Cytohesin-1 regulates human blood neutrophil adhesion to endothelial cells through β2 integrin activation

Cited by 21 publications

References 42 publications

Supernatants and lipids from stored red blood cells activate pulmonary microvascular endothelium through the BLT2 receptor and protein kinase C activation

Supernatants and lipids from stored red blood cells activate pulmonary microvascular endothelium through the BLT2 receptor and protein kinase C activation

Cytohesin 1 regulates homing and engraftment of human hematopoietic stem and progenitor cells

Differential DNA methylation and PM_2.5 species in a 450K epigenome-wide association study

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Cytohesin-1 regulates human blood neutrophil adhesion to endothelial cells through β2 integrin activation

Cited by 21 publications

References 42 publications

Supernatants and lipids from stored red blood cells activate pulmonary microvascular endothelium through the BLT2 receptor and protein kinase C activation

Supernatants and lipids from stored red blood cells activate pulmonary microvascular endothelium through the BLT2 receptor and protein kinase C activation

Cytohesin 1 regulates homing and engraftment of human hematopoietic stem and progenitor cells

Differential DNA methylation and PM2.5 species in a 450K epigenome-wide association study

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Differential DNA methylation and PM_2.5 species in a 450K epigenome-wide association study