Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α

Jusman, Sri Widia; Iswanti, Febriana Catur; Suyatna, Franciscus D.; Ferdinal, Frans; Wanandi, Septelia Inawati; Sadikin, Mohamad

doi:10.13181/mji.v23i3.1025

Cited by 8 publications

(12 citation statements)

References 12 publications

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“…However, the free radicals were generated as the increased oxygen demand can stimulate the expression of HIF-1α. 18 This finding can explain the higher level of mRNA. However, in this period, the macrophages also actively synthesized and secreted several cytokine proteins.…”

Section: Discussionmentioning

confidence: 91%

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Efforts to overcome hypoxia condition in Balb/c mouse macrophages after intraperitoneal SRBC immunization

2019

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BACKGROUND Activated macrophages require increased oxygen to destroy foreign bodies, leading to an increase in the levels of reactive oxygen species (ROS). Therefore, macrophages would experience hypoxic and oxidative stress conditions at the same time. Thus, this study was aimed to evaluate the mechanism of the activated macrophages to overcoming this dual condition.METHODS The activated macrophages were harvested from the intraperitoneal cavities of 18 BALB/c mice immunized with 2% sheep red blood cells (SRBCs). The macrophage suspension was divided into four groups: control, 24, 48, and 72 hours after-immunization groups. The expressions of hypoxia-inducible factor (HIF)-1α, HIF-2α, and cytoglobin (Cygb), as markers for hypoxic condition, were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), whereas peroxisome proliferator-activated receptor gamma coactivator (PGC-1α) protein as a marker for mitochondrial biogenesis and aerobic metabolism was measured with ELISA. The analysis of oxidative stress was conducted with the water-soluble tetrazolium salt test.RESULTS The HIF-1α mRNA expression was the highest at 24 hours, whereas the HIF-2α mRNA showed no increased expression during the observation. The Cygb mRNA decreased after 24 hours. The highest expressions of HIF-1α and HIF-2α proteins were detected at 72 hours, whereas the Cygb protein expression increased since 24 hours. The PGC-1α protein expression increased at 72 hours. The WST test showed the highest ROS level at 24 hours.CONCLUSIONS The macrophages were activated by SRBCs underwent dual hypoxia and oxidative stress condition simultaneously to overcome the foreign bodies. The macrophages overcame these stress conditions by increasing their aerobic metabolism.

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Section: Discussionmentioning

confidence: 91%

“…This study has shown that Cygb is also under the control of HIF-1α. 18 PGC-1α is reported as a marker of biogenesis of mitochondria. 24 In our observation, the level of PGC-1 was lower than that of the control at 24 hours and was much higher in the later period.…”

Section: Discussionmentioning

confidence: 99%

Efforts to overcome hypoxia condition in Balb/c mouse macrophages after intraperitoneal SRBC immunization

2019

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“…According to Gauron, an imbalance in oxygen demand and oxygen supply causes tissues to become hypoxic [4]. Cygb can store oxygen and release it under hypoxic conditions, [9,12] so we suggest that Cygb is involved in the adaptive response to injury.…”

Section: I and 2s)mentioning

confidence: 82%

“…The ATPs fulfill their function in this process only when the O 2 supply is sufficient [5][6][7]. If the aerobic metabolism does not keep pace with the increase of oxygen demand, the tissue enters a relative hypoxia state [8,9]. In this state, the organisms strive to meet the oxygen demand in order to maintain the metabolism for cellular activities; hence, oxygen might be transferred to the mitochondria by the Cygb protein [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

High expressions of the cytoglobin and PGC-1α genes during the tissue regeneration of house gecko (Hemidactylus platyurus) tails

et al. 2020

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Background: The tissue regeneration process requires high oxygen and energy levels. Cytoglobin (Cygb) is a member of the globin family, which has the ability to bind oxygen, plays a role in dealing with oxidative stress, and carries oxygen into the mitochondria. Energy production for tissue regeneration is associated with mitochondriaespecially mitochondrial biogenesis. The peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha protein helps to regulate mitochondrial biogenesis. House geckos (Hemidactylus platyurus) are reptiles that have the ability to regenerate the tissue in their tails. House geckos were selected as the animal models for this study in order to analyze the association of Cygb with oxygen supply and the association of PGC-1α with energy production for tissue regeneration. Results: The growth of house gecko tails showed a slow growth at the wound healing phase, then followed by a fast growth after wound healing phase of the regeneration process. While Cygb mRNA expression reached its peak at the wound healing phase and slowly decreased until the end of the observation. PGC-1α mRNA was expressed and reached its peak earlier than Cygb. Conclusions: The expressions of both the Cygb and PGC-1α genes were relatively high compared to the control group. We therefore suggest that Cygb and PGC-1α play an important role during the tissue regeneration process.

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“…If the aerobic metabolism does not keep pace with the increase of oxygen demand, the tissue enters a relative hypoxia state. [(8), (9)] In this state, the organisms trive to meet the oxygen demand in order to maintain the metabolism for cellular activities; hence, oxygen might be transferred to the mitochondria by the Cygb protein. [10][11][12] 3…”

Section: Introductionmentioning

confidence: 99%

High expressions of the cytoglobin and PGC-1α genes during the tissue regeneration of house gecko (Hemidactylus platyurus) tails

Novianti

Juniantito²,

Jusuf

et al. 2020

Preprint

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Background The tissue regeneration process requires high oxygen and energy levels. Cytoglobin (Cygb) is a member of the globin family, which has the ability to bind oxygen, plays a role in dealing with oxidative stress, and carries oxygen into the mitochondria. Energy production for tissue regeneration is associated with mitochondria—especially mitochondrial biogenesis. The peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha protein helps to regulate mitochondrial biogenesis. House geckos ( Hemidactylus platyurus ) are reptiles that have the ability to regenerate the tissue in their tails. House geckos were selected as the animal models for this study in order to analyze the association of Cygb with oxygen supply and the association of PGC-1α with energy production for tissue regeneration. Results The growth of house gecko tails showed a slow growth at the wound healing phase, then followed by a fast growth after wound healing phase of the regeneration process. While Cygb mRNA expression reached its peak at the wound healing phase and slowly decreased until the end of the observation. PGC-1α mRNA was expressed and reached its peak earlier than Cygb. Conclusions The expressions of both the Cygb and PGC-1α genes were relatively high compared to the control group. We therefore suggest that Cygb and PGC-1α play an important role during the tissue regeneration process.

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Cytoglobin expression in oxidative stressed liver during systemic chronic normobaric hypoxia and relation with HIF-1α

Cited by 8 publications

References 12 publications

Efforts to overcome hypoxia condition in Balb/c mouse macrophages after intraperitoneal SRBC immunization

Efforts to overcome hypoxia condition in Balb/c mouse macrophages after intraperitoneal SRBC immunization

High expressions of the cytoglobin and PGC-1α genes during the tissue regeneration of house gecko (Hemidactylus platyurus) tails

High expressions of the cytoglobin and PGC-1α genes during the tissue regeneration of house gecko (Hemidactylus platyurus) tails

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