Cytogenotoxicity of Abattoir Effluent in<i> Clarias gariepinus</i> (Burchell, 1822) Using Micronucleus Test

Alimba, Chibuisi G.; Ajayi, E. O. B.; Hassan, Titilayo; Sowunmi, Akindayo A.; Bakare, Adekunle A.

doi:10.1155/2015/624524

Cited by 10 publications

(5 citation statements)

References 25 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 96 h LC50 value of 56% v/v found in the current investigation indicated that the vehicle wastewater is poisonous to the experimental fish. As in earlier research by Alimba et al (2015) in abattoir wastewater (96 h LC50 = 6.28%), mortality in the current finding was concentrationdependent. One of the enzyme preventing the body from the state of oxidative stress in living organisms is superoxide dismutase (SOD) (Mandeep and Rajinder, 2017).…”

Section: Discussionsupporting

confidence: 87%

Oxidative Stress Biomarkers Activities and Histological Changes in African Catfish (Clarias gariepinus) Exposed to Carwash Wastewater

Abdullahi¹,

Usman

Madaki³

et al. 2022

USci

View full text Add to dashboard Cite

The study evaluated the acute toxicity of vehicle wastewater on African catfish (Clarias gariepinus). With a weight range of 250–275.5g and a mean length of 23.5-28.5cm, 150 post-juvenile catfish were purchased from Dalar Kifi Fish Farm BUK New site road, Kano State, and acclimated in a tank for 14 days. Fish were exposed to different vehicle wastewater concentrations of 0.0, 20, 40, 60, and 80% v/v with 5-level exposure concentrations during the acute test (96 hours) in a Completely Randomized Design (CRD) employing renewal bioassay. The acute bioassay showed that the LC50 for the wastewater was 56 percent by volume at 96 hours. Compared to the control, the exposed fish exhibited abnormal swimming, erratic opercular movement, reflex loss, mucus secretion, increased air gulping with an increase in wastewater concentration. Except for water temperature, Biochemical Oxygen Demand (BOD) and pH, the remaining physicochemical parameters were not within the WHO/FAO recommended Standard The order of concentrations of the heavy metal in the wastewater was Cu > Pb > Cd > Cr > Ni. Antioxidant biomarker activities showed that Glutathione reductase (GSH) reduced in the same manner while Catalase (CAT) and Superoxide dismutase (SOD) activities rose considerably (P<0.05) in the liver and gill tissues. Histopathological changes observed in gill tissues were as follows: Hyperplasia, necrosis, primary and secondary lamellae distortion, and epithelial thickening distortion. Hepatic cell breakdown, fatty degeneration, cellular infiltration, localized fibrosis, and cellular necrosis were all visible in the liver tissues. It can be inferred that changes in the histology and activity of the oxidative stress enzymes in fish tissues after exposure to wastewater harmed the experimental fish. As a result of their effects on fish and other non-target aquatic biotas, it is advised that the competent authorities create initiatives to reduce the indiscriminate discharge of untreated vehicle wash wastewater.

show abstract

Section: Discussionsupporting

confidence: 87%

Oxidative Stress Biomarkers Activities and Histological Changes in African Catfish (Clarias gariepinus) Exposed to Carwash Wastewater

Abdullahi¹,

Usman

Madaki³

et al. 2022

USci

View full text Add to dashboard Cite

show abstract

“…The 96h LC50 value of the pharmaceutical effluents recorded in the present finding was 48.7% which depicted that the effluents are toxic to fish as reported by Agunbiace and Moodley (2016). Mortality recorded was dose and concentration-dependent as obtained in previous findings by Akan et al (2009) in tannery and textile effluents, Alimba et al (2015) in abattoir effluents (96 h LC50 = 6.28 %). Sublethal concentrations of the effluents may not cause immediate death of the aquatic biota, but the bioaccumulation of these pollutants for long period of time could have potential health impact not only to the aquatic biota such as fish but to organisms at higher trophic level including man as reported by Safahieh et al (2012).…”

Section: Discussionsupporting

confidence: 81%

In Vitro Effect of Pharmaceutical Effluent on Haematology and Biochemical Responses in African Catfish (Clarias Gariepinus)

Abdullahi

Zakariyya²,

Hassan³

et al. 2021

FJS

View full text Add to dashboard Cite

Pharmaceutical effluents collected from Bompai industrial area, Kano State were assessed for their physiochemical parameters, heavy metals and effect on haematology and biochemical changes in Clarias gariepinus. Laboratory analyses were performed using standard methods in a Completely Randomized Design (CRD). Five test solutions of the effluents (0, 25, 50, 75, and 100% v/v) were prepared for LC50 96hr acute toxicity test. The LC50 for 96hr was 48.7% concentration of effluents by volume. Experimental fish were exposed to sublethal concentrations of 0.00% 2.43% (5/100 LC50), 12.17% (25/100 LC50), 24.35% (50/100 LC50) and 36.52% (75/100 LC50) for 28days. Physicochemical parameters recorded were higher than WHO recommended Standard with the exception of water temperature. Heavy metals concentrations decreased in the order of Cr > Cu > Pd > Cd. Red blood Cells count, haemoglobin concentrations, packed cell volume, lymphocytes and monocytes of the experimental fish decline significantly (p<0.05) when exposed with 2.43, 12.17, 24.35, 36.52% effluents compared to the control. White blood cell count, mean corpuscular haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin concentrations, neutrophils and eosinophils were significantly higher (P<0.05) than the control. However, a significant increase (P<0.05) in the activities of serum aspartate aminotransferase (AST), Alkaline Phosphatase (ALP) and alanine aminotransferase (ALT) were recorded as the exposure period continued when compared with control. It is concluded that the effluents induces haematological and biochemical alterations. It is therefore recommended that regulatory bodies should adopt holistic approach on the aquatic pollution abatement, bearing in mind the negative impact to non-target organisms.

show abstract

“…also documented the dose specific induction of MN and NAs in four species of fish exposed to arsenic for a period of 30 days Al-Tamimi et al (2015). noted the progressive increase in MN formation in erythrocytes of Cyprinus carpio as the concentration of copper exposure increased Alimba et al (2015). also noted the time dependent formation of MN and NAs frequency in blood of Clarias gariepinus exposed to effluent from Bodija, Nigeria for 7-, 14-and 28-day Ashmawy et al (2015).…”

mentioning

confidence: 94%

Micronucleus Assay as a Biomarker to Diagnose Lead, Chromium and Cadmium Induced Genotoxicity in Erythrocytes of Carnivorous Fish, Wallago attu

Abdullah¹,

Naz²,

Abbas³

et al. 2021

PUJZ

View full text Add to dashboard Cite

Heavy metals discharged from agriculture and industries into water bodies are a significant cause of water contamination. This increase in heavy metals cause genetic damage in exposed species like fish. Therefore, this study focused on the genotoxic effects of lead (Pb), chromium (Cr) and cadmium (Cd) on nuclear abnormalities in peripheral erythrocytes of Wallago attu were assessed by micronucleus assay. Fish were exposed to different concentrations (1/3 rd , 1/4 th , 1/5 th and 1/7 th of LC 50 ) of Pb, Cr and Cd, separately, for three weeks. Blood sample of fish was collected to see the micronuclei (MN) and deshape nuclei (DN) in peripheral erythrocytes of fish after one-week interval. Fish W. attu showed significant induction of MN and DN in blood due to metals (Pb, Cr and Cd) exposure. It was noted that Pb and Cd exposure caused significant formation of MN and DN in RBCs of fish throughout the experimental period. However, Cr treated fish showed higher formation of MN and DN during initial two weeks of exposure after that both were decreased. The toxic potential of different concentration of Pb, Cr and Cd induced genotoxicity followed order: 1/3 rd >1/4 th >1/5 th >1/7 th of LC 50 . The metal specific response of fish followed the trend: Pb>Cr>Cd. Statistical analysis revealed that different concentrations and duration of Pb, Cr and Cd have significant effect on the frequency of micro-nucleated erythrocytes. The inferences of this study will be helpful in monitoring aquatic ecosystems using fish biomarkers.Novelty Statement | The study is novel for detecting the genetic damage at cellular level of fish (Wallago attu) caused by heavy metals and industrial toxicants in water.

show abstract

Cytogenotoxicity of Abattoir Effluent in Clarias gariepinus (Burchell, 1822) Using Micronucleus Test

Cited by 10 publications

References 25 publications

Oxidative Stress Biomarkers Activities and Histological Changes in African Catfish (Clarias gariepinus) Exposed to Carwash Wastewater

Oxidative Stress Biomarkers Activities and Histological Changes in African Catfish (Clarias gariepinus) Exposed to Carwash Wastewater

In Vitro Effect of Pharmaceutical Effluent on Haematology and Biochemical Responses in African Catfish (Clarias Gariepinus)

Micronucleus Assay as a Biomarker to Diagnose Lead, Chromium and Cadmium Induced Genotoxicity in Erythrocytes of Carnivorous Fish, Wallago attu

Contact Info

Product

Resources

About