Cytogenetics of Tephrosia I. On the differential chromosome pairing in colchiploid stock of Tephrosia wallichii.

Srivastav, P. K.; Raina, S. N.

doi:10.1508/cytologia.46.89

Cited by 3 publications

(10 citation statements)

References 18 publications

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“…It has been observed, as reported eralier by Raghuvanshi and Joshi (1965), that the seed treatment was always met with complete failure. Similar observations were made in a number of legumes by different authors (Sen and Vidyabushan 1960, Gupta and Gupta 1975, Srivastav 1983. In this case the present authors are in complete agreement with Bates (1939) and Srivastav (1983) that a check on root development and/or germination results in the failure of seed-treatment method to induce autotetraploidy.…”

Section: Discussionsupporting

confidence: 92%

“…Similar observations were made in a number of legumes by different authors (Sen and Vidyabushan 1960, Gupta and Gupta 1975, Srivastav 1983. In this case the present authors are in complete agreement with Bates (1939) and Srivastav (1983) that a check on root development and/or germination results in the failure of seed-treatment method to induce autotetraploidy. Though partial success was achieved by cotyledonary leaves immersion method, it is not much useful because percentage of success is as low as 6.25.…”

Section: Discussionsupporting

confidence: 92%

“…The reduced growth rate and over-all development of the plants in Co generation which appeared to gain normalcy through C1, C2 and C3 generations, is a common feature associated with autopolyploids and is reported in a number of plants (Chandler and Barton 1955, Zadoo et al 1975, Raghuvanshi and Singh 1977, Srivastav 1983. The causative factors are presumed to be the reduced rate of cell division (Wettstein 1924, Eigsti 1947 and reduction in the rate of metabolic activities (Larsen 1943, Schwanitz 1951.…”

Section: Discussionmentioning

confidence: 99%

“…But, for some unknown reasons, induction of polyploidy in legumes was found rather difficult. A review of literature reveals that very few reports on suc cessful induction of polyploidy in legumes (Sen and Chedda 1958, Biswas and Bhattacharya 1971, Singh and Roy 1971, Gupta and Gupta 1975, 1976, Raghuvanshi and Singh 1977, Singh and Gopal 1979 are available in comparison to large number of reports on popular ornamentals Jalil et al 1974, Arora 1975, Zadoo et al 1975, Srivastav and Raina 1981, Rao et al 1982.Trigonella foenum-graecum (commonly known as "Methi" or "Fenugreek") of family Leguminosae and subfamily Papilionaceae, is extensively grown in tropical and subtropical regions of India for its vegetable value and seeds which are medicinally important. The present study was undertaken in a view to study the cytomorphological features of T. foenum -graecum polyploids in successive generations and to explore the possibility of evolving a high yielding variety in terms of vegetable value and improved seed-set.…”

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Cytomorphological studies of Trigonella foenum-graecum autotetraploids in three (C1,C2,C3) generation.

1988

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Successful induction of polyploidy using colchicine is a well established phenomenon and still remains a potential cytological technique to induce numerical variations in plants. Since the discovery of Blakslee and Avery in 1937 about the chromosome doubling property of colchicine, enormous number of papers have been published on colchicine induced polyploidy. However, this technique was found to be more promising in popular ornamentals and veg etatively propagated plants. But, for some unknown reasons, induction of polyploidy in legumes was found rather difficult. A review of literature reveals that very few reports on suc cessful induction of polyploidy in legumes (Sen and Chedda 1958, Biswas and Bhattacharya 1971, Singh and Roy 1971, Gupta and Gupta 1975, 1976, Raghuvanshi and Singh 1977, Singh and Gopal 1979 are available in comparison to large number of reports on popular ornamentals Jalil et al. 1974, Arora 1975, Zadoo et al. 1975, Srivastav and Raina 1981, Rao et al. 1982.Trigonella foenum-graecum (commonly known as "Methi" or "Fenugreek") of family Leguminosae and subfamily Papilionaceae, is extensively grown in tropical and subtropical regions of India for its vegetable value and seeds which are medicinally important. The present study was undertaken in a view to study the cytomorphological features of T. foenum -graecum polyploids in successive generations and to explore the possibility of evolving a high yielding variety in terms of vegetable value and improved seed-set. Material and methodsSeeds of T. foenum-graecum used for colchicinization were obtained from Central Arid Zone Research Institute, Jodhpur.For colchicinization three methods viz. seed treatment, cotton-swab and cotyledonary leaves immersion methods were tried. The treatment was carried out for 12 or 18 hrs spread over 2 or 3 days with 0.15% colchicine (Sigma). For meiotic analysis buds were collected and fixed in iron acetic-alcohol overnight. Anthers stained in leucobasic-fuchsin were squashed in 1% aceto-carmine. Microphotographs were taken from temporary preparations. ObservationsOf the all the three methods employed, successful induction of polyploidy was achieved mainly by cotton swab method and partially by cotyledonary leaves immersion method (Table 1). Seed germinated after the treatment. Out of seventy seedlings treated by cotton-swab method with 0.15% colchicine for 12 or 18 hrs equally spread over three days, six were cyto logically confirmed as autotetraploids. Only one out of 22 seedlings treated for 10 hrs by cotyledonary leaves immersion method was found to be autotetraploid on cytological analysis.Swelling of the apical region of the seedlings was the immediate effect of coichinization,

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Cytomorphological studies of Trigonella foenum-graecum autotetraploids in three (C1,C2,C3) generation.

1988

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Cytogenetics of Clitoria. I. Induced autotetraploidy in C. ternatea.

Srivastav

Raina

1982

CYTOLOGIA

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Induction and evaluation of colchitetraploids of two species of Tinospora Miers, 1851

Thakur¹,

Rajpal

Rao³

et al. 2020

CCG

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Autotetraploidy, both natural and/or induced, has potential for genetic improvement of various crop species including that of medicinal importance. Tinospora cordifolia (Willdenow, 1806) Miers, 1851 ex Hooker et Thomson, 1855 and T. sinensis (Loureiro, 1790) Merrill, 1934 are two diploid species, which are dioecious, deciduous and climbing shrubs with high medicinal importance. Among the three methods used for induction of polyploidy by colchicine treatment, it was cotton swab method which successfully induced the polyploidy in both species. The morphological and cytogenetical features of the synthetic tetraploids were compared with their diploid counterparts. The tetraploids were morphologically distinct from diploid plants. They exhibited larger organs, such as stem, leaves, inflorescence, fruits, flowers and seeds. The tetraploids were characterized by the presence of low quadrivalent frequency and high bivalent average. Unequal distribution of chromosomes at anaphase I was found in 60% cells. The present study provides important information on the superiority of autotetraploids as compared to diploid counterparts in both species.

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Cytogenetics of Tephrosia I. On the differential chromosome pairing in colchiploid stock of Tephrosia wallichii.

Cited by 3 publications

References 18 publications

Cytomorphological studies of Trigonella foenum-graecum autotetraploids in three (C1,C2,C3) generation.

Cytomorphological studies of Trigonella foenum-graecum autotetraploids in three (C1,C2,C3) generation.

Cytogenetics of Clitoria. I. Induced autotetraploidy in C. ternatea.

Induction and evaluation of colchitetraploids of two species of Tinospora Miers, 1851

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