Cytogenetics of Porcine Sperm Chromosomes Following IVF of Zona-Free Hamster Oocytes

Bird, Jeremy; Houghton, J. A.

doi:10.3109/01485019008987593

Cited by 3 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only scarce data on the frequency of aneuploidy in boar sperm obtained by the zona-free hamster oocyte assay are available. Bird and Houghton (1990), who tested 155 sperm collected from two boars, reported a 2.5 % frequency of disomy. Our data, derived from investigations by FISH of three porcine sperm chromosomes, indicate a 0.078 % frequency of disomy per chromosome.…”

Section: Discussionmentioning

confidence: 99%

“…A similar hamster-egg fusion assay has been developed for investigating sperm obtained from farm animals. However, most of the papers on this assay deal with methodology, and the total number of tested spermatozoa is only about several hundred (Creighton and Houghton, 1987;Kovacs and Foote, 1989;Bird and Houghton, 1990;Benkhalifa et al, 1992). Nevertheless, the limited data available from this method suggest that the percentage of aneuploid sperm is similar in boars and man (Bird and Houghton, 1990).…”

mentioning

confidence: 99%

“…However, most of the papers on this assay deal with methodology, and the total number of tested spermatozoa is only about several hundred (Creighton and Houghton, 1987;Kovacs and Foote, 1989;Bird and Houghton, 1990;Benkhalifa et al, 1992). Nevertheless, the limited data available from this method suggest that the percentage of aneuploid sperm is similar in boars and man (Bird and Houghton, 1990). Apart from the methodological papers published by Kawarasaki et al (1995Kawarasaki et al ( , 1996, the potential of fluorescence in situ hybridization (FISH) as a method of investigating aneuploidy in farm animal sperm has yet to be exploited.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Aneuploidy in pig sperm: multicolor fluorescence in situ hybridization using probes for chromosomes 1, 10, and Y

Rubeš

Vozdová

Kubı́čková

1999

Cytogenet Genome Res

View full text Add to dashboard Cite

The objective of this research was to develop chromosome-specific probes for use in evaluating aneuploidy in boar spermatozoa through the application of fluorescence in situ hybridization (FISH) technology. A multicolor FISH method was developed to detect aneuploidy in the sperm of boars using DNA probes specific for small regions of chromosomes 1, 10, and Y. The average frequencies of sperm with disomy for chromosomes 1, 10, and Y were 0.075%, 0.067%, and 0.094%, respectively. The incidence of disomy did not differ significantly by chromosome. The average frequencies of diploidy were 0.177% for 1-1-10-10 and 0.022% for Y-Y-10-10. Thus, the incidence of overall diploidy (1-1-10-10) was significantly higher than that of disomy for the chromosomes examined (P < 0.01 for disomy of the autosomes and P < 0.05 for disomy of the Y chromosome). No significant age or breed effects on disomy and diploidy rates and no significant interindividual variations in disomy or diploidy were found. The observed level of numerical chromosome aberrations in pig sperm appear to be within the range of the baseline frequencies reported so far in men.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Aneuploidy in pig sperm: multicolor fluorescence in situ hybridization using probes for chromosomes 1, 10, and Y

Rubeš

Vozdová

Kubı́čková

1999

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

A freezing and thawing method of hamster oocytes designed for both the penetration test and chromosome assay of human spermatozoa

Tateno

Kamiguchi

Mikamo

1992

Molecular Reproduction Devel

View full text Add to dashboard Cite

Superovulated hamster oocytes were cryopreserved and thawed according to our carefully designed procedures. More than 90% (92 +/- 4%) of oocytes survived freezing and thawing. They were proven to be well conserved, showing excellent performance comparable to freshly ovulated oocytes in the human sperm penetration test (proportion of penetrated ova: 94.7% vs. 93.6%) and human sperm chromosome analysis (proportion of metaphasic ova: 81.8% vs. 83.6%). There was no statistically significant difference in the incidences of sperm chromosome aberrations between assays using fresh and frozen-thawed oocytes. In addition, there was no statistically significant increase of aberrations in female pronuclear (hamster) chromosomes. This freezing-thawing method was found to be reliable, yielding viable hamster oocytes of high quality.

show abstract

Chromosome Aberrations and Fertility Disorders in Domestic Animals

Raudsepp

Chowdhary

2016

Annu. Rev. Anim. Biosci.

View full text Add to dashboard Cite

The association between chromosomal abnormalities and reduced fertility in domestic animals is well recorded and has been studied for decades. Chromosome aberrations directly affect meiosis, gametogenesis, and the viability of zygotes and embryos. In some instances, balanced structural rearrangements can be transmitted, causing fertility problems in subsequent generations. Here, we aim to give a comprehensive overview of the current status and future prospects of clinical cytogenetics of animal reproduction by focusing on the advances in molecular cytogenetics during the genomics era. We describe how advancing knowledge about animal genomes has improved our understanding of connections between gross structural or molecular chromosome variations and reproductive disorders. Further, we expand on a key area of reproduction genetics: cytogenetics of animal gametes and embryos. Finally, we describe how traditional cytogenetics is interfacing with advanced genomics approaches, such as array technologies and next-generation sequencing, and speculate about the future prospects.

show abstract

Cytogenetics of Porcine Sperm Chromosomes Following IVF of Zona-Free Hamster Oocytes

Cited by 3 publications

References 18 publications

Aneuploidy in pig sperm: multicolor fluorescence in situ hybridization using probes for chromosomes 1, 10, and Y

Aneuploidy in pig sperm: multicolor fluorescence in situ hybridization using probes for chromosomes 1, 10, and Y

A freezing and thawing method of hamster oocytes designed for both the penetration test and chromosome assay of human spermatozoa

Chromosome Aberrations and Fertility Disorders in Domestic Animals

Contact Info

Product

Resources

About