Cytogenetics of bladder cancer

Gibas, Zenon; Gibas, Longina

doi:10.1016/s0165-4608(96)00295-6

Cited by 29 publications

(18 citation statements)

References 52 publications

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“…All examined tumours showed cytogenetic aberrations involving the short and/or long arm of chromosome 9, and indeed the importance of chromosome 9 rearrangement as an early, presumably primary, event in BC tumorigenesis is widely accepted (Miyao et al, 1993;Gibas et al, 1997). The ubiquity of chromosome 9 changes in our series may be contrasted with the absence Ta/G1-2 46,X,t(X;9)(p11;p11),del(1)(p11),+del(1)(p11), del(7)(q11),i(8)(q10),add(9)(q12),der(11)t(7;11) (q11;p15)del(11)(q12),der(13)t(1;13)(p11;p11), del(13)(q13q22),-14[100] BT6/Bladder Ta/G1-2 46,X,t(X;9)(p11;p11),del(1)(p11),+del(1)(p11), del(7)(q11),i(8)(q10),add(9)(q12),der(11)t(7;11) (q11;p15)del(11)(q12),der(13)t(1;13)(p11;p11), del(13)(q13q22),-14[100] BT7/Bladder T1/G3 46,X,t(X;9)(p11;p11),del(1)(p11),+del(1)(p11), del(7)(q11),add(9)(q12),der(11)t(7;11)(q11;p15) del(11)(q12),der(13)t(1;13)(p11;p11),-14[30]/ 46,X,t(X;9)(p11;p11),del(1)(p11),del(1)(p11), del(7)(q11),i(8)(q10),add(9)(q12),der(11)t(7;11) (q11;p15)del(11)(q12),der(13)t(1;13)(p11;p11), del(13)(q13q22),-14[40]/ 46,X,t(X;9)(p11;p11),del(1)(p11),+del(1)(p11), del(7)(q11),i(8)(q10),add(9)(q12),der(11)t(7;11) (q11;p15)del(11)(q12),der (13) Ta/G2 89-94,XXYY,add(6)(p21),add(8)(p11), del(8)(p22),-9,add(16)(p11),der(17)t(1;17) (q12;p11),-1+1-2mar[cp25] RT10/Bladder Ta/G2 89-93,XXYY,add(6)(p21),add(8)(p11), del(8)(p22),-9,add(16)(p11),der(17)t(1;17) (q12;p11),+mar [cp30] of abnormalities affecting chromosome 17 in most of the tumours, in spite of the fact that such changes, too, are common in bladder carcinomas, especially in invasive poorly differentiated TCCs (Fujimoto et al, 1992;Grossfeld et al, 1997).…”

Section: Discussionmentioning

confidence: 96%

Cytogenetic monoclonality in multifocal uroepithelial carcinomas: evidence of intraluminal tumour seeding

et al. 1999

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Summary Twenty-one multifocal urinary tract transitional cell carcinomas, mostly bladder tumours, from a total of six patients were processed for cytogenetic analysis after short-term culturing of the tumour cells. Karyotypically related, often identical, cytogenetically complex clones were found in all informative tumours from each case, including the recurrent tumours. Rearrangement of chromosome 9, leading to loss of material from the short and/or the long arm, was seen in all cases, indicating that this is an early, pathogenetically important event in transitional cell carcinogenesis. The presence of related clones with great karyotypic similarity in anatomically distinct tumours from the same bladder indicates that multifocal uroepithelial tumours have a monoclonal origin and arise via intraluminal seeding of viable cancer cells shed from the original tumour. Later lesions may develop also from cells shed from the so called second primary tumours. The relatively complex karyotypes seen in all lesions from most cases argue that the seeding of tumour cells is a late event that succeeds the acquisition by them of multiple secondary genetic abnormalities.

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Section: Discussionmentioning

confidence: 96%

Cytogenetic monoclonality in multifocal uroepithelial carcinomas: evidence of intraluminal tumour seeding

et al. 1999

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“…The subsequent SKY analysis also found only a normal male karyotypic complement in Case 6, but a small clone (3 cells) with monosomy for chromosome 9 in Case 4. The biological significance of this finding must be viewed against the background that monosomy 9 has been repeatedly described as the sole chromosomal change in uroepithelial carcinomas, 14,18 which is strong, albeit indirect, evidence of the malignant nature of the analyzed cells. Monosomy 9 is equally well detectable in G-banded and SKY stained preparations, and the case thus illustrates that some of the discrepant results obtained by G-banding and SKY may be attributed to the fact that different populations of dividing cells were analyzed in the 2 experiments.…”

Section: Maps Of Breakpoints and Imbalancesmentioning

confidence: 96%

“…15,18 TCCs may have simple chromosomal changes only or highly complex karyotypes. Balanced translocations are rarely seen in these karyotypes that are dominated by nonrandom chromosome deletions and losses of entire chromosome copies indicating that losses of tumor suppressor genes are the most important pathogenetic events.…”

Section: Maps Of Breakpoints and Imbalancesmentioning

confidence: 99%

Characterization of chromosomal abnormalities in uroepithelial carcinomas by G-banding, spectral karyotyping and FISH analysis

et al. 2001

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Chromosome analysis by G-banding, spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) was performed on 24 short-term cultured transitional cell bladder carcinomas and 5 cell lines established from bladder carcinomas. Except for one tumor with an apparently normal chromosomal constitution, clonal chromosome abnormalities were detected in all examined cases by the combined approach. The application of SKY and FISH techniques improved the karyotypic descriptions, originally based on G-banding only, by identifying 32 additional numerical changes, by establishing the chromosomal origin of 27 markers and 2 ring chromosomes, by redefining 53 aberrations and by detecting 15 hidden chromosomal rearrangements. No recurrent translocation, however, was detected. The most prominent karyotypic feature was thus the occurrence of deletions and losses of whole chromosome copies indicating the importance of tumor suppressor genes in transitional cell carcinoma pathogenesis. Invasive carcinomas were karyotypically more complex than were low grade superficial tumors. Specific losses of material from chromosome 9 and from chromosome arms 11p and 8p, and gains of 8q and 1q seem to be early changes appearing in superficial tumors, whereas losses from 4p and 17p and the formation of an isochromosome for 5p were associated with more aggressive tumor phenotypes.

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“…Cytogenetic and molecular studies have identified genetic alterations on chromosome 11p as one of the most frequent events during the progression of bladder cancer (Habuchi et al, 1993;Shaw and Knowles, 1995;Voorter et al, 1996;Gibas and Gibas, 1997). Deletion mapping has revealed a common region of deletion between D11S922 (11p15.5) and D11S569 (11p15.1-11p15.2), but it is not yet known which particular gene constitutes the relevant target.…”

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confidence: 99%

Decreased expression of p57KIP2 mRNA in human bladder cancer

Oya

Schulz

2000

Br J Cancer

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Summary To identify targets of genetic and epigenetic alterations on chromosome 11p15.5 in human bladder cancer, expression of the imprinted KIP2, IGF2 and H19 genes was studied by quantitative RT-PCR in 24 paired samples of urothelial carcinomas and morphologically normal mucosa obtained by cystectomy, and in bladder carcinoma cell lines. The most frequent alteration in tumour tissue was decreased expression of KIP2 identified in 9/24 (37%) specimens. Decreased IGF2 and H19 mRNA levels were found in five (21%) and three (13%) tumours, respectively. One tumour each overexpressed IGF2 and H19. Loss of H19 expression was only found associated with loss of KIP2 expression, whereas decreased expression of IGF2 mRNA occurred independently. Almost all bladder carcinoma cell lines showed significant changes in the expression of at least one gene with diminished expression of KIP2 mRNA as the most frequent alteration. IGF2 mRNA levels were diminished in several lines, but increased in others. The KIP2 gene could be an important target of genetic and epigenetic alterations in bladder cancer affecting the maternal chromosome 11p15.5. However, reminiscent of the situation in Wilms' tumours, expression of the IGF2 gene on the paternal chromosome can also be disturbed in bladder cancers.

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Cytogenetics of bladder cancer

Cited by 29 publications

References 52 publications

Cytogenetic monoclonality in multifocal uroepithelial carcinomas: evidence of intraluminal tumour seeding

Cytogenetic monoclonality in multifocal uroepithelial carcinomas: evidence of intraluminal tumour seeding

Characterization of chromosomal abnormalities in uroepithelial carcinomas by G-banding, spectral karyotyping and FISH analysis

Decreased expression of p57KIP2 mRNA in human bladder cancer

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