Cytogenetic toxicity of methotrexate in mouse bone marrow

Choudhury, Ramesh C.; Ghosh, Subir; Palo, Anil K.

doi:10.1016/s1382-6689(00)00041-7

Cited by 41 publications

(20 citation statements)

References 18 publications

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“…Methotrexate (Mtx), an antifolate, is used as an anti-cancer drug in higher doses such as lymphoma, breast carcinoma, sarcoma, breast cancer (Choudhury et al, 2000;Miyazaki et al, 2003;Seigers et al, 2008;Findlay et al, 2008;D'Adamo, 2011) and also in lower doses in the treatment of rheumatic diseases, psoriasis, refractory inflammatory bowel disease since it has immunosuppressant effect (Peters et al, 2000;Gisondi and Girolomoni, 2007;Renna, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effects of Lycopene Alone or Combined with Melatonin on Methotrexate-Induced Nephrotoxicity in Rats

Oğuz

Koçarslan²,

Tabur³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Methotrexate (Mtx), used for its anticancer and immunsuppresive properties, is known to be a nephrotoxic agent. We aimed to investigate the effects of lycopene (Lyc) alone or combined with melatonin (Mel) on Mtxinduced nephrotoxicity since both of these agents have antioxidant and anti-inflammatory effects. Nephrotoxicity was induced by intraperitoneal administration of methotrexate at a dose of 20 mg/kg. Treatment both with Lyc alone and Lyc combined with Mel provided significant reduction in tumor necrosis factor-alpha, interleukin 1-beta and ceruloplasmin levels in Mtx administered rats. Hovewer, Lyc combined with Mel provided a significant reduction also in NO levels. Hstopathological examination showed that there was an obvious improvement in the degenerative changes compared to Mtx administrated group with the Lyc combined Mel group giving best protection. In conclusion Lyc alone and combined with Mel provided significant improvement against renal damage caused by Mtx, preseumably via antioxidant and anti-inflammatory activities.

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Section: Introductionmentioning

confidence: 99%

Effects of Lycopene Alone or Combined with Melatonin on Methotrexate-Induced Nephrotoxicity in Rats

Oğuz

Koçarslan²,

Tabur³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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“…Morphological mutations observed were: degradation of cell nucleus, cytoplasm destruction and cell lysis. Among available reports on the genotoxicity assessments of methotrexate in literature, that seemed to be rather scarce, there is a limited information on the potential cytotoxic and genotoxic effects of methotrexate in eukaryotic systems (Finnete, et al 2000, Choudhury, et al 2000Rice et al 2004). Based on results from this study, it can be said that methotrexate has potential genotoxic and cytotoxic effects on genetic material.…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity analysis of Fromilid and Methotrexate using Allium test

Cenanovic

2014

scjournal

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Ground Moving Target Indicator (GMTI) and High Resolution Radar (HRR) can track position and velocity of ground moving target. Pose, angle between position and velocity, can be derived from kinematics estimates of position and velocity and it is often used to reduce the search space of a target identification (ID) and Automatic Target Recognition (ATR) algorithms. Due to low resolution in some radar systems, the GMTI estimated pose may exhibit large errors contributing to a faulty identification of potential targets. Our goal is to define new methodology to improve pose estimate. Besides applications in target tracking, there are numerous commercial applications in machine learning, augmented reality and body tracking.

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“…It has been prescribed extensively as a cytotoxic agent in the treatment of leukemia, breast cancer, testicular tumors and other malignancies. It is used in psoriasis and rheumatoid arthritis that are inflammatory related diseases [2][3][4]. MTX causes some toxic effects including nausea, vomiting, diarrhea, alopecia, stomatitis, bone marrow depression and hepatotoxicity in patients [5].…”

Section: Introductionmentioning

confidence: 99%

The use of 99mTc-phytate for assessment the protective effect of vitamin E against hepatotoxicity induced by methotrexat in rat

et al. 2018

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Hepatotoxicity is one of the most common side effects of methotrexate (MTX) therapy in patients. The aim of this study was to evaluate the protective effect of vitamin E against MTX-induced hepatotoxicity using 99m Tc-phytate as a radiopharmaceutical agent in animals. Rats were divided into five groups as follows: control, solvent, Vit E (100 mg/kg), MTX (20 mg/kg) and Vit E + MTX. Animals were intraperitoneally injected with Vit E for 17 days before MTX injection and continued for 4 days. Tc-phytate was injected into the tail of rats after the 21 days of Vit E administration. Percentage of the injected dose per gram of liver and spleen tissues (%ID/g) was calculated in treated rats. Liver imaging was obtained with gamma camera. In other experiment, liver of treated rats was assessed for histopathology. Tc-phytate uptake (%ID/g) of livers in control, solvent, Vit E, MTX and Vit E + MTX groups were 8.99% ± 1.37, 8.53% ± 2.91, 8.65% ± 3.84, 3.22% ± 1.09 and 8.38% ± 2.68. Vit E administration resulted in a significant increase of the level of %ID/g in MTX-injected animal. Vit E pre-treatment improved the shape of liver in MTX-treated rat which was seen abnormal view in planar imaging. Histophatological examinations approved the protective effect of Vit E against MTX-induced hepatotoxicity in rats. The results of this study show that Vit E significantly attenuates the MTX-induced hepatotoxicity in rats, and 99m Tc-phytate is an acceptable radiopharmaceutical agent for assessment of liver and spleen damages in the animal model.

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Cytogenetic toxicity of methotrexate in mouse bone marrow

Cited by 41 publications

References 18 publications

Effects of Lycopene Alone or Combined with Melatonin on Methotrexate-Induced Nephrotoxicity in Rats

Effects of Lycopene Alone or Combined with Melatonin on Methotrexate-Induced Nephrotoxicity in Rats

Genotoxicity analysis of Fromilid and Methotrexate using Allium test

The use of 99mTc-phytate for assessment the protective effect of vitamin E against hepatotoxicity induced by methotrexat in rat

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