Cytogenetic Studies on the Effect of Co&lt;sup&gt;60&lt;/sup&gt; γ-Irradiation on &lt;i&gt;Vicica faba&lt;/i&gt;

Amer, Soheir M.; Mikhael, Evon

doi:10.1508/cytologia.37.169

Cited by 5 publications

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“…AIthough the production of micronuclei in root meristemcells is well established (Soran et al 1981;Degrassi and Rizzoni, 1982;Fayed andHesemann, 1983 andTang et al (1984), similar studies in meiotic divisions are relatively few. Das et al 1968;Reddy, 1971 andMikhael, 1972 observed micronuclei in PMC's of Vicia faba originated from seeds treatment with mutagenic agent. These authors considered micronuclei as a feature of meiotic abnormality.…”

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confidence: 99%

Micronuclei, as Indicator of Mutagenecity In Cultivated Allium

Soliman¹

1999

Pakistan J. of Biological Sciences

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This study was performed to determine the relationship between chromosome aberrations in PMCs and micronuclei formation as well as chiasma frequency in cultivated Allium cepa collected from two sites. One site adjacent to the high way which is characterize by heavy traffic and the plants grown exposed to the different components of ignition exhaust in air as well as in soil, The other site away from the traffic pollution as control samples. Different types of chromosomal aberrations observed in first and second meiotic cells were: bridge, stickiness, chromosome aggregates in the form of a ring, vaculated nucleus, misorientation and multinucleated cells. Micronuclei formation represented the most dominant type of mieiotic abnormalities, The results indicated that aberration percentages of meiosis I were more frequent than those of meiosis II. Also a decrease in chiasma frequency in site I over site II was observed.

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Section: Introductionmentioning

confidence: 99%

Micronuclei, as Indicator of Mutagenecity In Cultivated Allium

Soliman¹

1999

Pakistan J. of Biological Sciences

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Irradiation Studies on the Karyology of Charophyta

Sarma

Singh

1977

CYTOLOGIA

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Effect of electric field on mitosis in root tips of Allium cepa L.

Saxena

Gupta

1987

CYTOLOGIA

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The process of mitosis and the mitotic chromosomes are known to be affected by ionising and non-ionising radiations (Sax 1941, Evans 1962, Amer and Mikhael 1972 , Sharma and Singh 1974, a wide range of chemicals including growth substances, insectisides , herbicides and fungicides (Doxey 1949, Prasad andDas 1977) and magnetic field (Goswami 1977) . The present paper deals with the effect of electric field on the process of somatic cell division. Materials and methodsSeeds of Allium cepa L. were grown in petridishes on moist filter papers and those with approximately 5mm long and straight radicles were selected. A pair of equal sized plastic petriplates was taken. The lower half was lined with moist cotton, sufficient to keep the chamber humid. It was covered with a cellophane sheet which had several small perforations at regular intervals. The germinating Allium seeds were arranged on this sheet such that their radicles penetrated vertically downwards through the perforations. This was covered by the other petriplate. Two thin metallic sheets were tightly fixed by cellophane tape on the top and bottom of this arrangement (Fig. 1).A uniform electric field was generated by joining these two metallic sheets with the two terminals of D.C. power supply. The intensity of this electric field was regulated by regulating the potential difference between the two points. The experiment was set at 8a.m. in the morn ing and continued for 2 hours. After the treatment the root tips were collected and fixed in 1:3 acetoalcohol with traces of ferric chloride for 24 hours and preserved in 70% ethanol. Observations were made from acetocarmine squash preparations. Photomicrographs were taken from the temporary slides which were later made permanent. ResultsThe mitotic indices, the frequencies of cells at different karyokinetic stages as well as the frequencies of cells showing different types of chromosomal aberrations at each of these stages are presented in the Table. The mitotic index was 10.8 in control, 10.6 in the first treatment and 11.1 in the second treatment. Cells under the process of division during the treatments showed various abnor malities. The spindle organisation was conspicuously disturbed at metaphase. Almost 88 of the metaphases studied showed that the chromosomes failed to arrange their kinetochores on the metaphase plate resulting in unoriented fibrillar structures of the spindles (Fig. 2).Although, chromosomal fragmentation occurred at metaphase also, this effect was more pronounce at anaphase (Figs. 3, 4). Fragments were often accompanied by one or more bridges (Fig. 5). A high frequency of laggards was also found at this stage (Figs. 6, 7, 8). Besides, certain other abnormalities, viz, loops (Figs. 9, 10), dicentric rings (Fig. 11) and eroded chromosomes (Fig. 12) were also observed in few cells.

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Cytogenetic Studies on the Effect of Co<sup>60</sup> γ-Irradiation on <i>Vicica faba</i>

Cited by 5 publications

References 5 publications

Micronuclei, as Indicator of Mutagenecity In Cultivated Allium

Micronuclei, as Indicator of Mutagenecity In Cultivated Allium

Irradiation Studies on the Karyology of Charophyta

Effect of electric field on mitosis in root tips of Allium cepa L.

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