Cytogenetic studies on an epithelial cell line derived from poorly differentiated nasopharyngeal carcinoma

Abstract

An epithelial cell line, CNE-2, has been recently established from a poorly differentiated nasopharyngeal carcinoma, and it represents the first of its kind. Using chromosome banding techniques, cytogenetic analysis of the cell line was carried out. It was demonstrated that the chromosome numbers of the CNE-2 cells varied from 87 to 107 and the modal number was 104-103. All cells contained a series of structurally abnormal chromosomes, and most of them were either consistent or frequently found. Among these chromosomes there were two giant markers which, by banding pattern analysis, proved to be distinct from the so-called giant group A marker chromosomes previously found in many lymphoblastoid cell lines from NPC. Comparison between the CNE-2 and CNE, another epithelial cell line, which was established from well-differentiated squamous NPC, showed that while they were quite different in many cytogenetic aspects, they had three marker chromosomes in common, namely, an iso8q, a t(?;3q) and a small acrocentric one. The question of whether chromosome markers specific for NPC exist is discussed in the light of the data presented.

PARP1 Is Overexpressed in Nasopharyngeal Carcinoma and Its Inhibition Enhances Radiotherapy

“…Nasopharyngeal carcinoma cell lines C666-1 (13), CNE2 (14), HNE1 (15), and HONE1 (15) were obtained from nasopharyngeal carcinoma AoE Cell Line Repository and were propagated in RPMI-1640 (for C666-1) or Dulbecco's Modified Eagle Medium (DMEM; for other nasopharyngeal carcinoma cells) supplemented with 10% (v/v) FBS (Life Technologies) and 50 U/mL penicillin-streptomycin (Life Technologies). No authentication was carried out by the authors.…”

Variable Sensitivity of Endothelial Cells to Epirubicin in Xenografts of Human Nasopharyngeal Carcinoma CNE-2 Cells

“…s.c. xenografts of human nasopharyngeal carcinoma CNE-2, established from a nasopharyngeal carcinoma with WHO type III origin, 21 were passaged among BALB/c nude mice. Each group contained 6 mice.…”

Upregulation of GADD153 expression in the apoptotic signaling of N‐(4‐hydroxyphenyl)retinamide (4HPR)

“…In our study, we have adopted the macroarray cDNA-profiling approach to acquire the identities of the set of genes that might be modulated by 4HPR. Using a human epithelial cell line (CNE3) established from a poorly differentiated nasopharyngeal carcinoma, 13 we identified more than 20 genes whose expressions were downregulated in the presence of 4HPR, and only one gene, the growth arrest and DNA-damage inducible gene (gadd153) was upregulated. gadd153 encodes a 28 kDa protein that belongs to the C/EBP family of transcription factors.…”