Cytogenetic studies of five taxa of the tribe Attini (Formicidae: Myrmicinae)

Barros, Luísa Antônia Campos; Mariano, Cléa dos Santos Ferreira; Pompolo, Silvia das Graças

doi:10.1080/00087114.2013.780443

Cited by 16 publications

(20 citation statements)

References 22 publications

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“…C-banding was performed according to Sumner (1972) with minor adaptations as suggested by Barros et al (2013). Specific GC-and AT-rich regions were detected using sequential staining with the fluorochrome Cromomicin A 3 (CMA 3 ) and 4'6-diamidino-2-phenylindole (DAPI) following .…”

Section: Methodsmentioning

confidence: 99%

“…Studies concerning heterochromatin information are valuable in cytogenetic analysis of ants owing to the central role of heterochromatin in the chromosome evolution of the group (Imai et al, 1994). The cytogenetic data available for fungus-growing ants show heterochromatin distribution mainly at the centromeric and/or pericentromeric regions for species with low chromosome numbers n ≤ 12 (sensu Imai et al, 1988), such as Mycocepurus goeldii (Forel, 1893) (Barros et al, 2010), Trachymyrmex relictus Borgmeier, 1934(Barros et al, 2013, Trachymyrmex fuscus Emery, 1934(Barros et al, 2014a, and Atta spp. (Barros et al, 2014b(Barros et al, , 2015.…”

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Cytogenetic studies in Trachymyrmex holmgreni Wheeler, 1925 (Formicidae: Myrmicinae) by conventional and molecular methods

et al. 2018

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Over the past several decades, ant cytogenetic studies have focused on chromosome number and morphology; however, recently, additional information concerning heterochromatin composition and 45S rDNA location has become accessible. The fungus-growing ants are a peculiar ant group that cultivates fungus for food, and Trachymyrmex is suspected to be the sister group of leafcutter ants. Cytogenetic data are so far available for sixn Trachymyrmex species. The present study aimed to increase the knowledge about Trachymyrmex cytogenetics by the chromosomal characterization of Trachymyrmex holmgreni including the karyotyping, fluorochromes staining, 18S rDNA, and microsatellite (GA)15 fluorescence in situ hybridization (FISH). Karyotyped samples from four ant colonies showed 2n = 20 metacentric chromosomes. Centromeric heterochromatin rich in GC base pairs was detected in all chromosomes. FISH revealed the presence of rDNA clusters on the fourth chromosome pair, and an intense spreading of the microsatellite (GA)15 including exclusively euchromatic areas of the chromosomes. The GC-rich heterochromatin observed in different ant species may have a common origin and, thus, phylogenetic implication that needs to be further investigated. To the best of our knowledge, this study is the first report of the use of chromosomal physical location of repetitive DNA sequences by means of microsatellite probes in Formicidae.

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Section: Methodsmentioning

confidence: 99%

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Cytogenetic studies in Trachymyrmex holmgreni Wheeler, 1925 (Formicidae: Myrmicinae) by conventional and molecular methods

et al. 2018

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“…Karyotypes were organized using the Corel Photopaint X3® and Image Pro Plus® programs. The C-banding technique was performed according to Sumner (1972) with modifications proposed by Barros et al (2013). The GC and AT rich regions were detected using the fluorochromes Chromomycin A 3 (CMA 3 ) and 4'6-diamidin-2-phenylindole (DAPI) according to Schweizer (1980).…”

Section: Methodsmentioning

confidence: 99%

Banding Patterns of Three Leafcutter Ant Species of the GenusAtta(Formicidae: Myrmicinae) and Chromosomal Inferences

Barros¹,

Teixeira

Aguiar³

et al. 2014

Florida Entomologist

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“…The colonies and individuals analyzed are listed in Table 1. For the banding techniques, 4 to 10 individuals per species were used: C-banding for heterochromatin detection was performed according to Sumner (1972) with minor adaptations suggested by Barros et al (2013); sequential fluorochrome staining with CMA 3 /DA/DAPI (Schweizer 1980) was done to reveal specific GC- and AT-rich regions. To detect (NORs)nucleolus organizer regions in three species, 2 to 4 individuals of each species were studied using (FISH)fluorescence in situ hybridization with the 45S rDNA probe isolated from Arabidopsis thaliana (Moscone et al 1996).…”

Section: Methodsmentioning

confidence: 99%

“…Nowadays, ant cytogenetics is a rapidly developing research field (Delabie et al 2012). Cytogenetic data on fungus-growing ants with information for at least the chromosome number and morphology are available at present for 38 taxa (reviewed in Barros et al 2011, Cristiano et al 2013, Barros et al 2013, 2014a, 2014b, Cardoso et al 2014, Barros et al 2015), corresponding to about 10% of described species (Brandão et al 2011). In some ant genera, e.g.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): insights into chromosome evolution and taxonomic implications

Barros

Aguiar

et al. 2016

CCG

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Cytogenetic data for the genus Acromyrmex Mayr, 1865 are available, to date, for a few species from Brazil and Uruguay, which have uniform chromosome numbers (2n = 38). The recent cytogenetic data of Acromyrmex striatus (Roger, 1863), including its banding patterns, showed a distinct karyotype (2n = 22), similar to earlier studied Atta Fabricius, 1804 species. Karyological data are still scarce for the leafcutter ants and many gaps are still present for a proper understanding of this group. Therefore, this study aimed at increasing cytogenetic knowledge of the genus through the characterization of other six species: Acromyrmex balzani (Emery, 1890), Acromyrmex coronatus Fabricius, 1804, Acromyrmex disciger (Mayr, 1887), Acromyrmex echinatior (Forel, 1899), Acromyrmex niger (Smith, 1858) and Acromyrmex rugosus (Smith, 1858), all of which were collected in Minas Gerais – Brazil, except for Acromyrmex echinatior which was collected in Barro Colorado – Panama. The number and morphology of the chromosomes were studied and the following banding techniques were applied: C-banding, fluorochromes CMA3 and DAPI, as well as the detection of 45S rDNA using FISH technique. All the six species had the same chromosome number observed for already studied species, i.e. 2n = 38. Acromyrmex balzani had a different karyotype compared with other species mainly due to the first metacentric pair. The heterochromatin distribution also showed interspecific variation. Nevertheless, all the studied species had a pair of bands in the short arm of the first subtelocentric pair. The fluorochrome CMA3 visualized bands in the short arm of the first subtelocentric pair for all the six species, while Acromyrmex rugosus and Acromyrmex niger also demonstrated in the other chromosomes. The AT-rich regions with differential staining using DAPI were not observed. 45S ribosomal genes were identified by FISH in the short arm of the first subtelocentric pair in Acromyrmex coronatus, Acromyrmex disciger and Acromyrmex niger. The uniform chromosome number in the genus Acromyrmex (2n = 38) suggests that Acromyrmex striatus (2n = 22) should be transferred to a new genus. Other aspects of the chromosome evolution in ants are also discussed.

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Cytogenetic studies of five taxa of the tribe Attini (Formicidae: Myrmicinae)

Cited by 16 publications

References 22 publications

Cytogenetic studies in Trachymyrmex holmgreni Wheeler, 1925 (Formicidae: Myrmicinae) by conventional and molecular methods

Cytogenetic studies in Trachymyrmex holmgreni Wheeler, 1925 (Formicidae: Myrmicinae) by conventional and molecular methods

Banding Patterns of Three Leafcutter Ant Species of the GenusAtta(Formicidae: Myrmicinae) and Chromosomal Inferences

Cytogenetic data on six leafcutter ants of the genus Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): insights into chromosome evolution and taxonomic implications

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