Cytogenetic studies in mouse oocytes irradiated in vitro at different stages of maturation, by use of an early preantral follicle culture system

Jacquet, P.; Adriaens, I.; Buset, Jasmine; Neefs, Mieke; Vankerkom, Jean

doi:10.1016/j.mrgentox.2005.03.008

Cited by 29 publications

(18 citation statements)

References 28 publications

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“…MN in pre-embryos after irradiation of vitamin C treated mice study is consistent with the finding of other investigators regarding sensitivity of preovulatory stage to ionizing radiation for induction of chromosomal aberrations in mice (Reichert et al 1975, Tease & Fisher 1986, de Boer & van der Hoeven 1991, Jacquet et al 2005. Therefore, the increase of MN yield in pre-embryos might be explained in terms of DNA damages induced in cells forming the zygotes.…”

Section: Discussionsupporting

confidence: 91%

“…It is worthy to mention that several authors used the dose of 4 Gy for studying radiation-induced genomic instability (Konopacka & Rzeszowska-Wolny 1998), in vitro cytogenetic studies on human and mouse germ cells (Tusell et al 1995, Jacquet et al 2005 and prenatal effects of g-irradiation (Bang et al 2002).…”

Section: Parental (Male and Female) Irradiationmentioning

confidence: 99%

“…The majority of de novo structural chromosome aberrations in fetuses and newborns are considered being of paternal origin, that is of sperm origin (Olson & Magenis 1988). Cytogenetic investigations on oocytes irradiated with various doses of X-rays at the immature stage or at different stages of follicular growth on mouse have shown that the sensitivity of the oocyte to radiation induced chromosome aberrations can vary greatly according to the stage of oogenesis reached at the time of irradiation (Jacquet et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Cytogenetic damage in preimplantation mouse embryos generated after paternal and parental γ-irradiation and the influence of vitamin C

Mozdarani

Nazari²

2009

Reproduction

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Cytogenetic damage expressed as micronuclei (MN) in 4-8-cell embryos generated after irradiation of male or male and female mice in the absence and presence of vitamin C was investigated. Male NMRI mice were whole body exposed to 4 Gy g-rays and mated with non-irradiated superovulated female mice in 6 successive weeks after irradiation in a weekly interval. In experiments involving irradiation of both male and female mice, irradiated male mice for 6 weeks post irradiation were mated with female mice irradiated after induction of superovulation. Effect of 100 mg/kg vitamin C (ascorbic acid) on the frequency of MN was also studied. Pregnant animals were euthanized and embryos flushed from the oviducts and fixed on slides. The rate of MN observed in embryos generated from irradiated male compared with control group dramatically increased (P!0.01). Frequency of MN in this group decreased dramatically after vitamin C treatment (P!0.01). Frequency of MN in embryos generated by mating both male and female irradiated mice was higher than that observed for those embryos generated by irradiated male mice alone. However, a considerable modifying effect of vitamin C was observed for this group too (P!0.05). Results indicate that irradiation of gonads during spermatogenesis and preovulatory stage oocytes may lead to unstable chromosomal aberrations and probably stable chromosomal abnormalities affecting pairing and disjunction of chromosomes in successive preimplantation embryos expressed as MN. The way vitamin C reduces clastogenic effects of radiation on germ cells leading to reduced frequency of MN in pre-embryos might be due to its antioxidation and radical scavenging properties.

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Section: Discussionsupporting

confidence: 91%

Section: Parental (Male and Female) Irradiationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytogenetic damage in preimplantation mouse embryos generated after paternal and parental γ-irradiation and the influence of vitamin C

Mozdarani

Nazari²

2009

Reproduction

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“…As an example, when the parameters analyzed are chromosomal aberrations, DL and/or congenital malformations, studies have reported that oocyte radiation-sensitivity increased two-fold in the Chinese hamster, when compared to the mouse [34,35,61]. However, in the case of chromosome aberrations detected at MI/MII, the mature oocytes from guinea pig are more radio-sensitive than are mature mouse oocytes [23,79]. Despite this apparent disparity in results, depending on the species analyzed, it seems that a general trend can be outlined.…”

Section: Radiation-induced Genotoxic Effects In Mammalian Female Gmentioning

confidence: 99%

Radiobiology and Reproduction—What Can We Learn from Mammalian Females?

Ruiz‐Herrera

García

Garcia-Caldés

2012

Genes

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Ionizing radiation damages DNA and induces mutations as well as chromosomal reorganizations. Although radiotherapy increases survival among cancer patients, this treatment does not come without secondary effects, among which the most problematic is gonadal dysfunction, especially in women. Even more, if radio-induced DNA damage occurs in germ cells during spermatogenesis and/or oogenesis, they can produce chromosomal reorganizations associated with meiosis malfunction, abortions, as well as hereditary effects. However, most of our current knowledge of ionizing radiation genotoxic effects is derived from in vitro studies performed in somatic cells and there are only some experimental data that shed light on how germ cells work when affected by DNA alterations produced by ionizing radiation. In addition, these few data are often related to mammalian males, making it difficult to extrapolate the results to females. Here, we review the current knowledge of radiobiology and reproduction, paying attention to mammalian females. In order to do that, we will navigate across the female meiotic/reproductive cycle/life taking into account the radiation-induced genotoxic effects analysis and animal models used, published in recent decades.

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“…Studies in mouse oocytes have shown that radiation-induced DNA damage may cause chromosomal aberrations, such as aneuploidy, translocations, chromatid interchanges and breaks (Tease, 1983;Jacquet et al, 2005). Past studies hinted at the possibility of a limited DDR in fully grown oocytes (Mailhes et al, 1994;Bradshaw et al, 1995).…”

Section: Prophase To MI Transitionmentioning

confidence: 99%

The DNA Damage Response in Mammalian Oocytes.

Marangos

2012

Biology of Reproduction

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Cytogenetic studies in mouse oocytes irradiated in vitro at different stages of maturation, by use of an early preantral follicle culture system

Cited by 29 publications

References 28 publications

Cytogenetic damage in preimplantation mouse embryos generated after paternal and parental γ-irradiation and the influence of vitamin C

Cytogenetic damage in preimplantation mouse embryos generated after paternal and parental γ-irradiation and the influence of vitamin C

Radiobiology and Reproduction—What Can We Learn from Mammalian Females?

The DNA Damage Response in Mammalian Oocytes.

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