Cytogenetic status of healthy children assessed with the alkaline comet assay and the cytokinesis-block micronucleus cytome assay

Gajski, Goran; Gerić, Marko; Oreščanin, Višnja; Garaj‐Vrhovac, Vera

doi:10.1016/j.mrgentox.2012.09.011

Cited by 29 publications

(17 citation statements)

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“…Although it is mainly used in human cells both in vivo (ex vivo, in situ) and in vitro, the assay has its application in the evaluation of DNA damage in yeast [43][44][45], plant [46][47][48][49] and animal [29,[50][51][52][53] cells. Therefore, the comet assay has instantly found its application in different fields; from genetic and environmental toxicology to human epidemiology and biomonitoring [1,3,17,[54][55][56][57][58][59][60][61][62][63][64].…”

Section: Introductionmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

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Section: Introductionmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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“…In the study of 50 healthy children from urban areas in Croatia, ages from 414 years, girls showed higher mean values of the mean frequency of MN, mean frequency of nucleoplasmic bridges (NPBs), nuclear buds and the mean nuclear division index (NDI) compare to boys, but only total number of NPBs was statistically significant [5]. In the current study the mean frequency of MN for all subjects was 2.32±0.28 per 1000 BN cells which is higher value than the level obtained for the second age group.…”

Section: Discussionmentioning

confidence: 99%

“…Analysis of MN frequency in peripherals blood lymphocytes was conducted by cytokinesis-block technique-modification according to the Fenech and Morley [4,5,9].…”

Section: Variables and Participantsmentioning

confidence: 99%

“…Micronuclei (MN) are chromatin particles that are created as a result of chromosomal break or elimination of whole chromosomes as a consequence of mitotic spindle damage and a biomarker of genotoxic events [13] (and chromosomal instability [4]. Frequency of MN in peripheral blood lymphocytes of healthy subjects is environment specific [5] and increases with chronological age [613]. Specific increase of MN frequency with age was found in mice [14].…”

Section: Introduction mentioning

confidence: 99%

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Structure of Relations Between the Frequency of Micronuclei in Peripheral Blood Lymphocytes and Age, Gender, Smoking Habits and Socio-Demographic Factors in South-East Region of Serbia

et al. 2019

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Frequency of micronuclei (MN) in peripheral blood lymphocytes is a measure of genotoxicity and spontaneous chromosomal instability with many modifying factors. The aim of this work was to examine the relation between results of micronucleus test and set of socio-demographic parameters in healthy population in south-east Serbia using structure equation approach. Cytokinesis–block (CB) technique was used for analysis of MN frequency in peripheral blood lymphocytes on 133 healthy volunteers of various ages. Socio-demographic data was collected through a questionnaire. The mean frequency of MN gradually rose with age from 0.56±0.71 in new-borns to 5.48 ±3.65 in the 61-80 years age group (AG), with a decrease in the 81-92 years AG. MN frequency was positively correlated with age, altitude of birth place, altitude of place of residence, nuclear buds and nucleoplasmatic bridges, and negatively with education level and smoking habits. Linear structural model revealed age to be related to all of the examined variables, and indicates probable existence of another factor, independent of age, influencing all of these except nuclear buds. It can be concluded that the frequency of micronuclei is influenced by age and factor/s resembling socioeconomic status or lifestyle and this influence is independent of age.

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“…These confounding effects are minimized and even absent during childhood. However, monitoring of children populations may consider potentially genotoxic factors including indoor tobacco smoke, regional ozone level, airborne nanoparticles, food cont a m i n a n t ss u c ha sp e s t i c i d er e s i d u e sa n dc o m p o unds generated by cooking (Holland et al [84]), natural sources of ionizing and non-ionizing radiation, environmental pollutants, fuel and hydrocarbon emissions, which can vary significantly between rural or urban environmental settings [85].…”

Section: Dulout Et Al's Studymentioning

confidence: 99%

Genotoxic Risk in Human Populations Exposed to Pesticides

Aiassa¹

2018

Genotoxicity - A Predictable Risk to Our Actual World

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The importance of early detection of genetic damage is that it allows taking the necessary measures to reduce or suppress the exposure to the deleterious agent when it is still reversible, thus decreasing the risk of developing diseases. For this reason, genotoxicity tests should be considered as indispensable tools in the implementation of a complete medical surveillance in people potentially exposed to various environmental pollutants and especially those who live in the same place with people who have already developed some type of neoplasia at early ages in order to prevent the occurrence of tumors of environmental origin and work-related. On the other hand, the application of these tests is useful to detect possible long-term effects of substances that are introduced to the market without knowing exactly their capacity to affect human and environmental health.

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Cytogenetic status of healthy children assessed with the alkaline comet assay and the cytokinesis-block micronucleus cytome assay

Cited by 29 publications

References 71 publications

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Structure of Relations Between the Frequency of Micronuclei in Peripheral Blood Lymphocytes and Age, Gender, Smoking Habits and Socio-Demographic Factors in South-East Region of Serbia

Genotoxic Risk in Human Populations Exposed to Pesticides

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