Cytogenetic mapping in maize

Wang, Rachel; Chen, C.-C.

doi:10.1159/000082383

Cited by 11 publications

(6 citation statements)

References 114 publications

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“…With the advance of genome research, cytogenetic maps will not only be valuable for integrating and organizing genetic, molecular, and cytological information, but they will also provide a unique insight into genome organization in the context of the chromosomes. In plants, two general approaches have been adopted for construction of a cytogenetic map (for reviews, see Harper and Cande, 2000;Wang and Chen, 2005). The first general strategy, widely used in wheat (Triticum aestivum), barley (Hordeum vulgare), tomato (Lycopersicon esculentum), maize, and others, is to locate genetically mapped DNA markers to chromosomal segments relative to cytologically mapped breakpoints using cytogenetic stocks (McClintock, 1944;Khush and Rick, 1968;Weber and Helentjaris, 1989;Beckett, 1991;Werner et al, 1992;Coe et al, 1993;Lin et al, 1997;Kunzel et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Single-Copy Gene Fluorescence in Situ Hybridization and Its Use in the Construction of a Cytogenetic Map of Maize Chromosome 9

2006

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High-resolution cytogenetic maps provide important biological information on genome organization and function, as they correlate genetic distance with cytological structures, and are an invaluable complement to physical sequence data. The most direct way to generate a cytogenetic map is to localize genetically mapped genes onto chromosomes by fluorescence in situ hybridization (FISH). Detection of single-copy genes on plant chromosomes has been difficult. In this study, we developed a squash FISH procedure allowing successful detection of single-copy genes on maize (Zea mays) pachytene chromosomes. Using this method, the shortest probe that can be detected is 3.1 kb, and two sequences separated by ;100 kb can be resolved. To show the robust nature of this protocol, we localized nine genetically mapped single-copy genes on chromosome 9 in one FISH experiment. Integration of existing information from genetic maps and the BAC contigbased physical map with the cytological structure of chromosome 9 provides a comprehensive cross-referenced cytogenetic map and shows the dramatic reduction of recombination in the pericentromeric heterochromatic region. To establish a feasible mapping system for maize, we also developed a probe cocktail for unambiguous identification of the 10 maize pachytene chromosomes. These results provide a starting point toward constructing a high-resolution integrated cytogenetic map of maize.

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Section: Introductionmentioning

confidence: 99%

High-Resolution Single-Copy Gene Fluorescence in Situ Hybridization and Its Use in the Construction of a Cytogenetic Map of Maize Chromosome 9

2006

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“…For example, a 2.4-kb DNA target can be detected on maize somatic chromosomes (Danilova and Birchler 2008). However, single-copy sequences 3.1-kb at the least can be detected on maize pachytene chromosomes (Wang et al 2006a), even though pachytene chromosomes of maize are about 12 times longer than their mitotic metaphase counterparts (Wang and Chen 2005).…”

Section: Resultsmentioning

confidence: 99%

Higher axial-resolution and sensitivity pachytene fluorescence in situ hybridization protocol in tetraploid cotton

et al. 2009

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Fluorescence in situ hybridization (FISH) based on pachytene chromosomes has become an important cytogenetic tool to construct high axial-resolution and sensitivity cytogenetic maps. However, the application of this technique in cotton has lagged behind due to difficulties in chromosome preparation. To date, successful FISH based on cotton pachytene chromosomes has not been reported. In this study, the first protocol developed for pachytene chromosome preparation in tetraploid cotton is presented. This protocol yielded chromosome spreads suitable for large and small DNA probe FISH labeling. Two important parameters, axial-resolution and sensitivity, of FISH on mitotic metaphase and pachytene chromosomes were systematically analyzed. The results demonstrated that DNA targets separated by 0.6 cM and low-copy targets as small as 3-kb were resolved and detected, respectively, in pachytene FISH. The application of our FISH protocol will continue to improve and provide a point of departure for constructing an integrated high axial-resolution cytogenetic map in cotton.

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“…The present study has demonstrated the successful isolation of C 0 t-1 DNA from the B. napus genome. The C 0 t-1 DNA is routinely used as a blocking agent in in situ suppressive hybridization (Kahlem et al 2004;Dugan et al 2005;Wang and Chen 2005), so the isolated C 0 t-1 DNA of B. napus will be available for suppression hybridization of large B. napus genomic clones or genomic DNA in addition to chromosome banding.…”

Section: Discussionmentioning

confidence: 99%

Karyotyping of Brassica napus L. Based on C₀t‐1 DNA Banding by Fluorescence In Situ Hybridization

Wei

Wan-peng

Wang

et al. 2005

JIPB

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In order to precisely recognize and karyotype Brassica napus L. chromosomes, C 0 t-1 DNA was extracted from its genomic DNA, labeled with biotin-11-dUTP and in situ hybridized. The hybridized locations were detected by Cy3-conjugated streptavidin. Specific fluorescence in situ hybridization (FISH) signal bands were detected on all individual chromosome pairs. Each chromosome pair showed specific banding patterns. The B. napus karyotype has been constructed, for the first time, on the basis of both C 0 t-1 DNA FISH banding patterns and chromosome morphology. Key words:Brassica napus; C 0 t-1 DNA; chromosome banding; fluorescence in situ hybridization (FISH); karyotyping.Brassica napus L. (2n = 4x = 38; genome AACC), one of the most important oil crops in the world, is allotetraploid from spontaneous interspecific hybridization between putative diploid progenitors B. campestris L. (2n = 2x = 20; AA) and B. oleracea L. (2n = 2x = 18; CC) (U 1935). Recently, the multinational Brassica genome project was started to determine the complete sequence of the B. rapa L. A genome (Yang et al. 2005) and the B. oleracea C genome (Ayele et al. 2005). The sequences of the A genome from B. rapa and the C genome from B. oleracea will be certainly important for analyzing genome organization and localizing homologous genes in B. napus if corresponding chromosomes can be identified between the allotetraploid and the two diploids. Inevitably, chromosome identification and karyotype construction of B. napus are necessary basics for this to be undertaken. However, the chromosomes of B. napus are small, morphologically similar to each other, and quite numerous and, as such, the difficulties associated with precisely recognizing and karyotyping its chromosomes have never been resolved.The current karyotypes of B. napus were obtained on the basis of chromosome-staining methods, such as Giemsa staining, C-banding, chromomycin A3 (CMA3)/4',6'-diamidino-2-phenylindole (DAPI) fluorescent staining, silver staining, and fluorescence in situ hybridization (FISH) with rDNA (Olin-Fatih and these methods are invalid or non-fluorescent for identifying individual chromosomes of B. napus. Hence, new chromosome identification techniques need to be explored.The C 0 t-1 DNA is enriched with highly and moderately repetitive DNA sequences. In many plant species of economic importance, repeated sequences comprise a large proportion of the genome (Flavell et al. 1974; McCouch and Tanksley 1991). These DNA sequences distribute throughout the whole genome of eukaryotes. Hence, it is conceivable to band individual chromosomes of B. napus with C 0 t-1 DNA. The karyotyping results presented in the present study are a combination of a morphometric study and FISH with a C 0 t-1 DNA probe to B. napus chromosomes, which allowed more effective karyotype construction for this allopolyploid Brassica species. Materials and Methods Plant materialsBrassica napus L. cv. Zhongyou 821, developed by the Institute of Oil Crops, Chinese Academy of Agricultural Sciences, was used...

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Cytogenetic mapping in maize

Cited by 11 publications

References 114 publications

High-Resolution Single-Copy Gene Fluorescence in Situ Hybridization and Its Use in the Construction of a Cytogenetic Map of Maize Chromosome 9

High-Resolution Single-Copy Gene Fluorescence in Situ Hybridization and Its Use in the Construction of a Cytogenetic Map of Maize Chromosome 9

Higher axial-resolution and sensitivity pachytene fluorescence in situ hybridization protocol in tetraploid cotton

Karyotyping of Brassica napus L. Based on C₀t‐1 DNA Banding by Fluorescence In Situ Hybridization

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Cytogenetic mapping in maize

Cited by 11 publications

References 114 publications

High-Resolution Single-Copy Gene Fluorescence in Situ Hybridization and Its Use in the Construction of a Cytogenetic Map of Maize Chromosome 9

High-Resolution Single-Copy Gene Fluorescence in Situ Hybridization and Its Use in the Construction of a Cytogenetic Map of Maize Chromosome 9

Higher axial-resolution and sensitivity pachytene fluorescence in situ hybridization protocol in tetraploid cotton

Karyotyping of Brassica napus L. Based on C0t‐1 DNA Banding by Fluorescence In Situ Hybridization

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Karyotyping of Brassica napus L. Based on C₀t‐1 DNA Banding by Fluorescence In Situ Hybridization