Cytogenetic genotoxicity of amoxicillin

İstifli, Erman Salih; Topaktaş, Mehmet

doi:10.1002/em.20531

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…This is also an opportunity to compare the genotoxic risk of DRP in in vivo animals or in another different test system. Previous studies have been shown that β-lactam antibiotics had no genotoxic and cytotoxic effects on human peripheral lymphocytes (19,(45)(46)(47). Likewise, we observed similar results in the present study and the results suggested that DRP caused mutations only at doses much higher than the therapeutic doses.…”

Section: Discussionsupporting

confidence: 92%

Investigation of genotoxic effects of doripenem using cytogenetic and molecular methods

Aydın

Rencüzoğulları

Bozkurt

et al. 2017

Cell Mol Biol (Noisy-le-grand)

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The main aim of this study was to investigate the genotoxic effects of doripenem (DRP) using both cytogenetic and molecular test systems. Although there have been some studies reporting the effects of DRP, none of them has shown the genotoxic effects of DRP. In order to achieve the main aim of the study, the human peripheral lymphocytes were treated with 100 μg/ml, 200 μg/ml, and 400 μg/ml concentrations of DRP for 24 and 48 hours, and the chromosome aberration (CA) and micronucleus (MN) methods were used as the cytogenetic tests and RAPD-PCR method was used as the molecular test to determine the genotoxic effects of DRP. DRP did not induce the chromosome aberrations and micronucleus frequencies at all concentrations and at all treatment periods. So, it was concluded that DRP did not show any cytotoxic effect. However, DRP increased the number of polymorphic bands and decreased the ratio of genomic template stability, especially at the 48-hour treatment period. In this study, according to the obtained results, it was determined that DRP failed to show any genotoxic risk at the therapeutic doses. This result also indicates that DRP could be a reliable antibiotics according to its rapid metabolism.

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Section: Discussionsupporting

confidence: 92%

Investigation of genotoxic effects of doripenem using cytogenetic and molecular methods

Aydın

Rencüzoğulları

Bozkurt

et al. 2017

Cell Mol Biol (Noisy-le-grand)

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“…Using the sisters chromatid exchange method, mutagenetic and carcinogenic potentials of different substances were examinated [16,17,[20][21][22][23][24][25]. Antibiotics and non-steroidal anti-inflammatory drugs (NSAIDs) were just some of the most investigated subjects.…”

Section: Discussionmentioning

confidence: 99%

“…Various studies in vitro and vivo deal with different results and conclusions. Xie et al [21] suggest that Tetracycline induce significant increases in SCE even at the lowest concentrations, while, according to Istifli & Topaktaş [20] and Jaju et al [22], Amoxicillin does not behave genotoxic, with recommendation for safe usage in cases of bacterial infections. Also the metabolites of Amoxicillin do not have a significant effect on cell proliferation [20].…”

Section: Discussionmentioning

confidence: 99%

“…Xie et al [21] suggest that Tetracycline induce significant increases in SCE even at the lowest concentrations, while, according to Istifli & Topaktaş [20] and Jaju et al [22], Amoxicillin does not behave genotoxic, with recommendation for safe usage in cases of bacterial infections. Also the metabolites of Amoxicillin do not have a significant effect on cell proliferation [20]. Benzathine penicillin G (BPG) might also be considered as the "safe" antibiotic for a short-term ex posure [23], although in cases of a long-time exposure, SCE frequency changes might be expected [24].…”

Section: Discussionmentioning

confidence: 99%

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Is there a connection between synthetic bone grafts and sisters chromatide exchange?

Köseoğlu¹,

Brkić²,

Erdem³

et al. 2013

OJST

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Background: In oral and maxillofacial surgery, synthetic bone grafts are most widely used as bone substitutes, due to the limited sources of autologous bone. The aim of this study was to examine the influence of three different synthetic bone grafts (Cerasorb, Fortoss and Perioglass) on sisters chromatide exchanges (SCEs) in peripheral lymphocytes. Materials and Methods: Peripheral blood samples taken from 68 patients (45 females and 23 males), who underwent oral surgery procedures, such as apical resection, cyst enucleation or periodontal curretage, were obtained for SCE a day before and two months after the surgeries. A control group included 30 patients, while the study group was made of the patients who underwent bone grafting with Cerasorb ® (11 patients), Fortoss ® VITAL (10 patients) or Perioglass ® (17 patients). Results: Comparing with the results of the study group before and after the treatment, it was concluded that the results were statistically significant (p = 0.001). In the Perioglass ® subgroup, a greater statistical significance (p = 0.003) was noted, than that in either the Cerasorb ® (p = 0.620) or Fortoss ® (p = 0.210) subgroups, in which there was no statistical significance. Conclusions: Although further investigations may be necessary, our results suggest that the synthetic bone grafts might have an influence on SCE in peripheral lymphocytes.

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“…Para explicar la no inducción de ICHs luego del tratamiento con ivermectina Estos resultados concuerdan con los observados para otros compuestos, como es el caso del antibiótico amoxicilina, que no incrementa la frecuencia de ICHs en linfocitos humanos cultivados in vitro en presencia o ausencia de un sistema de activación metabólica (Istifli, E.S. y Topaktaş, M., 2010), así como el insecticida propargite en células vegetales in vivo luego del tratamiento con los fungicidas fenarimol e iprodiona (Gadeva, P. y Dimitrov, B., 2008).…”

Section: Análisis Estadísticounclassified

Ivermectinas: evaluación de su efecto deletéreo mediante ensayos de genotoxicidad

Molinari

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El propósito que animó a la realización del presente trabajo de Tesis Doctoral fue analizar el efecto deletéreo ejercido por el antiparasitario antihelmíntico ivermectina así como también una de sus formulaciones comerciales más utilizadas en nuestro país, Ivomec® (1% ivermectina, Merial S.A., Buenos Aires, Argentina). Dicho efecto fue evaluado in vitro en cultivos celulares de células mamíferos (CHO-K1) e insectos (CCL-126) así como en cultivos linfocitarios humanos mediante diferentes estimadores de genotoxicidad y citotoxicidad. Los resultados mostraron que ninguno de los compuestos analizados indujo un incremento significativo en la frecuencia de intercambios de cromátidas hermanas en células de las líneas celulares empleadas ni en los cultivos linfocitarios humanos. De manera similar, ni la ivermectina ni el Ivomec® resultaron ser inductores de micronúcleos en células CHO-K1 y no modificaron el índice de división nuclear. Sin embargo, tanto ivermectina como Ivomec® promovieron la formación de rupturas de cadena simple en el ADN de células CHO-K1 y CCL-126. Dichas células resultaron ser eficientes en la reparación del daño previamente inducido por estos compuestos evidenciado mediante la metodología del ensayo cometa. Por otro lado, ambos compuestos, indujeron marcadas alteraciones en la cinética de proliferación celular e índice mitótico tanto en células correspondientes a líneas celulares establecidas como en linfocitos humanos. Finalmente, una marcada citotoxicidad fue puesta en evidencia por ensayos colorimétricos específicos mostrando variaciones que dependieron tanto del tipo celular como de los sistemas de cultivo empleados. En líneas generales, los resultados obtenidos pondrían en evidencia que la ivermectina presenta un patrón de daño similar al ejercido por Ivomec® a igual concentración. Nuestros resultados permiten inferir que el efecto deletéreo producido por Ivomec® estaría producido por el principio activo presente en su formulación pudiéndose descartar la posibilidad de la presencia de algún otro agente(s) inductor(es) de daño en la composición de la misma. Asimismo, estos resultados demuestran que la ivermectina presenta la capacidad de inducir daño en la molécula de ADN, al menos de células CHO-K1 y CCL-126, y además, manifestar un efecto altamente citotóxico. De este modo, su uso masivo pondría en serio peligro a los organismos que habitualmente son tratados con ivermectina entre los cuales se encuentran, sin lugar a dudas, los seres humanos. Por lo expuesto, se hace evidente la necesidad de evaluación en un futuro cercano en lo referente a la peligrosidad de la ivermectina por parte de la Agencia para la Protección del Medioambiente en los Estados Unidos de Norteamérica (EPA), institución encargada de brindar los lineamientos acerca de la regulación sobre su uso con el fin de minimizar el riesgo para todos los organismos expuestos directa o indirectamente a este tipo de agente terapéutico.

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Cytogenetic genotoxicity of amoxicillin

Cited by 9 publications

References 27 publications

Investigation of genotoxic effects of doripenem using cytogenetic and molecular methods

Investigation of genotoxic effects of doripenem using cytogenetic and molecular methods

Is there a connection between synthetic bone grafts and sisters chromatide exchange?

Ivermectinas: evaluación de su efecto deletéreo mediante ensayos de genotoxicidad

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