Cytogenetic Fine Structure and Chromosomal Localization of the White Gene in Drosophila melanogaster

Sorsa, Veikko; Green, M. M.; Beermann, Wolfgang

doi:10.1038/newbio245034a0

Cited by 58 publications

(19 citation statements)

References 7 publications

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“…To coordinate the physical and genetic maps, we have determined by in situ hybridization the positions, within the cloned interval, of the breakpoints of a series of chromosomal rearrangements. These breakpoints have previously been shown to bracket the white locus by genetic analysis (6,20,21) and to bracket the sequences of the 3.1-kb BamHI fragment (coordinates + 1.6 to +4.7) (7). (For a more detailed discussion of the rearrangements used, see ref.…”

Section: Resultsmentioning

confidence: 99%

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Physical map of the white locus of Drosophila melanogaster.

Levis¹,

Bingham²,

Rubin³

1982

Proc. Natl. Acad. Sci. U.S.A.

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The white locus of Drosophila melanogaster is a genetically well-characterized locus, mutations in which alter the degree or pattern of pigmentation of the eyes. Using a previously cloned DNA segment containing a portion of the white locus of a mutant allele, we have cloned and characterized the DNA of a 48-kilobase chromosomal region ofthe Canton S wild-type strain. We have mapped the positions, relative to restriction endonuclease cleavage sites, of several previously characterized chromosomal rearrangement breakpoints that bracket the white locus. These results define a segment of 14 kilobases that contains all of the white locus sequences necessary for the production of a wild-type eye color phenotype. By conventional criteria, no repetitive sequences are present within this 14-kilobase segment; however, we have identified an extremely weak DNA sequence homology between a portion of this segment and a chromosomal region in the vicinity of the zeste locus.The wl mutation at the white (w) locus was the first mutation reported in Drosophila melanogaster (1) We have previously isolated, by recombinant DNA techniques, a single-copy DNA segment from the white locus of a strain carrying the white-apricot (wa) allele (7). In this report, we describe the molecular cloning of overlapping segments of the Canton S wild-type white locus region. This has allowed us to construct a physical map ofrestriction enzyme cleavage sites in this 48-kilobase (kb) segment, to orient this physical map with respect to the genetic map, and to define a segment of 14 kb that contains all ofthe white locus sequences necessary to allow the production of a w+ (wild-type eye color) phenotype.MATERLALS AND METHODS Drosophila Strains and DNA Preparation. The Drosophila strains used have been described (7). DNA was prepared from adult flies that were anesthetized with C02, collected on ice, rapidly frozen, and stored at -700C. Flies were disrupted and a crude nuclear pellet was prepared as described (8) except that all steps subsequent to grinding were done on ice and Nitex 44 filters were used in place of milk filters. The nuclei were suspended in a small volume of homogenization buffer to which was added 10 vol of 30 mM Tris'HCl, pH 8.0/100 mM EDTA containing proteinase K at 0.5 mg/ml followed by N-lauroylsarcosine to 1%. After incubation overnight at 370C, the DNA was banded in CsCl gradients (9).Recombinant Phage and DNA Preparation. Phage from a Charon 4/Canton S D. melanogaster library (10) were screened by plaque hybridization (11) at a density of 2 x 104 plaques per 24 X 24 cm agar plate as described (7). For DNA preparation, phage were grown in 200 ml of liquid culture (7), debris was removed by low-speed centrifugation, and the phage were precipitated with polyethylene glycol (12), suspended in 2 ml of 10 mM Tris HCl, pH 7.5/10 mM MgSO4 containing 20 ,ug of DNase (Sigma), extracted with an equal volume of CHC13, and purified by two cycles ofCsCl step-gradient centrifugation (13). DNA was isolated from purified phage by phenol ...

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Section: Resultsmentioning

confidence: 99%

“…) In(l)z+4b9 inverts a large chromosomal segment beginning near the proximal end of the white locus in 3C and extending toward the centromere to subdivision 12BC (21). The whiteassociated breakpoint of the inversion falls between white and roughest, the closest known complementation group toward the centromere from white (21). The DNA of Ami.…”

Section: Resultsmentioning

confidence: 99%

Physical map of the white locus of Drosophila melanogaster.

Levis¹,

Bingham²,

Rubin³

1982

Proc. Natl. Acad. Sci. U.S.A.

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“…Other questions concerned DNA replication in polyene chromosomes (Arcos-Teran and Beermann 1968) and he demonstrated that chromatids from diploid cells persist until high levels of polyteny have been attained (Beermann and Pelling 1965). Beermann was involved in ultrastructural studies of polytene chromosomes (Beermann and Bahr 1954) and in the fine structure analysis of the white locus in Drosophila (Sorsa et al 1973). Beermann has critically discussed ideas about chromosome structure, in particular with regard to the organisation of polytene chromosomes (Beermann 1972).…”

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confidence: 98%

In memoriam: Wolfgang Beermann

Hennig¹

2001

Chromosoma

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“…The uncertainty in designating particular, sub-light microscopic bands as gene loci is best exemplified by the work of Sorsa, Green, andBeerman (1973) at "white" andWelshons (1974) at "Notch".…”

Section: Rna Processingmentioning

confidence: 99%

“…More modern electron microscope mapping techniques (Sorsa and Sorsa, 1967) have recently been employed to attain greater resolution. Sorsa, Green, and Beerman (1973) recently employed these techniques in a cytogenetic study of white. Prior to this study, the white locus has been variously assigned to band 3C2 or the doublet 3C2-3 on the basis of rearrangement breakpoint determination and deletion mapping (see Lefevre and Green, 1972).…”

Section: Gene-band Assignmentmentioning

confidence: 99%

An analysis of the Notch locus in Drosophila melanogaster

Welter

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Cytogenetic Fine Structure and Chromosomal Localization of the White Gene in Drosophila melanogaster

Cited by 58 publications

References 7 publications

Physical map of the white locus of Drosophila melanogaster.

Physical map of the white locus of Drosophila melanogaster.

In memoriam: Wolfgang Beermann

An analysis of the Notch locus in Drosophila melanogaster

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