Cytogenetic effects of recombinant interferon-γ on lymphocytes cultures from patients with non–small cell lung cancer

Baka, Sofia; Ekonomopoulou, Maria T.; Kosmidis, Christoforos; Efthimiadis, Christoforos; Iakovidou‐Kritsi, Zafiroula

doi:10.1016/j.cancergencyto.2009.04.004

Cited by 5 publications

(4 citation statements)

References 37 publications

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“…These data were similar to the findings from earlier studies (Varga et al, 2011). PI has been used for the assessment of the cytostatic action of various therapeutic agents (Baka et al, 2009). C. islandica was useful and increased the PI, which was decreased by diabetes.…”

Section: Discussionsupporting

confidence: 90%

The ameliorative effect ofCetraria islandicaagainst diabetes-induced genetic and oxidative damage in human blood

Çolak

Geyikoğlu

Türkez

et al. 2013

Pharmaceutical Biology

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Context: The aqueous extracts of Cetraria islandica (L.) Ach. (Parmeliaceae) is traditionally used in many countries against a number of conditions, including inflammatory conditions. Objective: The present study aimed to assess, for the first time, the effectiveness of C. islandica in cultured primary blood cells of Type 1 diabetes subjects. Materials and methods: Diabetic and control blood samples were treated with or without aqueous lichen extract (5 and 10 mg mL À1) for 48 h. The activity of antioxidant enzymes in erythrocytes and also malondialdehyde levels in plasma were determined to evaluate the oxidative status. DNA damages were analyzed by SCE, MN and comet assays in cultured human lymphocytes. Additionally, proliferation index (PI) was evaluated in peripheral blood lymphocytes. Results: There were significant increases in observed total DNA damage (comet assay) (240.2%) and SCE (168.8%), but not in MN frequencies of cultures with diabetes as compared (p40.05) to controls. Whereas, the significant reductions of total DNA damage (69.2 and 65.3%) and SCE frequencies (17.7 and 12.3%) were determined when the 5 and 10 mg mL À1 lichen extract was added to the cell culture medium, respectively. However, lichen extract did not completely inhibit the induction of SCEs in lymphocytes of patients with diabetes. C. islandica extract was also useful on PI rates. Discussion: In conclusion, the antioxidant role of C. islandica in alleviating diabetes-induced genomic instability and for increasing cell viability was firstly indicated in the present study.

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Section: Discussionsupporting

confidence: 90%

The ameliorative effect ofCetraria islandicaagainst diabetes-induced genetic and oxidative damage in human blood

Çolak

Geyikoğlu

Türkez

et al. 2013

Pharmaceutical Biology

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“…The effects of IFN alone or combined with other treatment modalities (RT and CT) in NSCLC have been investigated. Arpin et al reported that IFN-γ did not display cytotoxic activity and that it may actually induce repair mechanisms (31). We found that IFN-γ levels significantly decreased following CRT only in GBM.…”

Section: Discussionmentioning

confidence: 44%

Prognostic value of interferon-gamma, interleukin-6, and tumor necrosis factor-alpha in the radiation response of patients diagnosed with locally advanced non-small-cell lung cancer and glioblastoma multiforme

Deniz¹,

Gürbîlek²,

Koç³

2018

Turk J Med Sci

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Background/aim: This study aimed to investigate the effect of chemoradiotherapy (CRT) on interferon-gamma (IFN-γ), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), which are critical markers of the clinical radiation response of patients with locally advanced non-small-cell lung cancer (NSCLC) and glioblastoma multiforme (GBM). Materials and methods: Thirty patients who were treated with CRT and 20 healthy controls were prospectively evaluated. Circulating levels of cytokines were measured by enzyme-linked immunosorbent assay procedure. Post-CRT and pre-CRT levels were compared. Results: Post-CRT, TNF-α and IFN-γ levels were significantly lower than pre-CRT levels in the NSCLC and GBM groups, respectively. The statistical analysis did not show any significant difference between the post- and pre-CRT IL-6 levels. However, the pre-CRT IL-6 levels in the GBM group and post-CRT IL-6 levels in the NSCLC group were significantly higher than those of the control group. Conclusion: CRT affected TNF-α levels in NSCLC and IFN-γ levels in GBM, with the levels of both decreasing significantly. The IL-6 levels of the post-CRT NSCLC group were higher than those of the post-CRT GBM group. Irradiation-induced IL-6 may be responsible for tumor regrowth. Therefore, treatment with IL-6 inhibitors could be a potential therapeutic strategy for sensitizing NSCLC to irradiation in the clinic.

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“…The question that then arises is how IFN␥ negatively regulates NP proliferation. One possibility is that IFN␥ induces cell-cycle arrest, an action that has been demonstrated previously in carcinoma cells (Burke et al, 1997;Shyu et al, 2000;Vivo et al, 2001;Baka et al, 2009) and mammalian epithelial cells (Harvat and Jetten, 1996). The anti-proliferative action in these cells is mainly regulated via activation of signal transducer and activator of transcription 1 (STAT1) and downregulation of cyclin A and cyclin E expression (Kortylewski et al, 2004).…”

Section: Discussionmentioning

confidence: 80%

Endogenous Interferon Directly Regulates Neural Precursors in the Non-Inflammatory Brain

Li¹,

Walker²,

Zhang³

et al. 2010

Journal of Neuroscience

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Although a number of growth factors have been shown to be involved in neurogenesis, the role of inflammatory cytokines remains relatively unexplored in the normal brain. Here we investigated the effect of interferon gamma (IFN␥) in the regulation of neural precursor (NP) activity in both the developing and the adult mouse brain. Exogenous IFN␥ inhibited neurosphere formation from the wild-type neonatal and adult subventricular zone (SVZ). More importantly, however, these effects were mirrored in vivo, with mutant mice lacking endogenous IFN␥ displaying enhanced neurogenesis, as demonstrated by an increase in proliferative bromodeoxyuridinelabeled cells in the SVZ and an increased percentage of newborn neurons in the olfactory bulb. Furthermore, NPs isolated from IFN␥ null mice exhibited an increase in self-renewal ability and in the capacity to produce differentiated neurons and oligodendrocytes. These effects resulted from the direct action of IFN␥ on the NPs, as determined by single-cell assays and the fact that nearly all the neurospheres were derived from cells positive for major histocompatibility complex class I antigen, a downstream marker of IFN␥-mediated activation. Moreover, the inhibitory effect was ameliorated in the presence of SVZ-derived microglia, with their removal resulting in almost complete inhibition of NP proliferation. Interestingly, in contrast to the results obtained in the adult, exogenous IFN␥ treatment stimulated neurosphere formation from the embryonic brain, an effect that was mediated by sonic hedgehog. Together these findings provide the first direct evidence that IFN␥ acts as a regulator of the active NP pool in the non-inflammatory brain.

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Cytogenetic effects of recombinant interferon-γ on lymphocytes cultures from patients with non–small cell lung cancer

Cited by 5 publications

References 37 publications

The ameliorative effect ofCetraria islandicaagainst diabetes-induced genetic and oxidative damage in human blood

The ameliorative effect ofCetraria islandicaagainst diabetes-induced genetic and oxidative damage in human blood

Prognostic value of interferon-gamma, interleukin-6, and tumor necrosis factor-alpha in the radiation response of patients diagnosed with locally advanced non-small-cell lung cancer and glioblastoma multiforme

Endogenous Interferon Directly Regulates Neural Precursors in the Non-Inflammatory Brain

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