Cytogenetic effects of nine Helichrysum taxa in human lymphocytes culture

Eroğlu, Halil Erhan; Aksoy, Ahmet; Hamzaoğlu, Ergin; Budak, Ümit; Albayrak, Sevil

doi:10.1007/s10616-009-9193-0

Cited by 30 publications

(32 citation statements)

References 38 publications

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“…Considering cultures with co-treatment, our results revealed that the extracts of T. chamaedrys at concentrations of 500 and 1,000 lg/ml and T. montanum at all tested concentrations (125-1,000 lg/ml) significantly decreased the NDI compared to the MMC-treated cells alone; this decrease in cell proliferation may be explained by permitting the repair of MMC induced DNA damages. Eroglu et al (2009) considered that by permitting repair of genotoxic lesions, mitotic delay may have changed the frequency of cells with two or more nuclei at the time of harvest by changing the number of cells that underwent mitosis.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of genotoxicity and antimutagenicity of methanolic extracts from Teucrium chamaedrys and Teucrium montanum in human lymphocytes using micronucleus assay

et al. 2013

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Since Teucrium chamaedrys and Teucrium montanum are the most popular plants used in the treatment of many diseases, we evaluated genotoxic potential of their methanolic extracts on cultured human peripheral blood lymphocytes (PBLs) using cytokinesis-block micronucleus (MN) assay. Cultures were treated with four concentrations of both plants (125, 250, 500 and 1,000 lg/ml), both separately and in combination with mitomycin C (MMC). The results revealed that extract of T. chamaedrys administered at the tested concentrations did not significantly affect the mean MN frequency in comparison to untreated cells. Methanolic extract of T. montanum increased the mean MN frequency in PBL at the tested concentrations, but significantly only at the concentration of 1,000 lg/ml. In all tested concentrations, the extract of T. chamaedrys significantly reduced the MMC-induced MN frequency, in a dose dependent manner (r = -0.687, p \ 0.01). The extract of T. montanum decreased the MMC-induced MN frequency at the tested concentrations, but statistically only at 125 lg/ ml. Both extracts administered alone did not significantly affect the nuclear division index (NDI) at the tested concentrations. In the combined treatments with MMC, the extract obtained from T. chamaedrys in the concentrations of 500 and 1,000 lg/ml significantly decreased NDI values in comparison to MMC-treated cells alone, while the extract of T. montanum significantly decreased NDI at all tested concentrations. Both extracts nonsignificantly decreased NDI at all tested concentrations in comparison to untreated cells. Our results suggest the important function of T. chamaedrys extract in cancer therapy, this methanolic extract may prevent genotoxic effects of chemotherapy in PBLs.

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Section: Discussionmentioning

confidence: 99%

Comparative study of genotoxicity and antimutagenicity of methanolic extracts from Teucrium chamaedrys and Teucrium montanum in human lymphocytes using micronucleus assay

et al. 2013

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“…H. arenarium, an infusion of the bright yellow flowers, is used in the treatment of gallbladder disorders and as a diuretic in treating rheumatism and cystitis. It is a component in zahraa, an herbal tea used for medicinal purposes in some countries [20]. A. sativum and T. parthenium have a broad spectrum of biological activity.…”

Section: Introductionmentioning

confidence: 99%

Investigations on the Effects of Five Different Plant Extracts on the Two-Spotted MiteTetranychus urticaeKoch (Arachnida: Tetranychidae)

Erdoğan

Yıldırım

Sever

2012

Psyche: A Journal of Entomology

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Two-spotted mite, Tetranychus urticae Koch (Arac.: Tetranychidae), is an economic pest worldwide including Turkey, causing serious damage to vegetables, flowers, and fruit crops. In recent years, broad-spectrum insecticides/miticides have been used to control this pest in Turkey. Control is difficult mainly due to resistance to conventional pesticides. This study was conducted to determine efficacy of pesticides extracted from five different plants [i.e., Allium sativum L. (Amaryllidaceae), Rhododendron luteum S. (Ericaceae), Helichrysum arenarium L. (Asteraceae), Veratrum album L. (Liliaceae), and Tanacetum parthenium L. (Asteraceae)] against this mite. Bioassays were tested by two different methods to determine the effects of varying concentrations. Experiments were performed using 3 cm diameter leaf disk from unsprayed bean plants (Phaseolus vulgaris L.). In addition, the effects of the extracts on reproduction and oviposition were investigated. The extract yielded high mortality. In the lowest-concentration bioassays, the adult mites laid lower numbers of eggs compared to the untreated control. No ovicidal effect was observed.

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“…This fixation step was repeated three times. Slides were air-dried and stained with giemsa (Eroglu et al 2009). …”

Section: Chemicalsmentioning

confidence: 99%

“…The MN staining was performed according to Eroglu et al (2009). The slides were scored by a single observer.…”

Section: Staining and Examination Of Micronucleusmentioning

confidence: 99%

In vitro cytotoxic potential of newly synthesized furo[3,2-c]pyran-4-one derivatives in cultured human lymphocytes

2011

Self Cite

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The in vitro cytotoxic potentials of Furo[3,2-c]pyran-4-one derivatives in human lymphocytes were investigated. Blood samples were obtained from six healthy donors, non-smoking volunteers, which were incubated and exposed to increasing concentrations (0.05, 0.1, 0.5, 1 and 2 mg/mL) of Furo[3,2-c] pyran-4-one derivatives which are methyl 2-methoxy-7-(4-methylbenzoyl)-6-(4-methylphenyl)-4-oxo-4H-furo [3,2-c]pyran-3-carboxylate (1a) and methyl 2-methoxy-7-(4-methoxybenzoyl)-6-(4-methoxyphenyl)-4-oxo-4H-furo[3,2-c]pyran-3-carboxylate (1b). Compounds 1a and 1b induced micronucleus, mitotic and replication indexes in human lymphocytes (1 and 2 mg/mL). The increases of micronucleus, mitotic and replication indexes show that compounds at high concentrations may become cytotoxic, genotoxic and carcinogenic.

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Cytogenetic effects of nine Helichrysum taxa in human lymphocytes culture

Cited by 30 publications

References 38 publications

Comparative study of genotoxicity and antimutagenicity of methanolic extracts from Teucrium chamaedrys and Teucrium montanum in human lymphocytes using micronucleus assay

Comparative study of genotoxicity and antimutagenicity of methanolic extracts from Teucrium chamaedrys and Teucrium montanum in human lymphocytes using micronucleus assay

Investigations on the Effects of Five Different Plant Extracts on the Two-Spotted MiteTetranychus urticaeKoch (Arachnida: Tetranychidae)

In vitro cytotoxic potential of newly synthesized furo[3,2-c]pyran-4-one derivatives in cultured human lymphocytes

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