Cytogenetic effects of combined radioactive (137Cs) and chemical (Cd, Pb, and 2,4-D herbicide) contamination on spring barley intercalar meristem cells

Гераськин, С. А.; Kim, Jin Kyu; Dikarev, Vladimir G.; Oudalova, A.; Dikareva, Nina S.; Spirin, Yevgeniy V.

doi:10.1016/j.mrgentox.2005.06.004

Cited by 31 publications

(23 citation statements)

References 28 publications

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“…Thus, MI changes were not proportional to increase in metal content in the solution. Similar phenomena were observes for other metals, for example, Cd [32,33].…”

Section: Introductionsupporting

confidence: 84%

CYTOGENETIC EFFECTS IN Allium сepa L. RESULTED FROM SEPARATE AND COMBINED EXPOSURE TO Cu, Zn and Ni

Ulyanenko¹,

Reva²,

Synzynys³

2017

S-h. biol.

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A b s t r a c tHeavy metal contaminations of agricultural lands necessitate the study of phyto-and genotoxic effects in plants of different types. The impact of elevated concentrations of biologically essential metals, e.g. Cu and Zn, and the metals with a pronounced toxic effect even at low concentrations is of special importance. Generally, the model objects (e.g. Allium cepa L.) are used to simulate the impact of one of the metals. In this case the concentration chosen is greater (100 times or more) than the levels of actual contamination and maximum permissible concentrations in different environments. Data on the combined action of metals at concentrations actually existing in the environment are practically not reported, which prevents the development of standards to limit their impact on ecosystems and agrobiocenoses. Our objective was to compare the cytogenetic changes in the root meristem of Allium cepa exposed to different concentrations of Cu, Zn and Ni separately and combinedly. Experiments were carried out in 4-fold replication. The roots of onion plants (10 per replication) were germinated in distilled water (control) or Cu(NO 3 ) 2 ∕3H 2 O, Zn(NO 3 ) 2 ∕6H 2 O and Ni(NO 3 ) 2 ∕6H 2 O solutions. Salt concentrations corresponded to the maximum permissible concentrations in water for fish-farming (Сu -0.001 mg/l, Ni and Zn -0.01 мg/l) and for household purposes (Сu and Zn -1.0 mg/l, Ni -0.02 mg/l). Chromosome aberrations were viewed in 180-790 cells of onion root tips in fresh crush preparations after acetic-orcein staining. We estimated the mitotic index calculated as the fraction of mitotic cells to the total number of cells in the root meristem, the frequency of aberrant cells, and the types of chromosomal aberrations. The influence of each element and their various combinations on cytogenetic parameters was compared that allowed us to determine the coefficient of antagonism. It has been shown that Cu, Zn and Ni ions, as depending on their properties, inhibited cell division in onion root meristem to varying degrees. At relatively low concentrations of metals, equal to the MPC in water for fish-farming, the frequency aberrant cells was about 3 to 7 times higher as compared to the control. An increased metal concentrations (1000-fold, 100-fold, and 2-fold for Cu, Zn and Ni, respectively) did not lead to a proportional increase in the frequency of aberrant cells which was only two times as much as that in control. Changes in the mitotic index were also disproportionate to the metal concentration in the solution. When combined, the metal ions had lower genotoxic effect as compared to their individual activity. The differences between separate and combined effects are indicative of ion competition. The calculated coefficients of antagonism in the experiment ranged from 0.20 to 0.40.

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“…Thus, MI changes were not proportional to increase in metal content in the solution. Similar phenomena were observes for other metals, for example, Cd [32,33].…”

Section: Introductionsupporting

confidence: 84%

CYTOGENETIC EFFECTS IN Allium сepa L. RESULTED FROM SEPARATE AND COMBINED EXPOSURE TO Cu, Zn and Ni

Ulyanenko¹,

Reva²,

Synzynys³

2017

S-h. biol.

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“…Indeed, in both studies carried out in different radioecological conditions, the high levels of genotoxicity are observed in all samples tested, with the important contribution from severe types of cell damage. The present work and our previous studies [7][8][9] show that an adequate environment quality assessment cannot rely only on information about pollutants concentrations. Authorities in charge of controlling the levels of industrial load have dealt with limited lists of toxicants.…”

Section: Discussionsupporting

confidence: 52%

“…Authorities in charge of controlling the levels of industrial load have dealt with limited lists of toxicants. Furthermore, a mutual intensification of the effects from environmental contaminants is very possible, especially when they occur in situations of low-level exposures, as demonstrated earlier [8,9]. It is therefore impossible to estimate the biological risk from a combined exposure if based only on the knowledge of contaminants levels in the environment.…”

Section: Discussionmentioning

confidence: 99%

Bioindication-based estimates as an integral part of the environment quality assessment

et al. 2009

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Abstract.A complex approach for the environment health assessment integrating information on contaminants levels registered in environment compartments and bioindication-based estimates is developed and applied to two studies carried out at sites with different scenarios of long-term impact. The environment state is assessed at the territory of a nuclear waste storage facility, Obninsk, Russia, and in the post-mining areas of the Upper Silesia, Poland with enhanced levels of natural radioactivity. Concentrations of micro-, macroelements and appropriate radionuclides activities are measured in samples of water and sediment. With the Allium cepa plant system, phyto-and genotoxicity of the sampled water and soil is estimated to assess a hazardous potential of the complex contamination. As biological endpoints, frequency and spectrum of chromosome aberrations and mitotic abnormalities in ana-telophase cells as well as mitotic activity in Allium cepa root tips are used. An approach and techniques are developed to reveal factors and contamination components that play a governing role in an induction of the observed biological effect and estimate their relative contribution. Compatibility of findings observed and legislative standards adopted are discussed. From the studies carried out, the Allium-test proved to be a sensitive and simple bioassay efficient in genotoxic monitoring of environmental pollutants.

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“…The ability of compound Zn-1 to change erythrocyte sensitivity to Triton X-100 was estimated by the interaction coefficient (Kw) calculated using the equation (b). [23] ( ) ( ) ( )…”

Section: Cytotoxicity Of New Metal Porphyrinates Based On Chlorophyllmentioning

confidence: 99%

“…After the treatment with Zn-1 for 1 h, Triton X-100 was added into the control and experimental erythrocyte suspensions and then the cell death dynamics was assessed (Figure 7). At the same time the hemolysis level was estimated in the samples not treated with Triton X-100, which allowed to calculate [23] the interaction coefficient (Kw) of the two factors (Table 3). Analysis of the data presented indicates the synergistic action of Triton-Х-100 and Zn-1 (Kw values are significantly greater than 1).…”

Section: Expression Of Stress Responsive Genes Under Zn-1mentioning

confidence: 99%

Cytotoxicity of New Metal Porphyrinates Based on Chlorophyll a Derivatives

Pylina¹,

Shadrin²,

Шевченко³

et al. 2017

MHC

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Cytogenetic effects of combined radioactive (137Cs) and chemical (Cd, Pb, and 2,4-D herbicide) contamination on spring barley intercalar meristem cells

Cited by 31 publications

References 28 publications

CYTOGENETIC EFFECTS IN Allium сepa L. RESULTED FROM SEPARATE AND COMBINED EXPOSURE TO Cu, Zn and Ni

CYTOGENETIC EFFECTS IN Allium сepa L. RESULTED FROM SEPARATE AND COMBINED EXPOSURE TO Cu, Zn and Ni

Bioindication-based estimates as an integral part of the environment quality assessment

Cytotoxicity of New Metal Porphyrinates Based on Chlorophyll a Derivatives

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