Cytogenetic Effects of Carbendazim on Mouse Bone Marrow Cells

Ilyushina, Nataliya A.

doi:10.1134/s1022795420090094

Cited by 6 publications

(3 citation statements)

References 40 publications

(39 reference statements)

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“…Установлено, что карбендазим вызывает анеугенные эффекты [10][11]. В ряде исследований in vivo найдена взаимосвязь между действием карбендазима и развитием гепатоцеллюлярных опухолей [12].…”

Section: Discussionunclassified

The study of primary DNA damage in the bone marrow of mice under the combined action of pesticides

et al. 2021

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Introduction. The study of the potential negative effects of combinations of several pesticide active ingredients is an important and understudied area of toxicological and hygienic research. The initial phase of the genotoxicant action on the genetic structures in cells is the primary DNA damage, the identification of which makes it possible to assess the early stages of the genotoxic effect of xenobiotics and their mixtures. The DNA comet assay is widely used for these purposes. The aim of the research is to assess the primary DNA damage under the combined action of pesticides. Materials and methods. To assess DNA damage the experiments on CD-1 mice of both sexes were performed using alkaline comet analysis. The concentration of active products reacting with thiobarbituric acid (TBA) in the blood serum of white outbred rats was assessed as a marker of lipid peroxidation. Results. It was found that mixtures of 2,4-D-acid + glyphosate and thiram + carbendazim did not cause the formation of breaks and alkali-labile sites in the DNA of mice bone marrow cells. Exposure to the combination of the technical grade active ingredients captan and fludioxonil induced the breaks and alkali-labile sites in the DNA of animal bone marrow cells. The comparison of the genotoxicity assessment results obtained by the comet assay and results of analysis of the TBA-active product concentrations in the rat blood serum suggests that the observed primary DNA damage upon exposure to the captan and fludioxonil combination can be mediated by the induction of lipid peroxidation and subsequent interaction of the resulting products with nucleic acids. Conclusion. The results indicate that some pesticides in combination can damage hereditary material in mammalian cells. Therefore, in order to ensure the safe use of pesticides for public health it is necessary to take into account the data on the genotoxicity not only of individual pesticide technical grade active ingredients but also their combinations.

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Section: Discussionunclassified

The study of primary DNA damage in the bone marrow of mice under the combined action of pesticides

et al. 2021

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“…Group II: carbendazim intoxication group (24 rats). Carbendazim groups were treated with orally a daily dose (250 mg/kg of B.W) of carbendazim for 30 days according to (17). The positive control group, the second, the third and the fourth groups were received a daily oral dose of ethanol extract of kumquat (100 ,200, and 300 mg/kg of B.W) respectively.…”

Section: Experimental Designmentioning

confidence: 99%

التأثير الوقائي لمستخلص فاکهة الکمکوات الإيثانولي ضد تسمم الکبد الناتج عن الکاربيندازيم في الفئران

السدودي¹,

محمد²,

بدر³

2022

مجلة الاقتصاد المنزلي.جامعة المنوفية

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Carbendazim is extensively used to control various fungal pathogens. Carbendazim is a contaminant found in food, soil, and water, causing functional disorders and tissue changes. This study aimed to evaluate the impact of ethanolic kumquat fruit against carbendazim induced liver toxicity in rats. Thirty adult male albino rats were randomly divided into two main groups and fed on the standard diet. Group I: negative control (6 rats) and group II: carbendazim groups (24 rats). Carbendazim groups were treated with orally a daily dose of 250 mg/kg of body weight (B.W) carbendazim for 30 days after being divided into equal four subgroups (6 rats each), the first: positive control group, the second, the third, and the fourth groups gave a daily oral dose of ethanol extract of kumquat (100, 200 and 300 mg/kg) B.W, respectively. The rats were sacrificed at the end of the experiment (30 days). The serum was analyzed for liver enzymes, serum glucose, kidney functions, antioxidant tests and lipids profile. The results elucidated that the oral administration of carbendazim (250 mg/kg of B.W) significantly increased liver enzymes, kidney functions, and glucose and induced the antioxidant system suppression in the positive control group compared with the other groups. The oral administration with ethanol extract of kumquat reduced the level of liver enzymes, kidney functions, glucose, and the antioxidant system compared with the positive control group. The study concluded that the ethanol extract of the kumquat is more effective in reducing the toxic effect of carbendazim in experimental animals.

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“…They were the first broad-spectrum systemic fungicides, also showing post-infection action, used mainly for foliar diseases or for seed dressing, and were characterized by low use rates [ 32 , 33 ]. Even though initially they were classified as having low toxicity to mammals and human, later negative effects on their reproduction and development were demonstrated [ 34 , 35 ], and in consequence, they were withdrawn from the EU agriculture, with the last active ingredient, thiophanate-methyl, having expired on 19 October 2020 [ 36 ]. The mode of action of MBC fungicides was elucidated in 1973 [ 37 ]; they inhibit microtubule assembly in diverse fungal species by binding to β-tubulin subunit and interfering with the polymerization, with subsequent disruption of the nuclear division, polarized growth, germ tube elongation, and mycelium growth.…”

Section: Fungicides Used For Grapevine Powdery Mildew Management and Resistance In E Necatormentioning

confidence: 99%

Grapevine Powdery Mildew: Fungicides for Its Management and Advances in Molecular Detection of Markers Associated with Resistance

et al. 2021

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Grapevine powdery mildew is a principal fungal disease of grapevine worldwide. Even though it usually does not cause plant death directly, heavy infections can lead to extensive yield losses, and even low levels of the disease can negatively affect the quality of the wine. Therefore, intensive spraying programs are commonly applied to control the disease, which often leads to the emergence and spread of powdery mildew strains resistant to different fungicides. In this review, we describe major fungicide classes used for grapevine powdery mildew management and the most common single nucleotide mutations in target genes known to confer resistance to different classes of fungicides. We searched the current literature to review the development of novel molecular methods for quick detection and monitoring of resistance to commonly used single-site fungicides against Erysiphe necator. We analyze and compare the developed methods. From our investigation it became evident that this research topic has been strongly neglected and we hope that effective molecular methods will be developed also for resistance monitoring in biotroph pathogens.

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Cytogenetic Effects of Carbendazim on Mouse Bone Marrow Cells

Cited by 6 publications

References 40 publications

The study of primary DNA damage in the bone marrow of mice under the combined action of pesticides

The study of primary DNA damage in the bone marrow of mice under the combined action of pesticides

التأثير الوقائي لمستخلص فاکهة الکمکوات الإيثانولي ضد تسمم الکبد الناتج عن الکاربيندازيم في الفئران

Grapevine Powdery Mildew: Fungicides for Its Management and Advances in Molecular Detection of Markers Associated with Resistance

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