“…Generally, the percentage of abnormal PMCs in meiotic division II was higher than that observed in meiotic division I. A similar effect was observed by Amer et al (1969) in Vicia faba plants after chronic irradiation with X-rays and by Amer et al (1989) in Vicia faba after fumigation with SO2. Table 1 demonstrated the types and percentages of different meiotic abnormalities observed due to spraying Vicia faba plants with used concentrations of heavy metals .…”
Section: Cytological Analysissupporting
confidence: 80%
“…4 were observed at a considerable percentage in the different meiotic stages (Table 1). Similar results were obtained after using different chemicals, SO2 (Amer et al 1989), HF (Mohamed 1970), ozone (Rajeshwari and Harney 1977) and heavy metals (Bipasha and Shella 1992).…”
The three heavy metals Pb, Hg and Cd were examined for the induction of meiotic abnormalities and changes in the M2 seed storage protein banding patterns of Vicia faba plants. The used metal nitrate solutions induced a wide range of meiotic abnormalities. The most abundant of them were stickiness, break, lagging, bridges and disturbed phases. At the level of M2 seed storage protein, the 3 tested metals caused changes in the protein banding patterns especially at the high molecular weight regions. The results showed that lead and mercury have more mutagenic potentialities than cadmium. Rapid urbanization and industrialization have enhanced the levels of toxic heavy metals in the environment posing a potential health hazard for all living organisms (Lewis and Mc Intosh 1989, Di Domenico et al. 1989). Heavy metals have some genotoxic potential (Forni 1980, Gerber et al. 1980, IARC 1987, Winder 1989). All metals are probably toxic if ingested in sufficient amounts. The toxicity of a metal is greatly influenced by environmental factors as well as the presence of other metals (Moutschen 1985). The present investigation was planned to study the mutagenic potentialities of the heavy metals Pb, Hg and Cd as revealed by meiotic abnormalities and changes in M2 seed storage protein banding patterns of Vicia faba plants. Material and methods Cytological experiment Vicia faba plants were sprayed at the flowering stage with 5 concentrations of lead nitrate, mercric nitrate and cadmium nitrate solutions (10, 25, 50, 100, 200 ppm) twice for two successive days. Control plants were sprayed with distilled water. Three pots (6 plants/each) were used for each concentration. The treated plants were 40 days old at the time of gathering of the flower buds, which were gathered 24 h after treatment, fixed; immediately in Carnoy's fluid (3 : 1 ethyl alcohol : glacial acetitic acid) and examined using the aceto-carmine smear method (Belling 1926) under light microscope (Reicher model No. 1706 V). Abnormalities were counted in the first and second meta, ana and telophases. The number of the investigated pollen mother cells (PMCs)/treatment was 1389-2573. Biochemical genetic monitoring SDS-polyacrylamide gel electrophoresis procedure was carried out according to Laemmli (1970). The molecular weight of the resulted protein banding patterns were estimated according to Matta et al. (1981). The dry M2 seeds of Vicia faba plants (5 g) whose parents were previously sprayed with either heavy metals, were milled to fine powder. Total proteins from 0.2 g of milled seeds were extracted overnight using 0.2 M Tris-HCl buffer, pH 6.8 containing 2% SDS and 10% glycerol. Centrifugation was carried out at 9000 rpm for 6 min, then 30,u1 supernatant were loaded 76•@
“…Generally, the percentage of abnormal PMCs in meiotic division II was higher than that observed in meiotic division I. A similar effect was observed by Amer et al (1969) in Vicia faba plants after chronic irradiation with X-rays and by Amer et al (1989) in Vicia faba after fumigation with SO2. Table 1 demonstrated the types and percentages of different meiotic abnormalities observed due to spraying Vicia faba plants with used concentrations of heavy metals .…”
Section: Cytological Analysissupporting
confidence: 80%
“…4 were observed at a considerable percentage in the different meiotic stages (Table 1). Similar results were obtained after using different chemicals, SO2 (Amer et al 1989), HF (Mohamed 1970), ozone (Rajeshwari and Harney 1977) and heavy metals (Bipasha and Shella 1992).…”
The three heavy metals Pb, Hg and Cd were examined for the induction of meiotic abnormalities and changes in the M2 seed storage protein banding patterns of Vicia faba plants. The used metal nitrate solutions induced a wide range of meiotic abnormalities. The most abundant of them were stickiness, break, lagging, bridges and disturbed phases. At the level of M2 seed storage protein, the 3 tested metals caused changes in the protein banding patterns especially at the high molecular weight regions. The results showed that lead and mercury have more mutagenic potentialities than cadmium. Rapid urbanization and industrialization have enhanced the levels of toxic heavy metals in the environment posing a potential health hazard for all living organisms (Lewis and Mc Intosh 1989, Di Domenico et al. 1989). Heavy metals have some genotoxic potential (Forni 1980, Gerber et al. 1980, IARC 1987, Winder 1989). All metals are probably toxic if ingested in sufficient amounts. The toxicity of a metal is greatly influenced by environmental factors as well as the presence of other metals (Moutschen 1985). The present investigation was planned to study the mutagenic potentialities of the heavy metals Pb, Hg and Cd as revealed by meiotic abnormalities and changes in M2 seed storage protein banding patterns of Vicia faba plants. Material and methods Cytological experiment Vicia faba plants were sprayed at the flowering stage with 5 concentrations of lead nitrate, mercric nitrate and cadmium nitrate solutions (10, 25, 50, 100, 200 ppm) twice for two successive days. Control plants were sprayed with distilled water. Three pots (6 plants/each) were used for each concentration. The treated plants were 40 days old at the time of gathering of the flower buds, which were gathered 24 h after treatment, fixed; immediately in Carnoy's fluid (3 : 1 ethyl alcohol : glacial acetitic acid) and examined using the aceto-carmine smear method (Belling 1926) under light microscope (Reicher model No. 1706 V). Abnormalities were counted in the first and second meta, ana and telophases. The number of the investigated pollen mother cells (PMCs)/treatment was 1389-2573. Biochemical genetic monitoring SDS-polyacrylamide gel electrophoresis procedure was carried out according to Laemmli (1970). The molecular weight of the resulted protein banding patterns were estimated according to Matta et al. (1981). The dry M2 seeds of Vicia faba plants (5 g) whose parents were previously sprayed with either heavy metals, were milled to fine powder. Total proteins from 0.2 g of milled seeds were extracted overnight using 0.2 M Tris-HCl buffer, pH 6.8 containing 2% SDS and 10% glycerol. Centrifugation was carried out at 9000 rpm for 6 min, then 30,u1 supernatant were loaded 76•@
“…Faba bean (Vicia faba V. Giza 2) plants were exposed to SO2 concentrations of 13,095 to 654,750 |Lig m"^for 5 h or 13,095 jag m'^for 5 h d"^for 1, 2 or 4 days to determine if there were cytogenetic or viability effects on pollen (Amer et al, 1989). The number of non-viable pollen grains increased significantly as the concentration of SO2 increased.…”
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