Cytogenetic description of Ancistrus abilhoai (Siluriformes: Loricariidae) from Iguaçu River basin, southern Brazil

Ribeiro, Marcos Otávio; Noleto, Rafael Bueno; Lorscheider, Carla Andreia; Porto, Fernanda Errero; Prizon, Ana Camila; Zawadzki, Cláudio Henrique; Oliveira, Luciene Castuera de; Portela-Castro, Ana Luiza de Brito

doi:10.4238/2015.april.27.20

Cited by 9 publications

(3 citation statements)

References 26 publications

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“…For the same characteristics, except for 2n, we can observe in P. cavatica and P. vittata the presence of a 5S rDNA site in the interstitial region in the same chromosome with the single NOR, which may be a primitive remnant condition for the Peckoltia clade, as found in Hypancistrus cf. debilittera 12 and Panaqolus sp., 13 belonging to the same clade, and in the Ancistrus 6,9,35,40 genus that is of the same subfamily Hypostominae. 5 Data relating to organization of rDNA clusters indicate that other types of chromosome rearrangements in addition to inversions should have occurred during karyotype divergence of these species.…”

Section: Discussionmentioning

confidence: 99%

In Situ Localization of Ribosomal Sites in Peckoltia and Ancistomus (Loricariidae: Hypostominae) from the Amazon Basin

et al. 2018

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Loricariidae is a diverse group of fish from the neotropical region, occupying a wide variety of freshwater environments. Cytogenetic data have brought important insights into Loricariidae diversity because they help validate undescribed species as well as our understanding of inter- and intraspecific diversity. However, conventional cytogenetic approaches are limited in their ability to detect variability in some lineages, as seen in the Peckoltia clade, owing to their apparent conserved karyotype. Thus, the aim of this work was to map 5S and 18S ribosomal (rDNA) sites in five species of Peckoltia and one species of Ancistomus from the Amazon basin, and discusses the mechanisms of organization and diversification of these clusters. The species analyzed were found to have 2n = 52 and share KF = 38 m-sm +14st-a chromosomes, except Peckoltia vittata with KF = 34 m-sm +18st-a. Extensive variations in the number and location of 5S and 18S rDNA sites were observed among species. These data indicate that inversions are not the most important events in karyotype evolution in this group, and should prove useful in identifying the species studied here. In addition to inversions, transpositions are important evolutionary events that are involved at least in rDNA clusters spreading in Peckoltia and probably in other species of Hypostominae.

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Section: Discussionmentioning

confidence: 99%

In Situ Localization of Ribosomal Sites in Peckoltia and Ancistomus (Loricariidae: Hypostominae) from the Amazon Basin

et al. 2018

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“…The rRNA in eukaryotes presents as two subunits (one formed by 28S, 18S and 5.8S and another one formed by 5S) and their DNA sequences vary very slowly due to selective pressure, being considered highly conserved (Long and Dawid 1980). This allows the interspecific hybridization of the mentioned probes (obtained from Prochilodus argenteus and Megaleporinus elongatus ), with chromosomes of a wide variety of fishes, like Characiformes (de Marco Ferro et al 2001, Pazza et al 2006, da Silva et al 2016), Gymnotiformes (Fernandes et al 2017a, 2017b) Perciformes (Jacobina et al 2014, Argôlo and Affonso 2015, Oliveira et al 2015b), Siluriformes (Blanco et al 2014, Kantek et al 2015, Ribeiro et al 2015), including other species of Hypostomus (Kavalco et al 2004a, 2005, Traldi et al 2013, Baumgärtner et al 2014, Oliveira et al 2015a, Lara Kamei et al 2017). …”

Section: Discussionmentioning

confidence: 99%

Studies in two allopatric populations of Hypostomus affinis (Steindachner, 1877): the role of mapping the ribosomal genes to understand the chromosome evolution of the group

Brandão¹,

Reis²,

Garcia³

et al. 2018

CCG

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Several cytogenetic markers show chromosomal diversity in the fish such as “armoured catfish”. Although studies have characterized many species in the major genera representing these Siluridae, particularly in the genus Hypostomus Lacépède, 1803, trends in chromosome evolution of this group remain unclear. The Paraíba do Sul river basin contains the armoured catfish Hypostomus affinis Steindachner, 1877, which is unique because of its distribution of repetitive DNAs, the 5S and 18S rDNA. Identified samples and registered collections in Brazilian museums were identified as the same typological species, while we observed wide variations in the physical location of this gene in the karyotype based on fluorescent in situ hybridization results. In this study, we propose that these species can represent evolutionarily independent units, as these fish frequently undergo processes such as dispersion and vicariance and that the rDNA is associated with DNA that spreads in the genome, such as transposons. Additionally, the absence of gene flow due to the distance of the sample location could intensify evolutionary processes. The phenotypes found for the 18S rDNA showed minor changes in relation to the number of sites between the lower and upper drainage regions of Paraíba do Sul. The large difference in the number of sites found for the 5S rDNA entered the same region (upper drainage of the basin) and the literature data could represent a population dynamics where an expansion of the 5S rDNA sites provides an extinct or non-sampled cytotype in this work.

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“…Other plesiomorphic conditions for Loricariidae include the 18S and 5S rDNA sequences in a single pair of meta/ submetacentric chromosomes (Ziemniczak et al, 2012). However, in the Ancistrini tribe, both the synteny and nonsynteny between the 18S and 5S sequences are commonly observed (Mariotto et al, 2011;Ribeiro et al, 2015;Favarato et al, 2016;Prizon et al, 2016;Pety et al, 2018), showing the huge chromosome sites variation in the karyotypes of this group (Pansonato-Alves et al, 2013;Bueno et al, 2014;Pety et al, 2018). The mapping of 18S and the 5S sequences in the karyotypes here described is in agreement with that observed in other species of Peckoltia; in which extensive dispersion of these genes is observed (Pety et al, 2018) (Table 1).…”

Section: Karyotype Diversity In Peckoltiamentioning

confidence: 99%

Comparative Cytogenetics Analysis Among Peckoltia Species (Siluriformes, Loricariidae): Insights on Karyotype Evolution and Biogeography in the Amazon Region

Silva

Souza²,

Pety

et al. 2021

Front. Genet.

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Peckoltia is widely distributed genus in the Amazon and Orinoco basins and the Guiana Shield, containing 18 valid species, and distinct morphotypes still needing description in the scientific literature due to its great taxonomic complexity. This study performed a comparative chromosomal analysis of two undescribed Peckoltia species (Peckoltia sp. 3 Jarumã and Peckoltia sp. 4 Caripetuba) from the Brazilian Amazon using conventional chromosome bands methods and in situ localization of the repetitive DNA (5S and 18S rRNA and U1 snRNA genes and telomeric sequences). Both species presented 2n = 52 but differed in their karyotype formula, probably due to inversions or translocations. The nucleolus organizer regions (NORs) showed distal location on a probably homeologous submetacentric pair in both species, besides an extra signal in a subtelocentric chromosome in Peckoltia sp. 4 Caripetuba. Heterochromatin occurred in large blocks, with different distributions in the species. The mapping of the 18S and 5S rDNA, and U1 snDNA showed differences in locations and number of sites. No interstitial telomeric sites were detected using the (TTAGGG)n probes. Despite 2n conservationism in Peckoltia species, the results showed variation in karyotype formulas, chromosomal bands, and locations of repetitive sites, demonstrating great chromosomal diversity. A proposal for Peckoltia karyotype evolution was inferred in this study based on the diversity of location and number of chromosomal markers analyzed. A comparative analysis with other Peckoltia karyotypes described in the literature, their biogeography patterns, and molecular phylogeny led to the hypothesis that the derived karyotype was raised in the left bank of the Amazon River.

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Cytogenetic description of Ancistrus abilhoai (Siluriformes: Loricariidae) from Iguaçu River basin, southern Brazil

Cited by 9 publications

References 26 publications

In Situ Localization of Ribosomal Sites in Peckoltia and Ancistomus (Loricariidae: Hypostominae) from the Amazon Basin

In Situ Localization of Ribosomal Sites in Peckoltia and Ancistomus (Loricariidae: Hypostominae) from the Amazon Basin

Studies in two allopatric populations of Hypostomus affinis (Steindachner, 1877): the role of mapping the ribosomal genes to understand the chromosome evolution of the group

Comparative Cytogenetics Analysis Among Peckoltia Species (Siluriformes, Loricariidae): Insights on Karyotype Evolution and Biogeography in the Amazon Region

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