Cytogenetic Damage in Turkish Coke Oven Workers Exposed to Polycyclic Aromatic Hydrocarbons: Association with CYP1A1, CYP1B1, EPHX1, GSTM1, GSTT1, and GSTP1 Gene Polymorphisms

Ada, Ahmet Oğuz; Demiroglu, Canan; Yilmazer, Meltem; Süzen, Sinan; Demirbağ, Ali Eba; Efe, Sibel; Alemdar, Y.; İşcan, Mümtaz; Burgaz, Sema

doi:10.2478/10004-1254-64-2013-2328

Cited by 10 publications

(3 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chromatid breaks were the most common type of chromosomal aberrations reported in their study. A similar study by Ada et al (21) aimed to determine cytogenetic damage in peripheral blood lymphocytes in Turkish coke oven workers and showed that the overall frequencies of aberrant cells without gaps were significantly higher (p < 0.001) in coke oven workers than in the control group. Chromosomal aberrations mainly consisted of breaks and chromatid gaps.…”

Section: Discussionmentioning

confidence: 92%

Cytogenetic analysis of coke oven workers in Eastern Slovakia

Sedlák,

Bujňák,

Gajdoš

et al. 2023

Cent Eur J Public Health

View full text Add to dashboard Cite

Objective: Our study aimed to evaluate the extent of polycyclic aromatic hydrocarbon (PAH) exposure in coke oven workers from Eastern Slovakia by cytogenetic analysis of human peripheral lymphocytes.Methods: A total of 81 peripheral blood samples were collected from PAH-exposed workers (mean age 45.84 ± 9.73 years) and 30 samples constituted the control group (41.93 ± 15.39 years). The samples were processed using routine cytological analysis. Conventional cytogenetic analysis of human peripheral lymphocytes has been used to evaluate the effects of PAHs.Results: Comparison of the aberrant cells in the total exposed with the controls showed a significant difference (p < 0.05). A high level of significance (p < 0.001) was observed when comparing the gaps between the exposed group and the control group. There was a significant difference (p < 0.01) in aberrant cells and chromatid breaks (p < 0.05) in the GR1 working subgroup compared with the control group. The results of the correlation analysis did not show a significant relationship between the length of occupational exposure and the frequency of aberrant cells (r = 0.071, p = 0.529). Similarly, no association was observed between smoking among coke plant workers and the frequency of aberrant cells (r = 0.117, p = 0.538).Conclusion: Cytogenetic analysis showed an increased frequency of chromosomal aberrations in coke oven workers in Eastern Slovakia.

show abstract

Section: Discussionmentioning

confidence: 92%

Cytogenetic analysis of coke oven workers in Eastern Slovakia

Sedlák,

Bujňák,

Gajdoš

et al. 2023

Cent Eur J Public Health

View full text Add to dashboard Cite

show abstract

“…No study looks at both polymorphisms in the Turkish population. Ada et al [10], while looking at only CYP1B1*4 in people working in a coke oven, Bilgin et al [18] looked at NSCLC (Non-Small Cell Lung Cancer) patients and CYP1B1*4.…”

Section: Discussionmentioning

confidence: 99%

“…There are many studies regarding the relationship of these polymorphisms with glaucoma, obesity, cardiovascular diseases, and hormone-mediated cancers [8,9]. In previous studies on the Turkish population, Ada et al [10] investigated the CYP1B1*4 polymorphism in coke oven workers with 49 people. In their study, Güler et al [11] examined CYP1B1*2 and CYP1B1*3 polymorphisms in lung cancer patients.…”

Section: Introductionmentioning

confidence: 99%

Investigation of CYP1B13 and CYP1B14 polymorphisms in a Turkish population

et al. 2022

View full text Add to dashboard Cite

CYP1B1 is a P450 enzyme involved in activating pro-carcinogens to carcinogens as well as estrogen metabolism. In order to examine the effect of CYP1B1 on cancer metabolism, it should be compared with healthy individuals and whether the polymorphism between healthy individuals and sick individuals is significant. This study aims to screen the CYP1B1*3 and CYP1B1*4 polymorphisms of a group of individuals who have not been diagnosed with cancer to examine the genetic differences of metabolic enzymes in the Turkish population.This study is a cross-sectional type descriptive study.The study included 295 patients without a cancer diagnosis. The research sample includes patients who applied to Ankara University Medical Faculty Hospital and Afyonkarahisar Health Sciences University Research and Application Hospital. The individuals signed voluntary consent forms before participation, and 3 ml blood samples were taken from each. DNA samples were obtained using a DNA isolation kit, and then polymorphism was determined by real-time PCR. The distribution of CYP1B1*3 and CYP1B1*4 polymorphism in healthy individuals was determined. The frequency of CYP1B1*1/*1 (wild type), CYP1B1*1/*3 (heterozygous) and, CYP1B1*3/*3 (mutant) genotypes were found 39.33%, 50.67% and 10.0% respectively. The frequency of CYP1B1*1/*1 (wild type), CYP1B1*1/*4 (heterozygous) and, CYP1B1*4/*4 (mutant) genotypes were found 39.31%, 60.69% and 0% respectively. No individuals with mutant genotype were detected in this genotype (CYP1B1*4).The results show that the genotype frequencies of the CYP1B1*3 gene polymorphism in a Turkish population are similar to other Caucasian populations. However, it was determined that the Turkish population did not show similarity with other races in terms of CYP1B1*4 polymorphism.

show abstract