Cytogenetic characterization of the bay scallop, Argopecten irradians irradians, by multiple staining techniques and fluorescence in situ hybridization

Huang, Xiaoting; Hu, Jiang; Hu, Xiaoli; Zhang, Can; Zhang, Lingling; Wang, Shi; Lü, Wei; Bao, Zhenmin

doi:10.1266/ggs.82.257

Cited by 22 publications

(18 citation statements)

References 24 publications

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“…Single 5S rDNA cluster in the present V. aurea and T. rhomboides is concordant with the results obtained in the other two species of Veneridae (Ruditapes decussatus and R. philippinarum, Hurtado et al 2011), but differs from Cerastoderma edule (Cardiidae) showing five clusters of 5S rDNA on five different chromosome pairs (Insua et al 1999). Most species of Pectinidae also show a single cluster of 5S rDNA López-Piñón et al 2005;Insua et al 2006;Huang et al 2007b), but Aequipecten opercularis shows two clusters. For Argopecten irradians one (Huang et al 2007a) and two clusters have been described.…”

Section: Discussionsupporting

confidence: 91%

“…The situation in families of the other subclass of Bivalvia (Pteromorphia) is somewhat more variable. Major rDNAs occur in only one chromosome pair in most Ostreidae species analyzed (Cross et al 2003; and in half of the species of Pectinidae Insua et al 2006;Odierna et al 2006), but not in Hinnites distortus (Lopez-Piñon et al 2005), Patinopecten yessoensis (Huang et al 2007b), Argopecten irradians Huang et al 2007a), and A. purpuratus (Gajardo et al 2002). However, in Mytilidae, major rDNA was found in one (Brachidontes pharaonis, Vitturi et al 2000;B.…”

Section: Discussionmentioning

confidence: 99%

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Cytogenetic characterization and mapping of rDNAs, core histone genes and telomeric sequences in Venerupis aurea and Tapes rhomboides (Bivalvia: Veneridae)

et al. 2011

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We describe the chromosomal location of GC-rich regions, 28S and 5S rDNA, core histone genes, and telomeric sequences in the veneroid bivalve species Venerupis aurea and Tapes (Venerupis) rhomboides, using fluorochrome staining with propidium iodide, DAPI and chromomycin A3 (CMA) and fluorescent in situ hybridization (FISH). DAPI dull/CMA bright bands were coincident with the chromosomal location of 28S rDNA in both species. The major rDNA was interstitially clustered at a single locus on the short arms of the metacentric chromosome pair 5 in V. aurea, whereas in T. rhomboides it was subtelomerically clustered on the long arms of the subtelocentric chromosome pair 17. 5S rDNA also was a single subtelomeric cluster on the long arms of subtelocentric pair 17 in V. aurea and on the short arms of the metacentric pair 9 in T. rhomboides. Furthermore, V. aurea showed four telomeric histone gene clusters on three metacentric pairs, at both ends of chromosome 2 and on the long arms of chromosomes 3 and 8, whereas histone genes in T. rhomboides clustered interstitially on the long arms of the metacentric pair 5 and proximally on the long arms of the subtelocentric pair 12. Double and triple FISH experiments demonstrated that rDNA and H3 histone genes localized on different chromosome pairs in the two clam species. Telomeric signals were found at both ends of every single chromosome in both species. Chromosomal location of these three gene families in two species of Veneridae provides a clue to karyotype evolution in this commercially important bivalve family.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Cytogenetic characterization and mapping of rDNAs, core histone genes and telomeric sequences in Venerupis aurea and Tapes rhomboides (Bivalvia: Veneridae)

et al. 2011

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“…The presence of a single site for 5S rDNA observed in the 4 Proscopiidae species analyzed here is relatively common in eukaryotes and has been reported in vertebrates and some mollusks [Mandrioli, 2000;Sola et al, 2000;Martins and Galetti, 2001;López-Piñón et al, 2005;Insua et al, 2006;Huang et al, 2007]. In grasshoppers, the 5S rDNA sequences have been previously mapped in only 2 species, Eyprepocnemis plorans from 3 populations [Cabrero et al, 2003] and Rammathocerus brasiliensis [Loreto et al, 2008].…”

Section: Discussionsupporting

confidence: 57%

Cytogenetic Mapping of 5S and 18S rRNAs and H3 Histone Genes in 4 Ancient Proscopiidae Grasshopper Species: Contribution to Understanding the Evolutionary Dynamics of Multigene Families

Cabral-de-Mello

Martins

Souza

et al. 2010

Cytogenet Genome Res

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This paper reports on the chromosomal location of 18S rRNA, 5S rRNA and H3 histone multigene families in 4 species of a relatively ancient and diversified group of grasshoppers belonging to the family Proscopiidae. The 5S rRNA and H3 histone genes were highly conserved in the number of sites and chromosomal position in the 4th chromosome pair in all species analyzed, whereas the 18S rRNA genes showed slightly more variation because they were present on one or 2 chromosome pairs, depending on the species. The 5S and 18S rRNA gene families occurred in different chromosomes; in contrast, H3 histone and 5S rRNA genes co-localized in the same chromosomal position, with an apparently interspersed organization. Considering that the Proscopiidae family is a relatively ancient group compared with the Acrididae family, the association of the H3 histone and 5S rRNA multigene families can represent a basal condition for grasshoppers, although more research is needed on other representatives of this insect group to confirm this statement. The presence of such an association of 5S rDNA and H3 histone in mussels and arthropods (beetles, grasshoppers and crustaceans) suggests that this linked configuration could represent an ancestral pattern for invertebrates. These results provide new insights into the understanding of the genome organization and the evolution of multigene families in grasshoppers and in insects as a whole.

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“…The presence of only one 5S rDNA site is common among eukaryotes, and the distinct chromosomal locations of 5S and 18S rDNA sites has been frequently reported for vertebrates (Mandrioli et al, 2000;Sola et al, 2000;Martins and Galetti, 2001). In protostome invertebrates, this kind of arrangement was described in some mollusks (López-Piñ ón et al, 2005;Insua et al, 2006;Huang et al, 2007). On the other hand, some studies have shown a different scenario for rDNA location, with colocalization of these sequences in protostomes, as was reported for the Annelida, Octodrilus complanatus , three mollusk species Vitturi et al, 2004;Wang and Guo, 2004) and in seven calanoid copepods-Crustacea (Drouin and Moniz de Sá, 1995), which presented an association of repeated 5S and 18S DNA sequences, as shown by Southern blotting.…”

Section: Mapping Of Repetitive Dnas In D Geminatus Dc Cabral-de-mellmentioning

confidence: 86%

Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

2009

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Chromosomal banding techniques and repetitive DNA mapping are useful tools in comparative analysis and in the elucidation of genome organization of several groups of eukaryotes. In this study, we contributed to the knowledge of Coleoptera genomes by reporting the chromosomal organization of repetitive DNA sequences, as well as the presence and characteristics of a B chromosome in two natural populations of Dichotomius geminatus (Coleoptera; Scarabaeidae) using classical, chromosomal banding and molecular cytogenetic techniques. As in other coleopteran species, the heterochromatin was mainly concentrated in pericentromeric regions and the B chromosome was composed almost entirely of heterochromatin. Physical mapping using double fluorescent in situ hybridization was performed for the first time in Coleoptera; using DNA probes for 5S and 18S ribosomal RNA (rRNA) and histone H3 genes, we showed that ribosomal 18S rDNAs are located in chromosomes 3 and 4, whereas 5S rRNA and histone H3 genes are colocalized in chromosomal pair 2 and show an apparently interspersed organization. Moreover, these genes are not present in the B chromosome, suggesting that the B chromosome did not originate from chromosomal pairs 2, 3 or 4. On the other hand, mapping of the C 0 t-1 DNA fraction showed that the B chromosome is enriched in repetitive DNA elements, also present in the standard complement, indicating an intraspecific origin of this element in D. geminatus. These results will contribute to our understanding of genome organization and evolution of repetitive elements in Coleoptera and other insects regarding both A and B chromosomes.

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Cytogenetic characterization of the bay scallop, Argopecten irradians irradians, by multiple staining techniques and fluorescence in situ hybridization

Cited by 22 publications

References 24 publications

Cytogenetic characterization and mapping of rDNAs, core histone genes and telomeric sequences in Venerupis aurea and Tapes rhomboides (Bivalvia: Veneridae)

Cytogenetic characterization and mapping of rDNAs, core histone genes and telomeric sequences in Venerupis aurea and Tapes rhomboides (Bivalvia: Veneridae)

Cytogenetic Mapping of 5S and 18S rRNAs and H3 Histone Genes in 4 Ancient Proscopiidae Grasshopper Species: Contribution to Understanding the Evolutionary Dynamics of Multigene Families

Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

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