Cytogenetic bio-dosimetry techniques in the detection of dicentric chromosomes induced by ionizing radiation: A review

Ludovici, Gian Marco; Cascone, Maria Grazia; Huber, Thomas S.; Chierici, Andrea; Gaudio, P.; Souza, S.O.; D’Errico, Francesco; Malizia, A.

doi:10.1140/epjp/s13360-021-01447-3

Cited by 16 publications

(13 citation statements)

References 135 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For further validation, quantitative in vitro studies could be conducted to study the radiation response of lymphocytes with different treatment modalities and parameters (Heylmann et al 2021). In addition, cytogenetic bio-dosimetry methods such as counting the frequency of dicentric chromosomes in peripheral blood lymphocytes could be considered, where the frequency of dicentric chromosomes increases with radiation dose (Kelly and Brown 1965, Ludovici et al 2021. With development of detailed vasculature also in animal models (Fung et al 2011, Peeters et al 2017, validation with in vitro and in vivo experiments would be of great interest to evaluate the accuracy of dose estimation and the clinical translation feasibly.…”

Section: Discussionmentioning

confidence: 99%

A dynamic blood flow model to compute absorbed dose to circulating blood and lymphocytes in liver external beam radiotherapy

et al. 2022

View full text Add to dashboard Cite

We have developed a novel 4D dynamic liver blood flow model, capable of accurate dose estimation to circulating blood cells during liver-directed external beam radiotherapy, accounting for blood recirculation and radiation delivery time structure. Adult male and adult female liver computational phantoms with detailed vascular trees were developed to include the hepatic arterial, hepatic portal venous, and hepatic venous trees. A discrete time Markov Chain approach was applied to determine the spatiotemporal distribution of 105 blood particles (BP) in the human body based on reference values for cardiac output and organ blood volumes. For BPs entering the liver, an explicit Monte Carlo simulation was implemented to track their propagation along ~2000 distinct vascular pathways through the liver. The model tracks accumulated absorbed dose from time-dependent radiation fields with a 0.1s time resolution. The computational model was then evaluated for 3 male and 3 female patients receiving photon (VMAT and IMRT) and proton (passive SOBP and active PBS) treatments. The dosimetric impact of treatment modality, delivery time, and fractionation on circulating blood cells was investigated and quantified using the mean dose (μdose,b), V>0Gy, V>0.125Gy, and D2%. Average reductions in μdose,b, V>0Gy, V>0.125Gy, and D2% of 45%, 6%, 53%, 19% respectively, were observed for proton treatments as compared to photon treatments. Our simulation also showed that V>0Gy, V>0.125Gy, and D2% were highly sensitive to the beam-on time. Both V>0Gy and V>0.125Gy increased with beam-on time, whereas D2% decreased with increasing beam-on time, demonstrating the tradeoff between low dose to a large fraction of blood cells and high dose to a small fraction of blood cells. Consequently, proton treatments are not necessarily advantageous in terms of dose to the blood simply based on integral dose considerations. Instead, both integral dose and beam-on time can substantially impact relevant dosimetric indices.

show abstract

Section: Discussionmentioning

confidence: 99%

A dynamic blood flow model to compute absorbed dose to circulating blood and lymphocytes in liver external beam radiotherapy

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The dose response for induction of point mutations is linear, while that for deletions fits a linear quadratic response [ 64 , 65 ]. Chromosomal aberrations as dicentric chromosomes have a linear quadratic dose response [ 66 ]. In case of chromosomal translocations, the data are not consistent and suggest a linear [ 67 , 68 ] or linear quadratic dose response [ 69 , 70 ].…”

Section: Ir Induced Leukemiamentioning

confidence: 99%

Preleukemic Fusion Genes Induced via Ionizing Radiation

Košík

Skorvaga

Belyaev

2023

IJMS

View full text Add to dashboard Cite

Although the prevalence of leukemia is increasing, the agents responsible for this increase are not definitely known. While ionizing radiation (IR) was classified as a group one carcinogen by the IARC, the IR-induced cancers, including leukemia, are indistinguishable from those that are caused by other factors, so the risk estimation relies on epidemiological data. Several epidemiological studies on atomic bomb survivors and persons undergoing IR exposure during medical investigations or radiotherapy showed an association between radiation and leukemia. IR is also known to induce chromosomal translocations. Specific chromosomal translocations resulting in preleukemic fusion genes (PFGs) are generally accepted to be the first hit in the onset of many leukemias. Several studies indicated that incidence of PFGs in healthy newborns is up to 100-times higher than childhood leukemia with the same chromosomal aberrations. Because of this fact, it has been suggested that PFGs are not able to induce leukemia alone, but secondary mutations are necessary. PFGs also have to occur in specific cell populations of hematopoetic stem cells with higher leukemogenic potential. In this review, we describe the connection between IR, PFGs, and cancer, focusing on recurrent PFGs where an association with IR has been established.

show abstract

“…Ionizing radiation can deposit energy in a system and it is ubiquitous in the environment. Its source can be natural such as radioactive materials and cosmic rays or artificial, such as nuclear power plants [10]. Recently, there has been a strong interest in the health of organisms in radioactively contaminated sites such as those of Chernobyl and Fukushima Dai-ichi.…”

Section: The Effects Of Ionizing Radiation On Plantsmentioning

confidence: 99%

Effects of Ionizing Radiation on Flora Ten Years after the Fukushima Dai-ichi Disaster

Ludovici

Chierici

Souza

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

The aim of this work is to analyze the effects of ionizing radiation and radionuclides (like 137Cs) in several higher plants located around the Fukushima Dai-ichi Nuclear Power Plant (FNPP), evaluating both their adaptive processes and evolution. After the FNPP accident in March 2011 much attention was focused to the biological consequences of ionizing radiation and radionuclides released in the area surrounding the nuclear plant. This unexpected mishap led to the emission of radionuclides in aerosol and gaseous forms from the power plant, which contaminated a large area, including wild forest, cities, farmlands, mountains, and the sea, causing serious problems. Large quantities of 131I, 137Cs, and 134Cs were detected in the fallout. People were evacuated but the flora continued to be affected by the radiation exposure and by the radioactive dusts’ fallout. The response of biota to FNPP irradiation was a complex interaction among radiation dose, dose rate, temporal and spatial variation, varying radiation sensitivities of the different plants’ species, and indirect effects from other events. The repeated ionizing radiations, acute or chronic, guarantee an adaptation of the plant species, demonstrating a radio-resistance. Consequently, ionizing radiation affects the genetic structure, especially during chronic irradiation, reducing genetic variability. This reduction is associated with the different susceptibility of plant species to chronic stress. This would confirm the adaptive theory associated with this phenomenon. The effects that ionizing radiation has on different life forms are examined in this review using the FNPP disaster as a case study focusing the attention ten years after the accident.

show abstract

Cytogenetic bio-dosimetry techniques in the detection of dicentric chromosomes induced by ionizing radiation: A review

Cited by 16 publications

References 135 publications

A dynamic blood flow model to compute absorbed dose to circulating blood and lymphocytes in liver external beam radiotherapy

A dynamic blood flow model to compute absorbed dose to circulating blood and lymphocytes in liver external beam radiotherapy

Preleukemic Fusion Genes Induced via Ionizing Radiation

Effects of Ionizing Radiation on Flora Ten Years after the Fukushima Dai-ichi Disaster

Contact Info

Product

Resources

About