Cytogenetic analysis of interspecific sunflower hybrids and molecular evaluation of their progeny

Binsfeld, P. C.; Wingender, Ruth; Schnabl, Heide

doi:10.1007/s001220000517

Cited by 19 publications

(15 citation statements)

References 15 publications

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“…Similar meiotic abnormalities were also present in cells of somatic hybrids in other higher plants, such as potato (Pijnacker et al 1992, Conicella et al 1997, sunflower (Binsfeld et al 2001) and tomato (Gavrilenko et al 2001), etc. Sweet orange and lemon, as well as sweet orange and lime, are closely related and they are sexually compatible, without any crossing barriers. The high percentage of univalent and multivalent chromosomes exhibited in PMCs of HR and KV, could be due either to high homology or genetic recombination between the genomes of both parents.…”

Section: Discussionmentioning

confidence: 87%

Cytogenetic analysis of two interspecific Citrus allotetraploid somatic hybrids and their diploid fusion parents

Chen

Guo

et al. 2004

Plant Breeding

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Microsporogenesis of two interspecific allotetraploid somatic hybrids, i.e. ÔHamlinÕ sweet orange + ÔRough LemonÕ (HR) and ÔKeyÕ lime + ÔValenciaÕ sweet orange (KV), was examined and compared with their corresponding diploid fusion parents. Meiotic analysis revealed that only 13.2 and 18.0% of the meiocytes exhibited normal tetrad formation in HR and KV somatic hybrid plants, respectively, and irregular chromosome behaviour with univalent or multivalent pairing occurred. Moreover, meiotic abnormalities such as chromosome bridges, chromosomes orientated away from the equatorial plate, especially lagging chromosomes, which resulted in different sizes of pollen grain, were frequently observed in both somatic hybrids. As expected, the percentage of chromosome abnormalities was much lower in their corresponding diploid fusion parents. HR and KV had 15.4 and 7.2% pollen germinability, respectively, intermediate between their corresponding fusion parents. The meiotic behaviour of these somatic hybrids provided valuable information for their practical utilization in a citrus breeding programme.

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Section: Discussionmentioning

confidence: 87%

Cytogenetic analysis of two interspecific Citrus allotetraploid somatic hybrids and their diploid fusion parents

Chen

Guo

et al. 2004

Plant Breeding

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“…4b; P<0.01). Variation in the chromosome number in somatic hybrids is a normal phenomenon, especially in the case of spontaneous chromosome elimination in somatic hybrids between distantly related species (Binsfeld et al 2001;Miranda et al 1997). …”

Section: Chromosome Analysismentioning

confidence: 98%

Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion

et al. 2004

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Symmetric somatic hybrid plants between Gossypium hirsutum Coker 201 and G. klotzschianum were obtained through electrofusion. The fusion products were cultured in KM8P medium supplemented with 2.685 microM alpha-naphthaleneacetic acid and 0.465 microM kinetin, and the regenerated plants were morphologically, genetically, and cytologically characterized. Nuclear-DNA flow cytometric analysis revealed that the plants tested (31 of 40) had a relative DNA content close to the total DNA contents of the two parents. Subsequent genome DNA analysis using random amplified polymorphic DNA markers revealed 16 of 18 plants were true somatic hybrids. Cytological investigation of the metaphase root-tip cells of seven hybrids revealed there were 72-81 chromosomes in the hybrids, a value close to the expected 78 chromosomes. The morphology of the hybrids was distinct from that of the parents and from that of the regenerants from protoplasts of Coker 201. Somatic hybridization represents a potent and novel tool for transferring genomes of wild cottons containing economically important traits to cultivars in breeding programs. This is the first report on the regeneration of somatic hybrids via protoplast fusion in cotton.

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“…2B-D). Variation of chromosome number including spontaneous chromosome elimination in somatic hybrids is a normal phenomenon, especially arising from distantly related species (Binsfeld et al, 2001;Miranda et al, 1997;Sun et al, 2004). It was very difficult to be precise in counting the cotton chromosomes because of the large numbers involved.…”

Section: Assessment Of Somatic Hybridsmentioning

confidence: 99%

Somatic hybrids between Gossypium hirsutum L. (4×) and G. davidsonii Kellog (2×) produced by protoplast fusion

et al. 2006

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Somatic hybrids were obtained from electrofused protoplasts derived from embryogenic suspension cultures of tetraploid cotton (G. hirsutum L. cv. Coker 201) and embryogenic callus of diploid wild cotton G. davidsonii. The regenerants were initially identified as hybrids by RAPD (random amplified polymorphic DNA) analysis. Subsequently, observation on chromosome counting, morphology and SSR (simple sequence repeat) confirmed the hybrid status. Cytological investigation of the metaphase root-tip cells of the regenerated plants revealed there were 74 to 84 chromosomes in the plants, close to the expected 78 chromosomes. SSR analysis revealed the regenerated plants contained specific genomic fragments from both fusion partners, further confirmed their hybridity. The morphology of the plants was intermediate between the two fusion partners. The regenerants were difficult to develop into mature plants because their roots browned and they wilted from the stem apex before forming 3 to 5 true leaves. The hybrid plants were transferred to soil by grafting in vitro onto rootstocks.

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Cytogenetic analysis of interspecific sunflower hybrids and molecular evaluation of their progeny

Cited by 19 publications

References 15 publications

Cytogenetic analysis of two interspecific Citrus allotetraploid somatic hybrids and their diploid fusion parents

Cytogenetic analysis of two interspecific Citrus allotetraploid somatic hybrids and their diploid fusion parents

Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion

Somatic hybrids between Gossypium hirsutum L. (4×) and G. davidsonii Kellog (2×) produced by protoplast fusion

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