Cytogenetic analysis of human oocytes after subzonal insemination

Selva, J.; Wolf, Jean Philippe; Rince, P.; Rodrígues, D.; Frydman, René; Jouannet, Pierre

doi:10.1002/pd.1970130502

Cited by 15 publications

(4 citation statements)

References 15 publications

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“…It appears from cytoanalysis studies by Dozortsev et al (1994) that most unfertilized oocytes after ICSI show evidence of intact spermatozoa in the cytoplasm. This confirms the previous studies of Selva et al (1993) and Urner et al (1993) which proposed that after SUZI, a relatively high number of spermatozoa penetrate the oocyte without attaining head decondensation.…”

Section: Discussionsupporting

confidence: 92%

Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema

Vanderzwalmen¹,

Bertin²,

Lejeune³

et al. 1996

Human Reproduction

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A total of 740 cycles of intracytoplasmic sperm injection (ICSI) were performed: 625 cycles when < 6 x 10(5) total motile spermatozoa were harvestable from the ejaculate and 115 cycles in cases with a history of previous fertilization failure after classic in-vitro fertilization or subzonal sperm injection. An average of two pronuclei were observed in 63% of the injected oocytes, allowing 725 transfers of a maximum of three embryos (98%). Of 214 pregnancies initiated, 179 were established (25% of ICSI attempts). Because the fertilization rates from our initial 80 ICSI cycles were 2-fold less than those achieved previously, we changed the injection procedure and analysed, in 740 ICSI attempts, the importance of interfering technical factors and how to establish a successful ICSI programme. A remarkable change in the fertilization rate up to 68% (595 cycles) occurred when two steps in the injection procedure were performed well, i.e. immobilization of the spermatozoon and placement of the spermatozoon into the ooplasm after cytoplasmic aspiration into the pipette until oolema rupture. This immobilization, by touching the tail with the pipette, is mandatory for increasing the percentage of fertilization, even with totally non-motile spermatozoa (41%). Because aspiration of the cytoplasm is an invasive part of the ICSI procedure and influences the quality of the embryos, it is essential to reduce the amount of cytoplasm drawn into the pipette. This could be attained by using a spikeless injection pipette with the smallest possible internal diameter.

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Section: Discussionsupporting

confidence: 92%

Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema

Vanderzwalmen¹,

Bertin²,

Lejeune³

et al. 1996

Human Reproduction

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“…This suggests that CMA3 assay not only detects excess histone, but also reveals absence of protamine where there is no histone present. Sakkas (31) and Selva (39) reported that a high percentage of injected or penetrated oocyte that fails to progress to prouclear stage contains condense sperm nuclei in ICSI and SUZI, respectively. One possible explanation of this could be the fact that CMA3 positive spermatozoa after entering the oocyte via zona pellucida or direct injection showed premature chromosomal condensation due to the active maturation promoting factor (MPF) of oocyte (40) or the other possible explanation could be that the absence of protamine on specific region of DNA might prevent decondensation in the other regions.…”

Section: Discussionmentioning

confidence: 99%

Untitled

Nasr‐Esfahani

Razavi

Mardani

2001

Journal of Assisted Reproduction and Genetics

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“…Flaherty et al [8] showed a complete failure of sperm head decondensation in ~11% of the unfertilized metaphase oocytes, whereas Dozortzev et al [6] reported a much higher corresponding percentage (38%). Selva et al [18] reported that 76% of the penetrated oocytes that failed to progress to the pronuclear stage contained sperm nuclei.…”

mentioning

confidence: 99%

Chromatin Decondensation of Human Sperm in Vitro and Its Relation to Fertilization Rate After Icsi

Hammadeh

Strehler

Zeginiadou

et al. 2001

Archives of Andrology

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The inability of sperm chromatin to decondense has been implicated in the failure of fertilization, This study was undertaken to identify the relationship between sperm chromatin decondensation in vitro after incubation with follicular fluid at various points in time and fertilization or pregnancy rates after intracytoplasmic sperm injection. Moreover, an attempt was made to determine whether this test could be used as a predictive test for the outcome of ICSI. Thirty-two infertile couples undergoing ICSI therapy were included in this prospective study. One milliter of semen from each sample was mixed with 1 mL of follicular fluid obtained from ICSI patients at the time of oocyte retrieval and incubated for 24 h. Many smears were made directly after semen liquefaction at the following time intervals: 30, 60, and 120 min and 24 h. Chromatin decondensation was evaluated with acridine orange staining. The mean percentage of uncondensed chromatin of spermatozoa in the native semen samples was 25 +/- 18.3%, which increased within 24 h to 91 +/- 9.5%. On the other hand, the fertilization and ongoing pregnancy rates were 64 +/- 21.7% and 20%, respectively. However, no correlations were found between chromatin decondensation at various point of time (30, 60, and 120 min and 24 h) and fertilization rate. No correlation was shown between the chromatin decondensation and sperm counts in the ejaculate. morphology, or the percentage of condensed chromatin. In light of this study, chromatin decondensation in vitro cannot be recommended for predicting the fertilization potential of spermatozoa and pregnancy rates in the ICSI program. Further research is necessary, especially in cases where ICSI is being considered as a therapeutic option.

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Cytogenetic analysis of human oocytes after subzonal insemination

Cited by 15 publications

References 15 publications

Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema

Two essential steps for a successful intracytoplasmic sperm injection: injection of immobilized spermatozoa after rupture of the oolema

Untitled

Chromatin Decondensation of Human Sperm in Vitro and Its Relation to Fertilization Rate After Icsi

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