Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes)

Hashimoto, Diogo Teruo; Voltolin, Tatiana Aparecida; Paes, Ana Danyelle Noitel Valim de Arruda; Foresti, Fausto; Bortolozzi, Jehud; Porto‐Foresti, Fábio

doi:10.3897/compcytogen.v6i2.1769

Cited by 13 publications

(11 citation statements)

References 32 publications

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“…sanctaefilomenae harbour B chromosomes [ 21 – 25 ]. Since their first description, several authors have proposed the occurrence of three B types in this species, distinguishable by their C-banding patterns [ 21 , 24 ]. Recently, it was reported that some B chromosomes in this species carry 18S rDNA clusters, while others do not [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…B chromosomes containing 18S rRNA genes have been described in different fish species [ 9 , 62 ], but their activity has only been demonstrated for the euchromatic variant (B 1 ) in M . sanctaefilomenae [ 24 ]. Several other examples of NOR-bearing B chromosomes have been described in different organisms reviewed in [ 61 ], and there is no general trend between the C-banding response and NOR activity of the B chromosomes.…”

Section: Discussionmentioning

confidence: 99%

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Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae)

et al. 2016

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B chromosomes constitute a heterogeneous mixture of genomic parasites that are sometimes derived intraspecifically from the standard genome of the host species, but result from interspecific hybridization in other cases. The mode of origin determines the DNA content, with the B chromosomes showing high similarity with the A genome in the first case, but presenting higher similarity with a different species in the second. The characid fish Moenkhausia sanctaefilomenae harbours highly invasive B chromosomes, which are present in all populations analyzed to date in the Parana and Tietê rivers. To investigate the origin of these B chromosomes, we analyzed two natural populations: one carrying B chromosomes and the other lacking them, using a combination of molecular cytogenetic techniques, nucleotide sequence analysis and high-throughput sequencing (Illumina HiSeq2000). Our results showed that i) B chromosomes have not yet reached the Paranapanema River basin; ii) B chromosomes are mitotically unstable; iii) there are two types of B chromosomes, the most frequent of which is lightly C-banded (similar to euchromatin in A chromosomes) (B1), while the other is darkly C-banded (heterochromatin-like) (B2); iv) the two B types contain the same tandem repeat DNA sequences (18S ribosomal DNA, H3 histone genes, MS3 and MS7 satellite DNA), with a higher content of 18S rDNA in the heterochromatic variant; v) all of these repetitive DNAs are present together only in the paracentromeric region of autosome pair no. 6, suggesting that the B chromosomes are derived from this A chromosome; vi) the two B chromosome variants show MS3 sequences that are highly divergent from each other and from the 0B genome, although the B2-derived sequences exhibit higher similarity with the 0B genome (this suggests an independent origin of the two B variants, with the less frequent, B2 type presumably being younger); and vii) the dN/dS ratio for the H3.2 histone gene is almost 4–6 times higher for B chromosomes than for A chromosome sequences, suggesting that purifying selection is relaxed for the DNA sequences located on the B chromosomes, presumably because they are mostly inactive.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae)

et al. 2016

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“…The B chromosomes described here are primarily composed of heterochromatic sequences, specifically the AATAT satellite repeat. Although these chromosomes appear to possess centromeres, like many B chromosomes they are mitotically unstable ( Voltolin et al 2012 ).…”

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Discovery of SupernumeraryBChromosomes inDrosophila melanogaster

et al. 2014

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B chromosomes are small, heterochromatic chromosomes that are transmitted in a non-Mendelian manner. We have identified a stock of Drosophila melanogaster that recently (within the last decade) acquired an average of 10 B chromosomes per fly. These B chromosomes are transmitted by both males and females and can be maintained for multiple generations in a wild-type genetic background despite the fact that they cause high levels of 4th chromosome meiotic nondisjunction in females. Most curiously, these B chromosomes are mitotically unstable, suggesting either the absence of critical chromosomal sites or the inability of the meiotic or mitotic systems to cope with many additional chromosomes. These B chromosomes also contain centromeres and are primarily composed of the heterochromatic AATAT satellite sequence. Although the AATAT sequence comprises the majority of the 4th chromosome heterochromatin, the B chromosomes lack most, if not all, 4th chromosome euchromatin. Presumably as a consequence of their heterochromatic content, these B chromosomes significantly modify position-effect variegation in two separate reporter systems, acting as enhancers of variegation in one case and suppressors in the other. The identification of B chromosomes in a genetically tractable organism like D. melanogaster will facilitate studies of chromosome evolution and the analysis of the mechanisms by which meiotic and mitotic processes cope with additional chromosomes.

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“…The B chromosomes have been described in many neotropical fish groups (see, for example, Maistro et al 1992, Oliveira et al 1997, Maistro et al 2000, Torres-Mariano and Morelli 2008, Ferreira-Neto et al 2012, Hashimoto et al 2012, Silva et al 2014). The occurrence of this type of chromosome among individuals of a population can be sporadic or commonly found for many individuals, and high frequency can be shown between them.…”

Section: Introductionmentioning

confidence: 99%

“…The occurrence of this type of chromosome among individuals of a population can be sporadic or commonly found for many individuals, and high frequency can be shown between them. It is also possible to find variations regarding to morphology, size, number and pattern of heterochromatin in the B chromosomes (Maistro et al 1992, Venere et al 1999, Cavallaro et al 2000, Fernandes and Martins-Santos 2005, Artoni et al 2006, Hashimoto et al 2012, Barbosa et al 2015). …”

Section: Introductionmentioning

confidence: 99%

First description of B chromosomes in the Hyphessobrycon (Characiformes, Characidae) genus: a hypothesis for the extra element of Hyphessobrycon eques Steindachner, 1882

Piscor¹,

Parise-Maltempi²

2015

CCG

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The Hyphessobrycon are allocated in the incertae sedis group of the Characidae family, one of the genera with more species of the group. The chromosomes of some species of Hyphessobrycon are known, and the diploid number most common for genus is 2n = 50 chromosomes. The aims of this study were to examine the karyotype macrostructure in the Hyphessobrycon eques Steindachner, 1882, and show a new origin hypothesis for B chromosomes. The diploid number observed for Hyphessobrycon eques was 2n = 52 chromosomes, and a karyotype formulae of 12m + 18sm + 8st + 14a, with FN (fundamental number) = 90 for both sexes. Only two females showed one B chromosome. The heterochromatin was observed mainly on centromeric regions, and in the long arm of the B chromosome. In this paper, the relationship of the B chromosome of Hyphessobrycon eques with an occasional chromosome rearrangement was discussed.

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Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes)

Cited by 13 publications

References 32 publications

Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae)

Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae)

Discovery of SupernumeraryBChromosomes inDrosophila melanogaster

First description of B chromosomes in the Hyphessobrycon (Characiformes, Characidae) genus: a hypothesis for the extra element of Hyphessobrycon eques Steindachner, 1882

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