Cytogenetic analysis of a hybrid, Glyptotendipes pallens Mg. ×Glyptotendipes glaucus Mg. (Diptera, Chironomidae): evolutionary considerations

Michailova, Paraskeva

doi:10.1111/j.1439-0469.1998.tb00841.x

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…This result does not affect the status of the species since the capability of closely related taxa to hybridize is regarded as a plesiomorphic state that occurs among insects (e.g. [ 62 , 63 ]) and it has been demonstrated in Chironomidae (e.g., [ 64 , 65 ]). Crucially, the possible event of hybridization occurred at a very small glacier (area < 1 km 2 ), the Trobio, in the Orobian Alps, where the limited living and breeding habitats improve the probability of contact amongst organisms.…”

Section: Resultsmentioning

confidence: 99%

Integrated Taxonomy and DNA Barcoding of Alpine Midges (Diptera: Chironomidae)

et al. 2016

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Rapid and efficient DNA-based tools are recommended for the evaluation of the insect biodiversity of high-altitude streams. In the present study, focused principally on larvae of the genus Diamesa Meigen 1835 (Diptera: Chironomidae), the congruence between morphological/molecular delimitation of species as well as performances in taxonomic assignments were evaluated. A fragment of the mitochondrial cox1 gene was obtained from 112 larvae, pupae and adults (Diamesinae, Orthocladiinae and Tanypodinae) that were collected in different mountain regions of the Alps and Apennines. On the basis of morphological characters 102 specimens were attributed to 16 species, and the remaining ten specimens were identified to the genus level. Molecular species delimitation was performed using: i) distance-based Automatic Barcode Gap Discovery (ABGD), with no a priori assumptions on species identification; and ii) coalescent tree-based approaches as the Generalized Mixed Yule Coalescent model, its Bayesian implementation and Bayesian Poisson Tree Processes. The ABGD analysis, estimating an optimal intra/interspecific nucleotide distance threshold of 0.7%-1.4%, identified 23 putative species; the tree-based approaches, identified between 25–26 entities, provided nearly identical results. All species belonging to zernyi, steinboecki, latitarsis, bertrami, dampfi and incallida groups, as well as outgroup species, are recovered as separate entities, perfectly matching the identified morphospecies. In contrast, within the cinerella group, cases of discrepancy arose: i) the two morphologically separate species D. cinerella and D. tonsa are neither monophyletic nor diagnosable exhibiting low values of between-taxa nucleotide mean divergence (0.94%); ii) few cases of larvae morphological misidentification were observed. Head capsule color is confirmed to be a valid character able to discriminate larvae of D. zernyi, D. tonsa and D. cinerella, but it is here better defined as a color gradient between the setae submenti and genal setae. DNA barcodes performances were high: average accuracy was ~89% and precision of ~99%. On the basis of the present data, we can thus conclude that molecular identification represents a promising tool that could be effectively adopted in evaluating biodiversity of high-altitude streams.

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Section: Resultsmentioning

confidence: 99%

Integrated Taxonomy and DNA Barcoding of Alpine Midges (Diptera: Chironomidae)

et al. 2016

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“…(1983), Kiknadze et al. (1991) for genus Chironomus and the maps of Walter (1973), Michailova (1989a,b), Michailova (1998), Michailova and Contreras‐Lichtenberg (1995) for genus Glyptotendipes. Kiefferulus tendipediformis chromosomes were identified and the hybridization signal was established using the photographic map of Michailova et al.…”

Section: Methodsmentioning

confidence: 99%

In situlocalization of the evolutionary conserved Cpy/Cty gene in the subfamily Chironominae (Chironomidae, Diptera): establishment of chromosomal homologies

Michailova

Krastanov

Hankeln

et al. 2009

Journal of Zoological Systematics and Evolutionary Research

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The homologous sites on the salivary gland chromosomes of 13 species from three genera: Chironomus, Glyptotendipes, Kiefferulus have been mapped by means of fluorescent in situ hybridization using the evolutionary conserved gene Cpy⁄Cty (clone Cla1.1). In all species of genus Chironomus and genus Kiefferulus, the Cty⁄Cpy gene is located on arm F of chromosome EF. The relocation of the gene among the species of genus Chironomus can be done by simple or complex homozygous inversions which occurred during the divergent evolution of the chromosome of the species. In the genus Glyptotendipes, the Cty⁄Cpy gene was localized in arm E of chromosome EF. Since the banding patterns of salivary gland chromosomes between genus Chironomus and genus Glyptotendipes cannot be compared directly, in situ hybridization with clone of conservative gene was performed to be established some homologous chromosomes. The results obtained indicate that the chromosome arm F of Chironomus and chromosome arm E of Glyptotendipes may be homologous.

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Cytogenetic analysis of a hybrid, Glyptotendipes pallens Mg. ×Glyptotendipes glaucus Mg. (Diptera, Chironomidae): evolutionary considerations

Cited by 2 publications

References 5 publications

Integrated Taxonomy and DNA Barcoding of Alpine Midges (Diptera: Chironomidae)

Integrated Taxonomy and DNA Barcoding of Alpine Midges (Diptera: Chironomidae)

In situlocalization of the evolutionary conserved Cpy/Cty gene in the subfamily Chironominae (Chironomidae, Diptera): establishment of chromosomal homologies

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