Cytogenetic analysis by chromosome painting

Carter, Nigel P.

doi:10.1002/cyto.990180103

Cited by 60 publications

(32 citation statements)

References 42 publications

(23 reference statements)

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“…Chromosome microdissection is an established technique for generation of painting probes of regular chromosomes or rearranged complex marker chromosomes from human or animal metaphase spreads and of probes of nuclei from tumor tissue sections for comparative genomic hybridization (CGH) (Carter, 1994;Sharma and Sharma, 2001;Teixeira, 2002). In A. lucidus, a probe of a specific K type was made by microdissection of ten K4s of freshly prepared spermatogonial metaphases for reverse painting onto polytene salivary gland Ss to identify the S-homologous sections of K4 (Staiber and Schiffkowski, 2000).…”

Section: Discussionmentioning

confidence: 99%

Isolation of a new germ line-specific repetitive DNA family in <i>Acricotopus</i> by microdissection of polytenized germ line-limited chromosome sections from a permanent larval salivary gland preparation

Staiber

2002

Cytogenet Genome Res

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In Acricotopus lucidus (Diptera, Chironomidae) the germ line-limited chromosomes (Ks) have developed from the soma chromosomes (Ss) by endoreduplication, rearrangements and accumulation of germ line-specific repetitive sequences. For molecular analysis of specific small K sections, microdissection of metaphase Ks generally yields very limited amounts of DNA. In this study, K-specific DNA was microdissected from defined polytenized K sections of X-ray induced K-S-rearrangements of permanent salivary gland chromosome preparations and was then amplified by DOP-PCR. A new germ line-specific tandem repetitive DNA family was isolated by this way from a heterochromatic K segment, characterized and localized on the Ks by FISH. The repetitive elements are related to sequences of earlier described K-specific tandem repetitive DNA families in A. lucidus, but are located mainly in terminal heterochromatin bands of the two largest Ks and only to a limited degree in the paracentromeric K heterochromatin. This demonstrates that a collection of permanent preparations of K-S-rearrangements with polytenized heterochromatic and S-homologous K sections of A. lucidus can be used as a source for obtaining K sequences of defined K parts to investigate the molecular evolution of the Ks.

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Section: Discussionmentioning

confidence: 99%

Isolation of a new germ line-specific repetitive DNA family in <i>Acricotopus</i> by microdissection of polytenized germ line-limited chromosome sections from a permanent larval salivary gland preparation

Staiber

2002

Cytogenet Genome Res

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“…Human paint probes were generated from flow-sorted chromosomes following a previously described method (Carter, 1994). For reciprocal hybridisation of human paints onto dog chromosomes, 150 ng of each biotinylated paint probe were mixed separately with 10 Ìg of sonicated dog genomic DNA in 15 Ìl of hybridisation buffer.…”

Section: Hybridisation Of Human Chromosome Paints Onto Dog Chromosomesmentioning

confidence: 99%

Zoo-FISH analysis of dog chromosome 5: identification of conserved synteny with human and cat chromosomes

Thomas

Breen

Langford

et al. 1999

Cytogenet Genome Res

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Conserved segments of synteny between the human genome and chromosome 5 (CFA 5) of the domestic dog (Canis familiaris) have been identified by reciprocal chromosome painting analysis. A CFA 5 paint probe was applied to human metaphase spreads, revealing distinct hybridisation sites on human (HSA) chromosomes 1, 11, 16, and 17. Paint probes for these human chromosomes were then hybridised to dog metaphase spreads, identifying the regions of CFA 5 with which homology is shared with the corresponding human chromosome. Application of the CFA 5 paint probe to metaphase spreads of the domestic cat (Felis catus, FCA) demonstrated hybridisation to cat chromosomes C1, D1, E1, and E2. Dog PCR primers for type 1 markers known to lie in the corresponding regions of HSA 11, 16, and 17 were used to isolate dog BAC clones representing four genes. Fluorescence in situ hybridisation analysis confirmed their localisation to CFA 5 and suggested that two of the conserved segments lie in opposing orientations on CFA 5, compared to the human chromosome concerned. A third segment appears to lie in the same orientation on both human and dog chromosomes. No suitable gene markers were available for analysis of the fourth segment. The significance of these findings is discussed with reference to current and future dog genome mapping efforts.

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“…The majority of these studies employed human whole chromosome painting probes. However, the combination of degenerate oligonucleotide-primed PCR (DOP-PCR) and chromosome sorting by bivariate flow cytometry facilitated the generation of high-quality chromosome painting probes for an increasing number of species (Carter 1994;Ferguson-Smith 1997).…”

Section: Introductionmentioning

confidence: 99%

Towards the delineation of the ancestral eutherian genome organization: comparative genome maps of human and the African elephant (Loxodonta africana) generated by chromosome painting

Frönicke

Wienberg²,

Stone

et al. 2003

Proc. R. Soc. Lond. B

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This study presents a whole-genome comparison of human and a representative of the Afrotherian clade, the African elephant, generated by reciprocal Zoo-FISH. An analysis of Afrotheria genomes is of special interest, because recent DNA sequence comparisons identify them as the oldest placental mammalian clade. Complete sets of whole-chromosome specific painting probes for the African elephant and human were constructed by degenerate oligonucleotide-primed PCR amplification of flow-sorted chromosomes. Comparative genome maps are presented based on their hybridization patterns. These maps show that the elephant has a moderately rearranged chromosome complement when compared to humans. The human paint probes identified 53 evolutionary conserved segments on the 27 autosomal elephant chromosomes and the X chromosome. Reciprocal experiments with elephant probes delineated 68 conserved segments in the human genome. The comparison with a recent aardvark and elephant Zoo-FISH study delineates new chromosomal traits which link the two Afrotherian species phylogenetically. In the absence of any morphological evidence the chromosome painting data offer the first non-DNA sequence support for an Afrotherian clade. The comparative human and elephant genome maps provide new insights into the karyotype organization of the proto-afrotherian, the ancestor of extant placental mammals, which most probably consisted of 2n = 46 chromosomes.

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Cytogenetic analysis by chromosome painting

Cited by 60 publications

References 42 publications

Isolation of a new germ line-specific repetitive DNA family in <i>Acricotopus</i> by microdissection of polytenized germ line-limited chromosome sections from a permanent larval salivary gland preparation

Isolation of a new germ line-specific repetitive DNA family in <i>Acricotopus</i> by microdissection of polytenized germ line-limited chromosome sections from a permanent larval salivary gland preparation

Zoo-FISH analysis of dog chromosome 5: identification of conserved synteny with human and cat chromosomes

Towards the delineation of the ancestral eutherian genome organization: comparative genome maps of human and the African elephant (Loxodonta africana) generated by chromosome painting

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