Cytogenetic analysis and developmental capacity of normal and abnormal embryos after IVF

Plachot, M.; Mandelbaum, J.; Junca, A. M.; Grouchy, J. de; Salat-Baroux, J.; Cohen, Jacques

doi:10.1093/humrep/4.suppl_1.99

Cited by 77 publications

(29 citation statements)

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“…Tripronuclear embryo formation has been reported in 5-6% of human IVF programs regardless of the intracytoplasmic sperm injection (ICSI) technique used [1][2][3][4][5][6]. The authors of the present study found that the incidence of tripronuclear zygotes was 3.5% in an ICSI program at the IVF center of Seoul National University Hospital from 1995 to 1998.…”

Section: Introductionmentioning

confidence: 54%

Chromosomal Constitution of Embryos Derived from Tripronuclear Zygotes Studied by Fluorescence in situ Hybridization Using Probes for Chromosomes 4, 13, 18, 21, X, and Y

Pang¹,

Jee

Kim

et al. 2005

Gynecol Obstet Invest

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This study was designed to assess the chromosomal constitution and segregation patterns of cleaving embryos derived from tripronuclear zygotes. Thirty-two embryos obtained from 19 conventional IVF patients were analyzed by fluorescence in situ hybridization (FISH) using probes for chromosomes 4, 13, 18, 21, X, and Y. Sixteen embryos (50.0%) exhibited uniform, non-mosaic patterns. These embryos showed pure triploid (n = 7), pure diploid (n = 7), or pure haploid (n = 2). The remaining 16 embryos showed mosaic patterns; 1 was triploid-diploid mosaics, 9 were diploid-haploid, and 4 were haploid only. Autosomal aneuploidy occurred in 2 embryos showing a triploid complement. The sex chromosomal constituent XXX:XXY:XYY was 3:4:2 in embryos showing a pure triploid complement (including 2 embryos with aneuploidy). This ratio was not significantly different from the expected 1:2:1 (p = 0.96). Pure triploid was found in 41.7% of 2-cell embryos, but no triploid complement was found in 3-cell embryos. The present study also supports the diandric origin of tripronuclear zygotes in the conventional IVF, and, to our knowledge, is the first study to use simultaneous six-color FISH for chromosomes 4, 13, 18, 21, X, and Y in human embryos. However, no additive information was obtained about chromosome 4.

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Section: Introductionmentioning

confidence: 54%

Chromosomal Constitution of Embryos Derived from Tripronuclear Zygotes Studied by Fluorescence in situ Hybridization Using Probes for Chromosomes 4, 13, 18, 21, X, and Y

Pang¹,

Jee

Kim

et al. 2005

Gynecol Obstet Invest

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“…In prior studies on the ploidy status of embryos developing from 1PN oocytes, the proportional incidence of haploidy and diploidy varied [4,[9][10][11][12] . In earlier cytogenetic investigations, fluorescent in situ hybridization (FISH) with sex chromosome probes were used to determine if 1PN oocytes had been fertilized.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Sex Chromosomes of Human Embryos Arising from Monopronucleus Zygotes in in vitro Fertilization and Intracytoplasmic Sperm Injection Cycles of Chinese Women

Yan

Shi

et al. 2009

Gynecol Obstet Invest

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Objectives: To examine abnormal fertilization phenomena in blastomeres of embryos generated from conventional IVF and ICSI displaying a single pronucleus at the zygote stage. Methods: 132 embryos from monopronuclear zygotes (1PN) generated from conventional IVF and ICSI were examined by FISH (fluorescent in situ hybridization) with X, Y dual color centromere probes. Results: In the embryos that were obtained from conventional IVF, the percentage of diploid, monoploid and mosaic sex chromosome were 54.35, 23.91 and 21.74%, respectively. In the embryos from 1PN zygotes derived from ICSI, the percentage of diploid, monoploid and mosaic sex chromosomes were 31.51, 31.51 and 36.99%, respectively. For monoploid embryos, the ratio of XO was significantly higher than that of YO in both conventional IVF and ICSI groups. Conclusion: The results demonstrated that the majority of embryos derived from 1PN zygotes generated with conventional IVF or ICSI have a high incidence of aneuploidy. Furthermore, the sex chromosome diploid ratio of embryos from 1PN zygotes derived from conventional IVF was significantly higher than those generated from ICSI procedure in Chinese women. Nevertheless, the high proportion of aneuploid embryos suggests that such embryos should not be utilized for embryo transfer.

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“…The data are derived from two sources, viz. the technically difficult karyotyping that may provide information on all the chromosomes but from a limited number of cells per embryo (Angell 1989, Plachot et al 1989Zenzes and Casper 1992;Jamieson et al 1994) and, more recently, from fluorescent in situ hybridisation (FISH) with chromosome-specific DNA probes that give signals detectable in the interphase nucleus (Pinkel et al 1986). The advantage of the FISH technique is that results are obtained for all the cells of an embryo but there is a limit to the number of probes that can be used simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Multicolour FISH detects frequent chromosomal mosaicism and chaotic division in normal preimplantation embryos from fertile patients

et al. 1997

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We have used multicolour fluorescent in situ hybridisation (FISH) with DNA probes for chromosomes X, Y and l to analyse spare untransferred cleavage-stage embryos after preimplantation diagnosis to avoid X-linked disease. In total, 93 morphologically normal embryos were available from seven patients (six of proven fertility) who had undergone fourteen in vitro fertilisation (IVF) cycles. The chromosome patterns observed were classified into four groups; normal, abnormal (non-mosaic), mosaic and chaotic (uncontrolled division). Approximately half of the embryos were normal for the chromosomes tested. Two embryos only were aneuploid (non-mosaic) throughout but, after excluding those showing chaotic division, 30% were considered to be chromosomal mosaics. Of these, a minority had arisen because of mitotic non-disjunction or chromosome loss or gain, whereas the majority were ploidy mosaics, with haploidy being the most common. The occurrence of chaotically dividing embryos was strongly patient-related, i.e. some patients had 'chaotic' embryos in repeated cycles, whereas other patients were completely free of this type of anomaly. 'Chaotic' embryos are unlikely to progress beyond implantation. These findings have important implications both for routine IVF and preimplantation genetic diagnosis.

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Cytogenetic analysis and developmental capacity of normal and abnormal embryos after IVF

Cited by 77 publications

References 0 publications

Chromosomal Constitution of Embryos Derived from Tripronuclear Zygotes Studied by Fluorescence in situ Hybridization Using Probes for Chromosomes 4, 13, 18, 21, X, and Y

Chromosomal Constitution of Embryos Derived from Tripronuclear Zygotes Studied by Fluorescence in situ Hybridization Using Probes for Chromosomes 4, 13, 18, 21, X, and Y

Assessment of Sex Chromosomes of Human Embryos Arising from Monopronucleus Zygotes in in vitro Fertilization and Intracytoplasmic Sperm Injection Cycles of Chinese Women

Multicolour FISH detects frequent chromosomal mosaicism and chaotic division in normal preimplantation embryos from fertile patients

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