Cytogenetic abnormalities in aquatic plant Elodea canadensis in anthropogenic contamination zone of Yenisei River

Medvedeva, M. Yu.; Bolsunovsky, A. Ya.; Зотина, Т. А.

doi:10.1134/s1995425514040088

Cited by 14 publications

(16 citation statements)

References 10 publications

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“…В данном исследовании отстающие хромосомы и их фрагменты подсчитывались суммарно, а мосты разделялись на два типа -одиночные и множе-ственные. В дальнейшей работе на корнях элодеи прово-дился подсчет одиночных и двойных фрагментов в клет-ках, при этом идентифицировать фрагменты можно было только субъективно -по относительному их размеру и форме (целые хромосомы -двуплечие, фрагменты -палочковидные) [26]. Кольцевые хромосомы были зарегистрированы в пробах элодеи из реки Енисей -в трех исследуемых районах (Красноярск-2, Атаманово, Стрелка) на стадии метафазы и только в ближней зоне ГХК (Атаманово) -на стадии анателофазы.…”

Section: Discussionunclassified

“…Удачный, располо-жен в 14 км выше по течению реки от г. Красноярска (контрольный район), в 94 км от ГХК; Красноярск-2 -район расположен в центре г. Красноярска (о. Татышев, 0 км), в 80 км от ГХК; Красноярск-3 -район с. Есаулово, рас-положен на 43 км ниже по течению от г. Красноярска и на 37 км выше ГХК. Два из трех вышеупомянутых районов реки Енисей подвержены химическо-му загрязнению от промышленно-коммунального комплекса г. Красноярска и его окрестностей [26]. В предыдущих исследованиях [26] было выявлено повышенное содержание некоторых металлов (Cu, Zn, Pb, Ni) в донных от-ложениях реки ниже г. Красноярска, причем зарегистрированные концен-трации тяжелых металлов превышали пороговый уровень концентрации, при которой токсические эффекты для пресноводных бентосных организмов ма-ловероятны (TEC, threshold effect concentration) [25].…”

Section: материалы и методыunclassified

“…Район отбора Ата-маново расположен на 87 км ниже по течению реки от г. Красноярска и в 7 км от сбросов ГХК. Донные отложения, отобранные в этом районе в 2012 г., со-держали до 5 [26]. Актив-ность техногенных радионуклидов в донных отложени-ях из района Стрелка была в 10 раз выше, чем в пробах донных отложений из района Атаманово [26].…”

Section: материалы и методыunclassified

“…[10,16] и в 2011-2012 гг. [26] были подробно изучены ци-тогенетические характеристики элодеи канадской. Было также показано зна-чительное возрастание частоты цитогенетических аномалий в клетках элодеи, вегетирующей в зоне радиоактивного загрязнения Горно-химического ком-бината Росатома (ГХК, г. Железногорск).…”

Section: Introductionunclassified

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Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

Medvedeva

Bolsunovsky

2016

Ecological genetics

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Background. The spectrum of cytogenetic abnormalities in the apical root meristem of Elodea сanadensis Michx. (elodea) from the Yenisei River was studied depending on the type of anthropogenic pollution. We compared elodea samples from areas with radiation-contaminated sediments (area affected by Rosatom Mining and Chemical Combine, MCC, Zheleznogorsk) and areas with chemical pollution (Krasnoyarsk). Materials and methods. The apical root meristem of elodea was fixed in acetic alcohol (96 % ethanol and glacial acetic acid, 3 : 1) and stained with 1 % aceto-orcein. The cytogenetic analysis of metaphase and ana-telophase elodea cells was carried out with temporal squashed preparations using an Olympus CX31 microscope. Results. At metaphase stage, the predominant types of abnormalities in elodea cells were disoriented chromosomes (up to 9 % of total metaphase cells) and chromosome agglutination (6 %). In the area affected by the MCC an increased content of ring chromosomes in elodea cells was detected, but in terms of frequency of their occurrence no significant differences were revealed between samples from areas with different types of pollution. Among abnormalities at ana-telophase stage, bridges (to 20% of dividing cells) and disoriented chromosomes (up to 8%) dominated. The following abnormalities were also detected: multipolar and asymmetrical mitoses, agglutination and cells with multiple chromosome abnormalities. It was shown that in areas with the highest content of 137Cs in bottom sediments the frequency of cells with bridges and multiple chromosome abnormalities significantly increased as compared to samples from other areas. Conclusion. In the radioactive contamination area of the Yenisei River the spectrum of abnormalities was dominated by chromosome aberrations (bridges, agglutination, chromosome fragmentation) and multiple abnormalities in one and the same cell. In areas with non-radioactive (chemical) contamination of sediments, mitotic spindle irregularities prevailed.

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Section: Discussionunclassified

Section: материалы и методыunclassified

Section: Introductionunclassified

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Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

Medvedeva

Bolsunovsky

2016

Ecological genetics

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“…A dose‐dependent increase in the incidence of chromosome abnormalities was reported in roots of aquatic plants from water bodies in the Chernobyl (Ukraine) exclusion zone . A high percentage of cells with chromosome abnormalities was recorded in roots of aquatic plants sampled in the radioactively and chemically contaminated sediments of the Yenisei River (Siberia, Russia) .…”

Section: Introductionmentioning

confidence: 99%

Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments

Зотина

Трофимова

Medvedeva

et al. 2015

Enviro Toxic and Chemistry

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The toxicity, cytotoxicity, and genotoxicity of bulk sediments from the Yenisei River (Siberia, Russia) were estimated in laboratory bioassays based on several endpoints in the aquatic plant Elodea canadensis. The bottom sediment samples were collected in the Yenisei River upstream and downstream of the sources of chemical and radioactive contamination. The testing revealed different sensitivities of Elodea endpoints to the quality of the bottom sediment: weight of shoots < length of shoots < mitotic index < length of roots < percentage of abnormal cells. The response of the genotoxicity endpoint (percentage of cells with chromosome abnormalities in roots of Elodea) was the highest in sediments with chemical pollution, whereas the highest inhibition of toxicity endpoints (shoot and root length) occurred in sediments with the highest level of radioactive pollution. The extreme response of Elodea endpoints to the quality of certain sediment samples may be regarded as related to the possible presence of unknown toxicants. The results show that E. canadensis can be used as an indicator species in laboratory contact testing of bottom sediment. The responses of shoot and root length growth endpoints of Elodea can be recommended as basic sensitivity indicators of bottom sediment toxicity. Analysis of cells carrying abnormal chromosomes in the apical root meristem of Elodea can be performed optionally in the same test to assess the genotoxicity of sediments.

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Monitoring and assessment of 137Cs and 90Sr radioactive isotopes in the ‘soil – rhizosphere – sedge’ system of the Yenisei River floodplain (near impact zone of Krasnoyarsk MCC, Russia)

Kropacheva

Melgunov

Makarova

et al. 2021

Environ Monit Assess

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Cytogenetic abnormalities in aquatic plant Elodea canadensis in anthropogenic contamination zone of Yenisei River

Cited by 14 publications

References 10 publications

Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments

Monitoring and assessment of 137Cs and 90Sr radioactive isotopes in the ‘soil – rhizosphere – sedge’ system of the Yenisei River floodplain (near impact zone of Krasnoyarsk MCC, Russia)

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