Cytodynamics of the Immune Response in Two Lines of Mice Genetically Selected for "High" and "Low" Antibody Synthesis

Biozzi, G; Stiffel, C; Mouton, D; Bouthillier, Y; Decreusefond, C

doi:10.1084/jem.135.5.1071

Cited by 98 publications

(17 citation statements)

References 37 publications

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“…The mechanism behind these adjuvant effects was sug gested to be interference with phagocyte functions [10,16] which would be in favour of the persistence of the antigen [7,27,38,39], This explanation is in line with observations made in Biozzi mice, suggesting that an impaired phagocyte function was related to high immune-responsiveness and vice versa [4,37], Polyanions have also effects on the complement (C) cascade. The anticomplementary behaviour was shown to concern both the classical and alternative activation pathways which appeared to be inhibited by direct [23,26,36] and indirect [3,9,14,18,29] means.…”

Section: Introductionsupporting

confidence: 73%

Analytic Study of the Differential Anticomplementary Effects of Dextran Sulphate and Heparin in the Assay for the Mouse Alternative Pathway

Klerx¹,

Dijk²,

Maaden³

et al. 1985

Int Arch Allergy Immunol

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In a recent paper, a linkage between immunological adjuvant activity in mice and in vitro anticomplementary (alternative pathway assay) effects was described for different polyanions. This connection was found only if mouse serum was used as a complement (C) source. In order to investigate the possible role of C in adjuvant activity, the differential effects of polyanions on mouse C were studied in detail. For this study, substances with different activity were selected, namely dextran sulphate with strong C-regulatory and immunoadjuvant activities, and heparin, which was weakly anticomplementary and devoid of adjuvant effect. In general, studies of mouse C are complicated by the inavailability of isolation procedures for the C-components involved. This difficulty was circumvented by making use of C5-deficient serum and of the haemolytic activity of mouse membrane attack complexes formed in the fluid phase. With yeast cells as alternative pathway activators it was shown that the effect of heparin on this pathway was restricted to activation of the terminal route. In contrast, dextran sulphate also caused a functional decay of a yeast-bound alternative pathway C5-convertase and interfered with the haemolytic activity of fluid-phase membrane attack complexes as well. Further studies will be needed to decide whether these specific effects of dextran sulphate are related to the immunological adjuvant activity of the substance in mice.

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Section: Introductionsupporting

confidence: 73%

Analytic Study of the Differential Anticomplementary Effects of Dextran Sulphate and Heparin in the Assay for the Mouse Alternative Pathway

Klerx¹,

Dijk²,

Maaden³

et al. 1985

Int Arch Allergy Immunol

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“…In G2m(n,n) patients the IgG2, IgG3 and IgG4 levels were significantly higher than in those lacking this allotype. These pa tients showed high levels also of IgE and IgGl and could be compared with the findings of high-respond ing Biozzi mice [3]. Healthy G2m(n) individuals expressing one or two alleles of G2m(n) have been reported to have higher IgG2 and IgG4 [5,20].…”

Section: Discussionmentioning

confidence: 99%

Gm Allotype Genes and Gene Dosage Affecting Both IgG Subclass and IgE Levels in Atopic Patients

Oxelius

1990

Int Arch Allergy Immunol

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The imbalanced IgG subclass levels of 50 atopic patients with IgE > 600 kU/1 reflected the Gm expression of the patients. IgG1 was significantly increased but only in patients with the phenotypes Gm(ffnn) and Gm(a, a,XXX, XXX)·The typical IgG4 increase was found in the most frequent Gm(ffnn) and Gm(a, f, n,”) phenotypes. Significant increase of IgG2, IgG3 and IgG4 was found in patients homozygous for G2m(n) compared to those lacking this allotype. IgG2 decrease was found in those with the Gm(f, f,XXX,XXX) phenotype and IgG3 decrease in those with the Gm(a, a,XXX,XXX) phenotype. Also the IgE levels were influenced by the Gm allotypes with increased IgE levels in the Glm(ff) patients compared to the G1m(aa) patients.

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“…Hdmmerling et al [6] also reported that the high-responder and lowresponder mice to a branched, multichain polypeptide(T, G)-A--L had about the same number of antigen-binding cells both in the spleens and lymph nodes. According to Biozzi et al [2], the strain difference in antibody production against sheep erythrocytes (SRBC) between two strains of mice was found to be attributable principally to the difference in doubling times of cells engaged in antibody response rather than to different numbers of antigen-specific lymphocytes initiating the immune response. In the present work, the numbers of hemolytic foci and PFC increased comparably (about 13 times) from day 2 to day 3 in SL mice (Tables 1 and 2).…”

Section: Discussionmentioning

confidence: 99%

Immune Response against Hamster Erythrocytes in the Low‐Responder Mouse Strains

Nomoto

Mashiba

Sato

et al. 1974

Japanese Journal of Microbiology

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Patterns of proliferation of antibody-forming cells after an intravenous immunization with hamster erythrocytes (HRBC) were compared in groups of mice possessing different activities of thymus-derived lymphocytes (T cells). 1) Marked differences in the numbers of hemolysin plaque-forming cells (PFC) after HRBC injection were found among the low-and high-responder normal mice and those pretreated with HRBC in complete Freund's adjuvant (CFA) or incomplete adjuvant (IFA), and they appeared to depend primarily upon the different rates of proliferation of antibody-forming cells rather than on the numbers of antigen-specific lymphocytes initiating the antibody response.2) The numbers of hemolytic foci were slightly larger in mice with large numbers of PFC (normal SL mice, the pretreated SL and C57BL/6 mice) than in those with small numbers of PFC (normal C57BL/6 mice). The numbers of hemolytic foci increased at almost the same rate from day 2 to day 3 in both groups, while the numbers of PFC increased more efficiently in mice with large numbers of PFC than in those with small numbers of PFC from day 2 to day 3. Individual hemolytic foci appeared to contain larger numbers of PFC in mice with large total numbers of PFC than in those with small total numbers of PFC.3) The numbers of rosette-forming cells (RFC) were increased by pretreatment with HRBC in CFA and by pretreatment with HRBC in IFA to almost the same extent. Rates of increases in PFC were, however, larger by pretreatment with HRBC in CFA than with HRBC in IFA. These results suggested that the activity of the T cell determined not only the rates of proliferation of antibody-forming cells but also the antibody-producing capacity of each cell.

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Cytodynamics of the Immune Response in Two Lines of Mice Genetically Selected for "High" and "Low" Antibody Synthesis

Cited by 98 publications

References 37 publications

Analytic Study of the Differential Anticomplementary Effects of Dextran Sulphate and Heparin in the Assay for the Mouse Alternative Pathway

Analytic Study of the Differential Anticomplementary Effects of Dextran Sulphate and Heparin in the Assay for the Mouse Alternative Pathway

Gm Allotype Genes and Gene Dosage Affecting Both IgG Subclass and IgE Levels in Atopic Patients

Immune Response against Hamster Erythrocytes in the Low‐Responder Mouse Strains

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