Cytocompatibility studies of mouse pancreatic islets on gelatin - PVP semi IPN scaffolds in vitro: Potential implication towards pancreatic tissue engineering

Muthyala, Sudhakar; Bhonde, Ramesh; Nair, Prabha D.

doi:10.4161/isl.2.6.13765

Cited by 29 publications

(16 citation statements)

References 25 publications

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“…Overall, the mechanical strength of the collagen-chitosan hydrogel was greater than collagen-only. The range of elastic moduli for both matrix formulations (hydrated) was similar to those reported for a poly(ethylene glycol) hydrogel developed for the encapsulation and rapid recovery of viable and functional 3D β-cell spheroids [45], and a gelatin-poly (vinylpyrrolidone) hydrogel which supported mouse islets for up to 30d [46]. Therefore, it appears that our matrices, and in particular the collagen-chitosan hydrogels, have suitable physical properties for the support of angiogenic cells and islets.…”

Section: Discussionsupporting

confidence: 56%

Evaluation of a Collagen-Chitosan Hydrogel for Potential Use as a Pro-Angiogenic Site for Islet Transplantation

et al. 2013

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Islet transplantation to treat type 1 diabetes (T1D) has shown varied long-term success, due in part to insufficient blood supply to maintain the islets. In the current study, collagen and collagen:chitosan (10:1) hydrogels, +/- circulating angiogenic cells (CACs), were compared for their ability to produce a pro-angiogenic environment in a streptozotocin-induced mouse model of T1D. Initial characterization showed that collagen-chitosan gels were mechanically stronger than the collagen gels (0.7kPa vs. 0.4kPa elastic modulus, respectively), had more cross-links (9.2 vs. 7.4/µm2), and were degraded more slowly by collagenase. After gelation with CACs, live/dead staining showed greater CAC viability in the collagen-chitosan gels after 18h compared to collagen (79% vs. 69%). In vivo, collagen-chitosan gels, subcutaneously implanted for up to 6 weeks in a T1D mouse, showed increased levels of pro-angiogenic cytokines over time. By 6 weeks, anti-islet cytokine levels were decreased in all matrix formulations ± CACs. The 6-week implants demonstrated increased expression of VCAM-1 in collagen-chitosan implants. Despite this, infiltrating vWF+ and CXCR4+ angiogenic cell numbers were not different between the implant types, which may be due to a delayed and reduced cytokine response in a T1D versus non-diabetic setting. The mechanical, degradation and cytokine data all suggest that the collagen-chitosan gel may be a suitable candidate for use as a pro-angiogenic ectopic islet transplant site.

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Section: Discussionsupporting

confidence: 56%

Evaluation of a Collagen-Chitosan Hydrogel for Potential Use as a Pro-Angiogenic Site for Islet Transplantation

et al. 2013

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“…Additionally, glucose is a potent stimulator for islet neogenesis and amino acids are also beneficial to the cell, which adds on the benefit of DEXGEL scaffold. Gelatin-based scaffold has been exploited in islet cell culture 35 in regard that it is a partially degradable form of collagen, which constitutes the major part of ECM in native islets. The short peptide fragments or R-G-D (Arginine-Glycine-Aspartate) sequence in gelatin plays a major role in binding of integrins, mainly a5b1 and a5b3, which helps native islets in maintaining its intact architecture.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Survival and Function of Islet-Like Clusters Differentiated from Adipose Stem Cells on a Three-Dimensional Natural Polymeric Scaffold: AnIn VitroStudy

AloysiousNeena

NairPrabha

2014

Tissue Engineering Part A

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Autologous adipose stem cells owing to its pluripotent nature offer a valuable source for pancreatic beta cell replacement in the treatment of diabetes mellitus. However, maintaining longevity and functionality of stem cell-derived islet-like cells for long-term in vitro culture is challenging. Signaling interaction between islets and surrounding extracellular matrix (ECM) is an important factor for islet survival and function. Tissue engineering strategy to use scaffolds as substitute for ECM is a key to the problem. In the present study, we fabricated a three-dimensional (3D) biodegradable scaffold comprised of natural polymers dextran and gelatin (DEXGEL) for differentiation of adipose stem cells to islet-like clusters (ILCs). Adipose stem cells derived from subcutaneous fat of New Zealand white rabbits were differentiated to ILCs on DEXGEL scaffold and two-dimensional (2D) culture plates via three stage protocol using cocktail of growth factors. The ILCs differentiated on DEXGEL scaffold exhibited characteristic islet morphology, and expressed islet-specific hormones (insulin, glucagon, and somatostatin). The insulin secretion in response to glucose challenge and viability of ILCs on DEXGEL scaffold were significantly higher in comparison to ILCs on 2D culture. Our results demonstrated for the first time that DEXGEL scaffold simulated an extracellular environment for effective differentiation of rabbit adipose stem cells to ILCs.

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“…Likewise, polyvinyl pyrrolidone (PVP) is a polymer as one of the constituent in a polymer blend having properties such as colloidal stabilizer and dispersant . PVP when used in composite polymer blends explicitly revealed properties such as hydrogelation nature, considered as a suitable container for biological cues, provides nanoporous structures, cytocompatibility and improved resistance to degradability in body fluids …”

Section: Introductionmentioning

confidence: 99%

Development of mechanically compliant 3D composite scaffolds for bone tissue engineering applications

Anandan¹,

Stella

Nambiraj³

et al. 2018

J Biomedical Materials Res

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Cancellous bone region of the human native bone has the potential to bear significant mechanical loads. However, due to the following parameters such as low trabecular volume, more porosity, less thickness and more interconnectivity, cancellous bone is accessible to damage in accidents or when aging. This research led to the effective fabrication and engineering of cancellous region of the bone for its application in reconstruction or filling of bone voids after resection of large tumor mass. Scaffolds containing hydroxyapatite (HAp), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP) (5% polymer and HAp concentrations) at different HAp: polymer composite ratios (50:50, 70:30 and 80:20), were fabricated by freeze–drying method using only water as solvent and ribose as crosslinker for the scaffolds. The fabricated scaffolds were characterized for its mechanical (compressive) strength, chemical properties using FT‐IR and XRD analyses, swelling and degradation studies, confirmation of mineralization process by immersion in simulated body fluid (SBF) for 7, 15 and 30 days, morphological analysis using scanning electron microscopy (SEM), and biocompatibility properties by performing MTT analysis. From the combined interpretation of the above mentioned tests, it was proven that 80:20 ratio of HAp to polymers was found to be the most suitable scaffold in terms of its optimal properties for its use as bone graft material for trabecular bone. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3267–3274, 2018.

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Cytocompatibility studies of mouse pancreatic islets on gelatin - PVP semi IPN scaffolds in vitro: Potential implication towards pancreatic tissue engineering

Cited by 29 publications

References 25 publications

Evaluation of a Collagen-Chitosan Hydrogel for Potential Use as a Pro-Angiogenic Site for Islet Transplantation

Evaluation of a Collagen-Chitosan Hydrogel for Potential Use as a Pro-Angiogenic Site for Islet Transplantation

Enhanced Survival and Function of Islet-Like Clusters Differentiated from Adipose Stem Cells on a Three-Dimensional Natural Polymeric Scaffold: AnIn VitroStudy

Development of mechanically compliant 3D composite scaffolds for bone tissue engineering applications

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