Cytocompatibility property evaluation of gas pressure sintered SiAlON–SiC composites with L929 fibroblast cells and Saos-2 osteoblast-like cells

Kumar, Alok; Mallik, Asok K.; Açıkbaş, Nurcan Çalış; Yaygıngöl, M.; Kara, Ferhat; Mandal, Hasan; Basu, Debabrata; Biswas, Krishanu; Basu, Bikramjit

doi:10.1016/j.msec.2011.11.019

Cited by 19 publications

(13 citation statements)

References 35 publications

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“…In research on biomaterials during last few decades, in vitro cytocompatibility has been assessed using standard cell viability/differentiation assay along with in vitro biomineralization. [28][29][30] We have carried out a detailed in vitro biomineralization study and found the formation of apatite crystals on the composite surface, indicating the good bioactivity of HA-Ti composites and this has been reported elsewhere. 28 It is important to mention here that while MTT assays provide cell viability data for various biomaterials, such assays cannot however tell whether the low viability or lower number of metabolically active cells is a result of cell death due to apoptosis or necrosis or slow proliferation of cells.…”

Section: Introductionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 57%

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Flow cytometry analysis of human fetal osteoblast fate processes on spark plasma sintered hydroxyapatite–titanium biocomposites

Kumar

Webster

Biswas

et al. 2013

J Biomedical Materials Res

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Hydroxyapatite (HA)-based biocomposites have been widely investigated for a multitude of applications and these studies have been largely driven to improve mechanical properties (toughness and strength) without compromising cytocompatibility properties. Apart from routine cell viability/proliferation analysis, limited efforts have been made to quantify the fate processes (cell proliferation, cell cycle, and cell apoptosis) of human fetal osteoblast (hFOB) cells on HA-based composites, in vitro. In this work, the osteoblast cell fate process has been studied on a model hydroxyapatite-titanium (HA-Ti) system using the flow cytometry. In order to retain both HA and Ti, the novel processing technique, that is, spark plasma sintering, was suitably adopted. The cell fate processes of hFOBs, as evaluated using a flow cytometry, revealed statistically insignificant differences among HA-10 wt % Ti and HA and control (tissue culture polystyrene surface) in terms of osteoblast apoptosis, proliferation index as well as division index. For the first time, we provide quantified flow cytometry results to demonstrate that 10 wt % Ti additions to HA do not have any significant influence on the fate processes of human osteoblast-like cells, in vitro.

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Section: Introductionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 57%

Flow cytometry analysis of human fetal osteoblast fate processes on spark plasma sintered hydroxyapatite–titanium biocomposites

Kumar

Webster

Biswas

et al. 2013

J Biomedical Materials Res

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“…We have chosen two distinct model cell lines because cell response to a material seems to be cell type dependent as reported for example by Kumar et al [41]. L929 cells from connective tissue are a cell line approved for material cytotoxicity assessment according to the ISO 10993.…”

Section: Cell Materials Interactionmentioning

confidence: 99%

Enhanced adherence of mouse fibroblast and vascular cells to plasma modified polyethylene

Řezníčková

Novotná

Kolská

et al. 2015

Materials Science and Engineering: C

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“…SiAlON ceramics are the most common materials which are used in diverse applications such as cutting tools, thermocouple protection tubes, non-ferrous molten metal handling, crucibles, wear parts, biomaterial, etc. due to high mechanical, corrosion, oxidation, wear, thermal shock resistance and high temperature properties [1][2][3][4][5][6]. However their performance sometimes is insufficient in several applications.…”

Section: Introductionmentioning

confidence: 99%

DEPENDENCE OF SiAlON-TiN COMPOSITE PROPERTIES ON TiN REINFORCEMENT PARTICLE SIZES

Açıkbaş¹,

Açıkbaş²

2018

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY a - Applied Sciences and Engineering

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In this study, the effect of TiN particle size (nano and micrometer level), being an effective parameter on microstructural evolution, mechanical and physical properties of SiAlON-TiN composites, was investigated. SEM and the XRD characterization methods were used to observe the effects of TiN particle size on the phase and microstructural evolution. Indentation fracture toughness and Vickers hardness of the manufactured specimens were measured in order to evaluate the mechanical behavior. Archimedes principle was used to determine the bulk density and porosity. Correlation between TiN particle size and hardness, fracture toughness, microstructure, phase evolution was discussed. It was observed that decrease in particle size led to little improvement in fracture toughness and hardness.

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Cytocompatibility property evaluation of gas pressure sintered SiAlON–SiC composites with L929 fibroblast cells and Saos-2 osteoblast-like cells

Cited by 19 publications

References 35 publications

Flow cytometry analysis of human fetal osteoblast fate processes on spark plasma sintered hydroxyapatite–titanium biocomposites

Flow cytometry analysis of human fetal osteoblast fate processes on spark plasma sintered hydroxyapatite–titanium biocomposites

Enhanced adherence of mouse fibroblast and vascular cells to plasma modified polyethylene

DEPENDENCE OF SiAlON-TiN COMPOSITE PROPERTIES ON TiN REINFORCEMENT PARTICLE SIZES

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