Cytocompatibility of 3D printed dental materials for temporary restorations on fibroblasts

Abstract

Background Three-dimensional (3D) printing is widely used in the fabrication of dental prostheses; however, the influence of dental materials used for 3D printing on temporary restoration of fibroblasts in tissues is unclear. Thus, the influence of different dental materials on fibroblasts were investigated. Methods Digital light processing (DLP) type 3D printing was used. Specimens in the control group were fabricated by mixing liquid and powder self-curing resin restoration materials. The temporary resin materials used were Model, Castable, Clear-SG, Tray, and Temporary, and the self-curing resin materials used were Lang dental, Alike, Milky blue, TOKVSO CUREFAST, and UniFast III. Fibroblast cells were cultured on each specimen and subsequently post-treated for analysis. Morphology of the adhered cells were observed using a confocal laser scanning microscope (CLSM) and a scanning electron microscope (SEM). Results CLSM and SEM cell imaging revealed that the 3D printed material group presented better cell adhesion with well-distributed filopodia compared to that in the conventional resin material group. Cell proliferation was significantly higher in the 3D printing materials. Conclusion This indicates that using resins fabricated by 3D printing technology rather than the ones fabricated by self-curing technology is recommended for the fabrication of dental temporary restorations.

Fibroblast behavior on conventionally processed, milled, and printed occlusal device materials with different surface treatments

“…Polished printable resins other than those tested in this study have been reported to have higher proliferation rates of L929 mouse cells than those for polished conventionally processed materials. 28 Attempts have been made to reduce the cytotoxic potential of printed materials, with postpolymerizing and rinsing in ethanol or isopropyl being reported to reduce the toxic effects of the printed materials, although the effect of rinsing depended on the material. 10,12,15,16 Most manufacturers recommend rinsing and postpolymerizing of printed parts.…”

Temporary restorations in digital technology - literature review

“…The advantages include, above all, the possibility of precise printing of restorations with complex shapes, as in the case of the CAD/CAM system, the elimination of polymerization shrinkage and thermal damage to the pulp, as well as greater biocompatibility compared to traditional selfcured materials. 35,36 The materials are available P.A. Łasica i inni www.protetstomatol.pl fused filament fabrication-modelowanie ciekłym tworzywem termoplastycznym), SLS (selektywne spiekanie laserowe), SLM (selektywne spiekanie metali), JetModeling (modelowanie strumieniowe), DLP (digital light processing-utwardzanie żywicy światłem UV), SLA (stereolitografia) oraz LFS (odmiana stereolitografii o mniejszej plamce lasera).…”

“…Do zalet należy zaliczyć przede wszystkim możliwość precyzyjnego drukowania uzupełnień o skomplikowanych kształtach, podobnie jak w przypadku techniki CAD/CAM eliminację skurczu polimeryzacyjnego oraz termicznego uszkodzenia miazgi, a także większą biokompatybilność w stosunku do tradycyjnych materiałów chemoutwardzalnych. 35,36 Materiały występują w wielu odcieniach według kolornika Vita. 33 Dodatkowo Chaturvedi i wsp.…”

Cytotoxic assessment of 3D printed photoinitiated prosthodontic resins versus heat polymerized acrylic resin (In-Vitro Study)

“…Polymers are the most used materials in the 3D printing industry due to their diversity and ease of application. In dentistry, the photoinitiated resins are the most commonly used material [27], however, due to residual monomer and photoinitiator, most photosensitive resin materials are cytotoxic, affecting cell survival and physiological functions, since monomer diffusion impairs gingival cell viability [25,28,29].…”