Cytocompatibility and blood compatibility of multifunctional fibroin/collagen/heparin scaffolds

Lü, Qiang; Zhang, Shenjia; Hu, Kun; Feng, Qingling; Cao, Chuanbao; Cui, Fuzhai

doi:10.1016/j.biomaterials.2007.01.031

Cited by 94 publications

(69 citation statements)

References 33 publications

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“…Silk fibroin is a biomaterial that has been used in surgical sutures for several thousand years due to its good mechanical and biological properties, which, in addition to its low potential for inflammatory reaction, include high strength and biocompatibility (3). In addition, silk fibroin has other advantageous characteristics, including good water vapor and oxygen permeability (4,5) and blood compatibility (6). Recently, silk fibroin has been studied for use as a wound dressing and as a scaffold for bone and cartilage regeneration (7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells

Kim¹,

Choi²,

Jeong³

et al. 2010

BMB Reports

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Section: Introductionmentioning

confidence: 99%

Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells

Kim¹,

Choi²,

Jeong³

et al. 2010

BMB Reports

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“…At the same time, drugs could be imbedded in these scaffolds and released with controlled rates. [33] However, methanol was required to prepare the scaffolds to generate insolubility in water. In prior work, we also reported that soluble silk films could be transformed to water-stable silk films with altered b-sheet content through a waterannealing process.…”

mentioning

confidence: 99%

Green Process to Prepare Silk Fibroin/Gelatin Biomaterial Scaffolds

Lü

Zhang

et al. 2010

Macromolecular Bioscience

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A new all-aqueous and green process is described to form three-dimensional porous silk fibroin matrices with control of structural and morphological features. Silk-based scaffolds are prepared using lyophilization. Gelatin is added to the aqueous silk fibroin solution to change the silk fibroin conformation and silk fibroin-water interactions through adjusting the hydrophilic interactions in silk fibroin-gelatin-water systems to restrain the formation of separate sheet like structures in the material, resulting in a more homogenous structure. Water annealing is used to generate insolubility in the silk fibroin-gelatin scaffold system, thereby avoiding the use of organic solvents such as methanol to lock in the beta-sheet structure. The adjusting of the concentration of gelatin, as well as the concentration of silk fibroin, leads to control of morphological and functional properties of the scaffolds. The scaffolds were homogeneous in terms of interconnected pores, with pore sizes ranging from 100 to 600 microm, depending on the concentration of silk fibroin used in the process. At the same time, the morphology of the scaffolds changed from lamellar sheets to porous structures based on the increase in gelatin content. Compared with salt-leaching aqueous-derived scaffolds and hexafluoroisopropanol (HFIP)-derived scaffolds, these freeze-dried scaffolds had a lower content of beta-sheet, resulting in more hydrophilic features. Most of gelatin was entrapped in the silk fibroin-gelatin scaffolds, without the burst release in PBS solution. During in vitro cell culture, these silk fibroin-gelatin scaffolds had improved cell-compatibility than salt-leaching silk fibroin scaffolds. This new process provides useful silk fibroin-based scaffold systems for use in tissue engineering. Furthermore, the whole process is green, including all-aqueous, room temperature and pressure, and without the use of toxic chemicals or solvents, offering new ways to load bioactive drugs or growth factors into the process.

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“…The amide I band (1650 cm -1 ) increases obviously. The results indicate that more random/α-helix conformation exists after modification treatment [12] . The value of amide II bands (1552 cm -1 ) is higher than control group.…”

Section: Ir Spectramentioning

confidence: 92%

“…It cannot be directly applied to a biological system because of its low biomechanical stiffness and rapid biodegradation, high thrombogenic activity, calcium deposition and so on [11] . This can be achieved by chemically crosslinking and synergisming with bioactive components, which provide biomaterials with desired properties for implantation and defect repairing [12] . Collagen matrices crosslinked by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochl oride (EDC) and N-hydroxysuccinimide (NHS) are reported to be non-cytotoxic in vitro [13] , and well biocompatibility is observed in animal studies, while other crosslinking agents, such as aldehydes and epoxide, are known to induce cytotoxic reactions during degradation process both in vitro and in vivo, thus hampering endothelialization [14] .…”

Section: Introductionmentioning

confidence: 99%

Physiochemical and biological properties of modified collagen sponge from porcine skin

Guan

et al. 2009

J. Wuhan Univ. Technol.-Mat. Sci. Edit.

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The aim of the present study was to compare one-step method to EDC/NHS crosslinking (EDC/NHS group) and one-step simultaneous method to EDC/NHS crosslinking and heparin immobilization (EDC/NHS-Heparin group) in improving physiochemical and biological properties of native collagen sponge (Control group). Modified collagen sponge overcome the disadvantages of native collagen sponge. IR spectra suggest the change of the functional groups. DSC data indicate that the stability of caloric transformation in EDC/NHS group is slightly higher than that of EDC/NHS-Heparin group. The crosslinking degree, stability against enzymes, stability in morphologically and biomechanical properties of EDC/NHS-Heparin group are higher than those of EDC/NHS group, whereas, the water-binding capacity in EDC/NHS-Heparin group is lower than that of EDC/NHS group. HUVECs in EDC/NHS-Heparin group scaffold proliferate fast, migrate well and distribute uniformly. One-step simultaneous method gains the better effects in above aspects, heparinized collagen matrices increase in angiogenic potential and suit for defect repairing and tissue engineering.

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Cytocompatibility and blood compatibility of multifunctional fibroin/collagen/heparin scaffolds

Cited by 94 publications

References 33 publications

Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells

Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells

Green Process to Prepare Silk Fibroin/Gelatin Biomaterial Scaffolds

Physiochemical and biological properties of modified collagen sponge from porcine skin

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