Cytochromes P450: terpene detoxification and pheromone production in bark beetles

Blomquist, Gary J.; Tittiger, Claus; MacLean, Marina; Keeling, Christopher I.

doi:10.1016/j.cois.2020.11.010

Cited by 28 publications

(35 citation statements)

References 46 publications

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“…Insect CYPs perform a variety of important physiological functions, and evidence for their metabolic clearance of plant volatiles or host phytochemical detoxification is accumulating ( Blomquist et al, 2021 ; Vandenhole et al, 2021 ; Nauen et al, 2022 ). Insect CYPs could be categorized into CYP2, CYP3, CYP4 and mitochondrial CYP clans ( Feyereisen, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…ODEs play an integral role in insect chemoreception and they prevent the accumulation of stimulants and subsequent sensory adaptation ( Vogt and Riddiford 1981 ; Younus et al, 2014 ). Insect ODEs have been mostly known for their involvement in the metabolism of endogenous hormones and steroids, as well as exogenous xenobiotics and allelochemicals ( Younus et al, 2014 ; Blomquist et al, 2021 ). They include a few antenna-specific or antenna-enriched cytochrome P450 (CYPs), glutathione S-transferases (GSTs), carboxylesterases (CXEs), carboxyl/cholinesterases (CCEs) and UDP-glycosyltransferases (UGTs) ( Rogers et al, 1999 ; Maïbèche-Coisne et al, 2002 ; Durand et al, 2010a ; Durand et al, 2010b ; Keeling et al, 2013 ; Bozzolan et al, 2014 ; Tan et al, 2014 ; Younus et al, 2014 ; He et al, 2015 ; Li F et al, 2018 ; Blomquist et al, 2021 ; Wang et al, 2021 ; Wei et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Insect ODEs have been mostly known for their involvement in the metabolism of endogenous hormones and steroids, as well as exogenous xenobiotics and allelochemicals ( Younus et al, 2014 ; Blomquist et al, 2021 ). They include a few antenna-specific or antenna-enriched cytochrome P450 (CYPs), glutathione S-transferases (GSTs), carboxylesterases (CXEs), carboxyl/cholinesterases (CCEs) and UDP-glycosyltransferases (UGTs) ( Rogers et al, 1999 ; Maïbèche-Coisne et al, 2002 ; Durand et al, 2010a ; Durand et al, 2010b ; Keeling et al, 2013 ; Bozzolan et al, 2014 ; Tan et al, 2014 ; Younus et al, 2014 ; He et al, 2015 ; Li F et al, 2018 ; Blomquist et al, 2021 ; Wang et al, 2021 ; Wei et al, 2021 ). The olfactory-specific GSTs protect the olfactory system from harmful xenobiotics and inactivate the components of sex pheromone in the Manduca sexta and Bombyx mori ( Rogers et al, 1999 ; Tan et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

“…This CYP enzyme catalyzes the oxidation of monoterpene pine host volatiles ( Keeling et al, 2013 ). Subsequent studies in D. ponderosae document that antennae-abundant CYP6DE1 , CYP6DJ1 , CYP6BW1 and CYP6BW3 , could oxidize and remove terpenoids from antennae, as well as detoxify host terpenoids to overcome plant defenses, in which some of the terpenoid detoxification products are used as pheromones by both sexes ( Chiu et al, 2019a ; Chiu et al, 2019b ; Chiu et al, 2019c ; Blomquist et al, 2021 ). Examples include antennal CYP6DE1 catalyzes the conversion of α -pinene into trans-verbenol, an aggregation pheromone released by female D. ponderosae ; CYP6DJ1 oxidizes terpinolene and limonene to alcohols and an epoxide, CYP6BW1 and CYP6BW3 oxidize several diterpene resin acids to cope with host defenses ( Chiu et al, 2019a ; Chiu et al, 2019b ; Chiu et al, 2019c ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Antennae-abundant expression of candidate cytochrome P450 genes associated with odorant degradation in the asian citrus psyllid, Diaphorina citri

et al. 2022

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The Asian citrus psyllid, Diaphorina citri, is a notorious pest that is an efficient vector for Candidatus Liberibacter asiaticus (CLas), the causal agent of citrus huanglongbing (HLB). The olfactory system of insects is crucial for foraging and mating behavior. Antennae-abundant odorant degrading enzymes (ODEs), including cytochrome P450 (CYPs), are important in degrading redundant odorant molecules to recover the insect olfactory. In this study, to isolate the antennal CYP genes of D. citri, we generated four transcriptomes from female/male antennae and body through deep sequencing of RNA libraries. Seven DcCYP genes preferentially expressed in antennae were first identified by comparing the antennal and body transcriptomes. Phylogenetic analysis grouped four DcCYPs (DcCYP6a13, DcCYP6j1, DcCYP6k1, and DcCYP6a2) into the CYP3 class, whereas DcCYP4d2, DcCYP4c62, and DcCYP4d8 were clustered in the CYP4 clade. qRT-PCR analyses across developmental stages and tissues showed they were antennae-abundant in both genders and constantly expressed from the first instar nymph to the adult. The results presented here highlight the isolation and expression of CYP genes in D. citri antennae, providing valuable insights into their putative role in odorant degradation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antennae-abundant expression of candidate cytochrome P450 genes associated with odorant degradation in the asian citrus psyllid, Diaphorina citri

et al. 2022

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“…P450s catalyze the metabolism of important physiologic endogenous compounds, including juvenile hormones, ecdysteroids and pheromones. The P450 genes in most insects are involved in the detoxification of plant toxins and insecticides (Blomquist et al 2020;Scott 1999). According to the phylogenetic analysis of P450s, 29, 39 and 8 CYPs were clustered into CYP4, CYP6 and CYP9 subfamily with CYP4s CYP6s and CYP9s from other insect species.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome characterization and profiling related to detoxification enzyme genes in Chilo sacchariphagus (Lepidoptera Crambidae)

Liu

et al. 2022

Entomological Research

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Chilo sacchariphagus Bojeris is one of the most dangerous pests of sugarcane. The larvae damage the seedlings and stems of sugarcane and also harm sorghum, corn and other crops, which causes great economic losses to the sugar industry every year. Transcriptome sequencing and expression profile analysis of cytochrome P450 monooxygenase (CYP), carboxylesterase and glutathione S-transferase (GST) were carried out, which could provide a basis for drug resistance monitoring and drug resistance management of the pest. Unigenes of C. sacchariphagus were obtained by using the Illumina HiSeqTM 4,000 platform as 150 bp paired-end reads. A total of 173,013 unigenes were obtained after data assembly and redundancy removal. 28,330 unigenes were annotated based on multiple public databases, and the number of unigenes annotated by NR database was the largest. According to the transcriptome analysis, 214 candidate detoxification enzyme genes were identified, including 44 GSTs, 138 CYPs, and 32 CarEs. Phylogenetic analysis showed that the CYPs were mainly clustered in CYP4, CYP6, CYP9 and CYP12 subfamilies; CarEs mainly include antennal CarEs, venom CarEs and CarEs NOTUM; while the GSTs cluster mainly contains subfamilies such as delta, omega, epsilon, theta, zeta and sigma. In this study, transcriptome information of C. sacchariphagus was obtained, and genes related to detoxification were identified, which could provide data and a basis for the further study of detoxification and host plant adaptation mechanism of C. sacchariphagus.

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Comparative genomic analysis of ABC transporter genes in Tenebrio molitor and four other tenebrionid beetles (Coleoptera: Tenebrionidea)

Xiao

Yang

et al. 2022

Arch Insect Biochem Physiol

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ATP-binding cassette (ABC) transporters, one of the largest transmembrane protein families, transport a diverse number of substate across membranes. Details of their diverse physiological functions have not been established. Here, we identified 87 ABC transporter genes in the genomes of Tenebrio molitor along with those from Asbolus verrucosus (104), Hycleus cichorii (65), and Hycleus phaleratus (80).Combining these genes (336 in total) with genes reported in Tribolium castaneum (73), we analyzed the phylogeny of ABC transporter genes in all five Tenebrionids. They are assigned into eight subfamilies (ABCA-H). In comparison to other species, the ABCC subfamily in this group of beetles appears expanded. The expression profiles of the T. molitor genes at different life stages and in various tissues were also investigated using transcriptomic analysis. Most of them display developmental specific expression patterns, suggesting to us their possible roles in development. Most of them are highly expressed in detoxification-related tissues including gut and Malpighian tubule, from which we infer their roles in insecticide resistance. We detected specific or abundant expressions of many ABC transporter genes in various tissues such as salivary gland, ovary, testis,

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Cytochromes P450: terpene detoxification and pheromone production in bark beetles

Cited by 28 publications

References 46 publications

Antennae-abundant expression of candidate cytochrome P450 genes associated with odorant degradation in the asian citrus psyllid, Diaphorina citri

Antennae-abundant expression of candidate cytochrome P450 genes associated with odorant degradation in the asian citrus psyllid, Diaphorina citri

Transcriptome characterization and profiling related to detoxification enzyme genes in Chilo sacchariphagus (Lepidoptera Crambidae)

Comparative genomic analysis of ABC transporter genes in Tenebrio molitor and four other tenebrionid beetles (Coleoptera: Tenebrionidea)

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