Cytochrome P450s and Short-chain Dehydrogenases Mediate the Toxicogenomic Response of PCB52 in the Nematode Caenorhabditis elegans

Menzel, Ralph; Yeo, Hui Ling; Rienau, Stefanie; Li, Shuang; Steinberg, Christian E. W.; Stürzenbaum, Stephen R.

doi:10.1016/j.jmb.2007.04.058

Cited by 74 publications

(65 citation statements)

References 68 publications

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“…In this context, it was a remarkable finding that Raman microspectroscopy confirmed lipids as the major contents of yellow droplets in R. similis. In Caenorhabditis elegans, intestinal oil droplets-storage components for lipophilic compounds-are involved in minimizing the toxicity of polychlorinated biphenyl (29). A similar process may be indicated in R. similis by the observed formation of bulky oil droplets containing anigorufone (1).…”

Section: Discussionmentioning

confidence: 98%

Phenalenone-type phytoalexins mediate resistance of banana plants ( Musa spp.) to the burrowing nematode Radopholus similis

Hölscher

Dhakshinamoorthy

Alexandrov

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

120

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The global yield of bananas-one of the most important food crops-is severely hampered by parasites, such as nematodes, which cause yield losses up to 75%. Plant-nematode interactions of two banana cultivars differing in susceptibility to Radopholus similis were investigated by combining the conventional and spatially resolved analytical techniques 1 H NMR spectroscopy, matrixfree UV-laser desorption/ionization mass spectrometric imaging, and Raman microspectroscopy. This innovative combination of analytical techniques was applied to isolate, identify, and locate the bananaspecific type of phytoalexins, phenylphenalenones, in the R. similiscaused lesions of the plants. The striking antinematode activity of the phenylphenalenone anigorufone, its ingestion by the nematode, and its subsequent localization in lipid droplets within the nematode is reported. The importance of varying local concentrations of these specialized metabolites in infected plant tissues, their involvement in the plant's defense system, and derived strategies for improving banana resistance are highlighted.plant protection | induced plant defense | matrix-free LDI-MSI

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Section: Discussionmentioning

confidence: 98%

Phenalenone-type phytoalexins mediate resistance of banana plants ( Musa spp.) to the burrowing nematode Radopholus similis

Hölscher

Dhakshinamoorthy

Alexandrov

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

120

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“…In the event that reasonable mutants are not officially accessible, they can be created by an assortment of methodologies. These incorporate untargeted and focused on techniques, including concoction mutagenesis, transposon inclusion, and biolistic change [240][241][242][243][244].…”

Section: Pathwaysmentioning

confidence: 99%

Discovering Capacity in Novel Targets: C. Elegans as a Model Life Form in Toxicological Research

Ac¹,

Costa²,

Tec³

et al. 2017

J Clinic Toxicol

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The nematode Caenorhabditis elegans has risen as a critical creature show in different fields including neurobiology, formative science, and hereditary qualities. Qualities of this creature demonstrate that have added to its prosperity incorporate its hereditary manipulability, invariant and completely depicted formative program, all around portrayed genome, simplicity of support, short and productive life cycle, and little body estimate. These same elements have prompted to an expanding utilization of C. elegans in toxicology, both for robotic reviews and highthroughput screening approaches. We depict a portion of the exploration that has been completed in the territories of neurotoxicology, hereditary toxicology, and ecological toxicology, and additionally high-throughput tries different things with C. elegans including all inclusive screening for sub-atomic focuses of harmfulness and fast danger evaluation for new chemicals. We contend for an expanded part for C. elegans in supplementing other model frameworks in toxicological research.

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“…Gene-arrays provide one obvious way to explore these stress-responses [27][28][29][30][31][32][33]. Gene-array studies of stressresponses in budding [28] and fission [29] yeasts show that overlapping stressor-specific patterns of gene regulation are the norm -superimposed on a core stress response (CSR) involving multiple metabolic genes, which is common to most stressors.…”

Section: Experimental Approachmentioning

confidence: 99%

“…Gene-array studies can provide complete coverage of genome-wide changes in gene expression in response to specific stressors [e.g. [30][31][32][33], but are limited by expense, insensitivity to small changes, and sometimes poor reproducibility. Such considerations effectively preclude the routine testing of multiple stressors at different doses and time-points -let alone investigating varied combinations of such stressors.…”

Section: Experimental Approachmentioning

confidence: 99%

“…We are aware that the selection of transgenic stress-reporter strains available to us is limited in scope, and that other teams have developed similar strains with proven responsiveness to particular chemical stressors. Fleshing out our transgene-based SRN with genome-wide gene-array data [27][28][29][30][31][32][33] is also vital. We see our dynamic SRN model as a resource for the entire scientific community, and all we can hope to achieve after 3 years will be a first draft, to be updated and refined in the light of future findings so that its predictions become ever more accurate.…”

Section: Community Involvement In the Srn Pro-jectmentioning

confidence: 99%

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The Stress-Response Network in Animals: Proposals to Develop a Predictive Mathematical Model

Pomerai¹,

Madhamshettiwar²,

Anbalagan³

et al. 2009

TOTOXIJ

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Abstract:Increasing evidence indicates that numerous genetic pathways responding to environmental stress in animals are regulated co-ordinately as well as independently. These stress-response systems should therefore be viewed in holistic terms as a network. As such, their behaviour is susceptible to mathematical modelling using a systems biology approach. This review outlines relevant evidence and describes a newly launched project to develop just such a model using stressresponse data from multiple transgenic strains of C. elegans and D. melanogaster. We hope that our eventual model will be capable of predicting the effects of simple stressor mixtures with reasonable accuracy. To maximise the effectiveness and scope of this model, we appeal for help from colleagues to share reagents and data relevant to this project. We also present preliminary data where RNA interference has implicated the key transcription factor DAF-16 in an unexpected upregulation of cyp-34A9 reporter expression by high cadmium. STRESS RESPONSES AND MIXTURE TOXICITYIn multicellular organisms, the defensive cellular responses evoked by environmental stresses do not result from simple linear pathways, but rather from a network of interlinked pathways with multiple outputs. This makes it difficult to predict the biological effects of multiple stressors acting together, even though this is the normal situation for industrial pollution of soil or water, where several different contaminants are usually present together. There are few studies and no useful predictive models describing the molecular responses of multicellular organisms to several toxicants acting in concert. This is essentially a systems biology problem, requiring integration of complex molecular and toxicological information. Under the auspices of a Major Award from the UK-India Education and Research Initiative (UK-IERI), we intend to develop an in silico model describing the principal elements of a consensus stress response network (SRN) and its in vivo responses to single stressors, using data from two invertebrate model systems, the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster. This model will be used to predict the likely SRN responses to stressor mixtures, and such predictions will then be tested experimentally in both species so that the model can be refined accordingly. Since the SRN core pathways are highly conserved among animal taxa, general features of this model should find wider application in ecotoxi-*Address correspondence to this author at the School of Biology, Nottingham University, Nottingham NG7 2RD, UK; Tel: (0044) 115 951 3250; Fax: (0044) 115 951 3251; E-mail: david.depomerai@nottingham.ac.uk cology. The dynamic aspects of this model, and in particular its ability to integrate multiple toxicant inputs for predicting likely SRN outputs, distinguish this from a simple map of the regulatory gene-circuits comprising the SRN. THE C. ELEGANS STRESS-RESPONSE NETWORKThe free-living soil nematode, C. elegans, is particularly suitable fo...

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Cytochrome P450s and Short-chain Dehydrogenases Mediate the Toxicogenomic Response of PCB52 in the Nematode Caenorhabditis elegans

Cited by 74 publications

References 68 publications

Phenalenone-type phytoalexins mediate resistance of banana plants ( Musa spp.) to the burrowing nematode Radopholus similis

Phenalenone-type phytoalexins mediate resistance of banana plants ( Musa spp.) to the burrowing nematode Radopholus similis

Discovering Capacity in Novel Targets: C. Elegans as a Model Life Form in Toxicological Research

The Stress-Response Network in Animals: Proposals to Develop a Predictive Mathematical Model

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