Cytochrome P450 system and heme synthesis enzymes activity in flounder liver as biomarkers of marine environments pollution

Relationships Between Physiology, Tissue Contaminants, and Biomarker Responses in Atlantic Cod (Gadus morhuaL.)

“…ALA-D has predominantly been studied as a biomarker for lead exposure (Hodson et al, 1984) and used with a range of freshwater fish species, but only a few marine species, such as flounder (Bogovski et al, 1998) and gray mullet (KrajnovicOzretic & Ozretic, 1980). ALA-D activity in red blood cells from cod sampled in different years and at different locations varied within year at any single location, between years at each location, and between locations (Figure 1).…”

Oxidative stress response of European flounder (Platichthys flesus) to cadmium determined by a custom cDNA microarray

“…Studies, such as the UK National Marine Monitoring Programme, utilise a battery of population parameters, chemical assays and biomarkers in order to gain a detailed assessment of the nature, extent and effect of exposure to an organism in a given location (MPMMG, 1998). Commonly employed molecular biomarkers include indices of the cytochrome P4501A system (CYP1A) as markers of polycyclic aromatic hydrocarbon exposure (Bogovski et al, 1998), vitellogenins for exposure to xenoestrogens (Allen et al, 1999) and metallothioneins for exposure to heavy metals (George and Young, 1986). Potential of cross-talk between biomarker systems and synergistic and antagonist effects on such systems due to exposure to mixtures is difficult to investigate and thus poorly understood.…”

Changes in the cytochrome P450 system of Baltic flounder from coastal waters of the Tallinn area in 1994–99; pp. 294–302

“…The intersite differences for the values of some biomarkers between the urban (Kakumäe) and nonurban (Lohusalu) sites in the Tallinn area of the Gulf of Finland were shown in our previous study carried out in 1994 (Bogovski et al, 1998). At that time, the total levels of CYP, aminopyrine N-demethylase, ALA-S, and HEM-S activities in the flounder liver from the urban site were higher than in the nonurban site; the AHH activity was only insignificantly elevated in the urban flounder.…”

“…The levels of FACs in bile of the flounder from Pirita, Kakumäe, and Lohusalu sites were equal and very low (0.06 ± 0.12 µg kg -1 BaP equivalents) in 1996 and 1999, with no apparent intersite differences. Bile FACs levels were not assessed at these sites in 1994, but the summarized levels of PAHs measured in fish liver tissues differed significantly in the urban and rural sites being 207 ± 45 and 42 ± 25 µg kg -1 , respectively (Bogovski et al, 1998). The levels of PAHs in liver tissues as well as in stomach contents of the flounder captured from Pirita and Lohusalu in 1996 measured using the same protocol were below the detection limit (0.005 µg kg -1 ).…”

“…AHH activities, total CYP levels, and ALA-S and HEM-S activities were measured in our previous studies of flounder liver. The results indicated sufficient differences in these indices between urban and nonurban sites in the Tallinn area in 1994 (Bogovski et al, 1998) as well as differences among flounder from 6 sites along the Baltic Sea coastline from Germany to Estonia (Bogovski et al, 1999b).…”