Cytochrome P450 gene, CYP4G51, modulates hydrocarbon production in the pea aphid, Acyrthosiphon pisum

Chen, Nan; Fan, Yongliang; Bai, Yu; Li, Xiangdong; Zhang, Zhan-Feng; Liu, Tong‐Xian

doi:10.1016/j.ibmb.2016.07.006

Cited by 86 publications

(79 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The B. germanica genome contains only one CYP4G gene, and we have found that it plays a role in hydrocarbon production. Likewise, Acyrthosiphon pisum CYP4G51 and Apis mellifera CYP4G11 , the only CYP4G member in each species, have also been shown to be involved in hydrocarbon biosynthesis . We hypothesize that if only one CYP4G member is found in a given species it would at least participate in hydrocarbon biosynthesis.…”

Section: Discussionmentioning

confidence: 96%

“…We hypothesize that if only one CYP4G member is found in a given species it would at least participate in hydrocarbon biosynthesis. Unlike the B. germanica CYP4G19 that appears to be an abdominal oenocyte‐specific expression, CYP4G11 and CYP4G51 also showed highly abdomen‐independent expression . The expression of CYP4G11 in forager antennae was recently linked to the clearance of pheromonal aldehydes .…”

Section: Discussionmentioning

confidence: 99%

“…The only CYP4G member in B. germanica , CYP4G19 , was reported to be overexpressed in a pyrethroid‐resistant strain, but no role in insecticide resistance has been attributed to CYP4G19 . Several CYP4G genes have been functionally characterized to be involved in the final decarbonylation step of hydrocarbon biosynthesis by baculovirus expression system or RNAi‐based functional studies . The insect‐specific CYP4G genes are among the rare P450 members with orthologs distributed throughout the Insecta .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Involvement of integument‐rich CYP4G19 in hydrocarbon biosynthesis and cuticular penetration resistance in Blattella germanica (L.)

Chen

Pei²,

et al. 2019

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND Cuticle penetration plays an important role as a mechanism of insecticide resistance, but the underlying molecular mechanism remains poorly understood. In Blattella germanica, the cytochrome P450 gene, CYP4G19, is overexpressed in a pyrethroid‐resistant strain. Here, we investigated whether CYP4G19 is involved in the biosynthesis of hydrocarbons and further contributes to cuticular penetration resistance in B. germanica. RESULTS Compared with the susceptible strain, pyrethroid‐resistant cockroaches showed lower cuticular permeability with Eosin Y staining. Removal of epicuticular lipids, mainly nonpolar hydrocarbons, with a hexane wash intensified the cuticular permeability and decreased the resistance index of the resistant strain. CYP4G19 was predominately expressed in the abdominal integument and could be upregulated by desiccation stress or short exposure to beta‐cypermethrin. Overexpression of CYP4G19 in the resistant strain was positively correlated with a higher level of cuticular hydrocarbons (CHCs). RNAi‐mediated knockdown of CYP4G19 significantly decreased its expression and caused a reduction in CHCs. Meanwhile, CYP4G19 suppression resulted in a non‐uniform array of the lipid layer, enhanced cuticle permeability, and compromised insecticide tolerance. CONCLUSION Our findings confirm that CYP4G19 is involved in hydrocarbon production and appears to contribute to hydrocarbon‐based penetration resistance in B. germanica. This study highlights the lipid‐based penetration resistance, advancing our understanding of the molecular mechanism underlying P450‐mediated cuticular penetration resistance in insects. © 2019 Society of Chemical Industry

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Involvement of integument‐rich CYP4G19 in hydrocarbon biosynthesis and cuticular penetration resistance in Blattella germanica (L.)

Chen

Pei²,

et al. 2019

Pest Management Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…In fact, plants and insects synthesize VLC alkanes to form a wax layer and cuticular hydrocarbons, respectively, for environmental protection [27, 28]. Arabidopsis (CER1) synthesized VLC alkanes with the range of chain lengths being C27–C31 [16], and insect CYP4G family produces C23–C33 chain lengths VLC alkanes [12, 29, 30]. Through the distribution of fatty alcohol chain lengths (Additional file 1: Figure S2b), we predicted accumulation of fatty aldehydes with the even number of chain lengths, C8–C18 in our background strain, which might be unfavorable substrates for CER1 and CYP4Gs.…”

Section: Discussionmentioning

confidence: 99%

Functional screening of aldehyde decarbonylases for long-chain alkane production by Saccharomyces cerevisiae

et al. 2017

View full text Add to dashboard Cite

BackgroundLow catalytic activities of pathway enzymes are often a limitation when using microbial based chemical production. Recent studies indicated that the enzyme activity of aldehyde decarbonylase (AD) is a critical bottleneck for alkane biosynthesis in Saccharomyces cerevisiae. We therefore performed functional screening to identify efficient ADs that can improve alkane production by S. cerevisiae.ResultsA comparative study of ADs originated from a plant, insects, and cyanobacteria were conducted in S. cerevisiae. As a result, expression of aldehyde deformylating oxygenases (ADOs), which are cyanobacterial ADs, from Synechococcus elongatus and Crocosphaera watsonii converted fatty aldehydes to corresponding Cn−1 alkanes and alkenes. The CwADO showed the highest alkane titer (0.13 mg/L/OD600) and the lowest fatty alcohol production (0.55 mg/L/OD600). However, no measurable alkanes and alkenes were detected in other AD expressed yeast strains. Dynamic expression of SeADO and CwADO under GAL promoters increased alkane production to 0.20 mg/L/OD600 and no fatty alcohols, with even number chain lengths from C8 to C14, were detected in the cells.ConclusionsWe demonstrated in vivo enzyme activities of ADs by displaying profiles of alkanes and fatty alcohols in S. cerevisiae. Among the AD enzymes evaluated, cyanobacteria ADOs were found to be suitable for alkane biosynthesis in S. cerevisiae. This work will be helpful to decide an AD candidate for alkane biosynthesis in S. cerevisiae and it will provide useful information for further investigation of AD enzymes with improved activities.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-017-0683-z) contains supplementary material, which is available to authorized users.

show abstract

“…T β H dsRNA was injected as described by Barron et al (2007), Scheiner et al (2002); Zhang et al (2016) and Chen et al (2016) with modification. A glass needle, made from a glass capillary (3.5 inc 3-000-203-G/X micropipettes, Drummond Scientific, Broomall, PA, USA) by a micropipette puller (P-97 Micropipette Puller, Sutter, CA, USA; pulling program: Pull = 100, VEL = 100, and Time = 100), was used with the Nanoject II Auto-Nanoliter Injector (Drummond Scientific Company, Broomall, PA, USA) for injection.…”

Section: Methodsmentioning

confidence: 99%

Deciphering the Function of Octopaminergic Signaling on Wing Polyphenism of the Pea Aphid Acyrthosiphon pisum

et al. 2016

Self Cite

View full text Add to dashboard Cite

Aphids exhibit wing polyphenism (winged or wingless) for adaption to predictable or temporally heterogeneous environmental changes; however, the underlying mechanism is still unclear. This morphological change could be stimulated by high aphid density, which in turn could affect octopaminergic signaling in aphids. Octopamine is a neurotransmitter synthesized in insects that can modify their physiological metabolism, locomotion, and other behaviors. We designed experiments to determine whether octopamine functions in wing formation of the pea aphid, Acyrthosiphon pisum (Harris). We determined gene expression of tyramine β-hydroxylase (TβH), a key enzyme in octopamine synthesis at different developmental stages, in different body parts, and in different densities of aphids. We also used TβH RNAi, octopamine receptor agonists (octopamine and synephrine), and an antagonist (mianserin) to modify octopaminergic signaling. We found that transcription of TβH was related to aphid density, which affected the proportion of winged offspring. By manually modifying the mother's octopaminergic signaling, TβH expression was suppressed, and TβH (enzyme) activity decreased. The proportion of winged offspring was also affected. Our results showed that octopamine could be a link in the wing determination system, as well as environmental stimulation. The RNAi results showed that the decrease of TβH expression increased aphid's reproduction; however, the decrease of TβH expression declined the numbers of winged-offspring producers, but did not affect the proportion of winged nymphs produced by the winged-offspring producer. In conclusion, the decline in the proportion of winged daughters in the next generation was caused by the decline of winged nymph producers.

show abstract

Cytochrome P450 gene, CYP4G51, modulates hydrocarbon production in the pea aphid, Acyrthosiphon pisum

Cited by 86 publications

References 64 publications

Involvement of integument‐rich CYP4G19 in hydrocarbon biosynthesis and cuticular penetration resistance in Blattella germanica (L.)

Involvement of integument‐rich CYP4G19 in hydrocarbon biosynthesis and cuticular penetration resistance in Blattella germanica (L.)

Functional screening of aldehyde decarbonylases for long-chain alkane production by Saccharomyces cerevisiae

Deciphering the Function of Octopaminergic Signaling on Wing Polyphenism of the Pea Aphid Acyrthosiphon pisum

Contact Info

Product

Resources

About