Cytochrome P450 Enzyme Systems in Fungi

Brink, Hans J M van den; Gorcom, R.F.M. van; Hondel, Cees A. M. J. J. van den; Punt, Peter J.

doi:10.1006/fgbi.1997.1021

Cited by 180 publications

(130 citation statements)

References 135 publications

Supporting

Mentioning

126

Contrasting

Order By: Relevance

“…An16g05910 and An16g05920 are adjacent genes, both downregulated in our experiment. While An16g05920 codes for a putative membrane receptor of a yet unidentified signal (De Zwaan et al, 1999), An16g05910 is homologous to gene ahbB1 of A. nidulans, which is believed to be involved in cell membrane synthesis (van den Brink et al, 1998). Its deletion in A. nidulans led to an increased branched phenotype (Lin and Momany, 2004), which closely resembled the hyphal deformations that we observed for A. niger in confrontation with Collimonas ( Figure 2b).…”

Section: Upregulation Of An12g09940 and An03g00580supporting

confidence: 59%

“…The first, An12g09940, resembles the sdeA gene of A. nidulans (Wilson et al, 2004), which encodes a D9-stearic acid desaturase for the production of unsaturated fatty acids. The second, An03g00580, codes for a putative CypX-like cytochrome P-450 (van den Brink et al, 1998). The latter is a key enzyme in the biosynthesis of ergosterol, which is required for membrane permeability and fluidity.…”

Section: Cell Membranementioning

confidence: 99%

See 1 more Smart Citation

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

et al. 2011

View full text Add to dashboard Cite

Interactions between bacteria and fungi cover a wide range of incentives, mechanisms and outcomes. The genus Collimonas consists of soil bacteria that are known for their antifungal activity and ability to grow at the expense of living fungi. In non-contact confrontation assays with the fungus Aspergillus niger, Collimonas fungivorans showed accumulation of biomass concomitant with inhibition of hyphal spread. Through microarray analysis of bacterial and fungal mRNA from the confrontation arena, we gained new insights into the mechanisms underlying the fungistatic effect and mycophagous phenotype of collimonads. Collimonas responded to the fungus by activating genes for the utilization of fungal-derived compounds and for production of a putative antifungal compound. In A. niger, differentially expressed genes included those involved in lipid and cell wall metabolism and cell defense, which correlated well with the hyphal deformations that were observed microscopically. Transcriptional profiles revealed distress in both partners: downregulation of ribosomal proteins and upregulation of mobile genetic elements in the bacteria and expression of endoplasmic reticulum stress and conidia-related genes in the fungus. Both partners experienced nitrogen shortage in each other's presence. Overall, our results indicate that the Collimonas/ Aspergillus interaction is a complex interplay between trophism, antibiosis and competition for nutrients.

show abstract

Section: Upregulation Of An12g09940 and An03g00580supporting

confidence: 59%

Section: Cell Membranementioning

confidence: 99%

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Several studies have proved the strong involvement of cytochrome P450 monooxygenases in the degradation of aromatic and xenobiotic compounds present in the environment by fungi (Van Gorcom et al 1998; Črešnar and Petrič 2011). In fungi, cytochrome P450 monooxygenases occur in multiple copies as they play wide range of roles especially in detoxification and degradation (Kameshwar and Qin 2017).…”

Section: Resultsmentioning

confidence: 99%

Comparative study of genome-wide plant biomass-degrading CAZymes in white rot, brown rot and soft rot fungi

2017

View full text Add to dashboard Cite

We have conducted a genome-level comparative study of basidiomycetes wood-rotting fungi (white, brown and soft rot) to understand the total plant biomass (lignin, cellulose, hemicellulose and pectin) -degrading abilities. We have retrieved the genome-level annotations of well-known 14 white rot fungi, 15 brown rot fungi and 13 soft rot fungi. Based on the previous literature and the annotations obtained from CAZy (carbohydrate-active enzyme) database, we have separated the genome-wide CAZymes of the selected fungi into lignin-, cellulose-, hemicellulose- and pectin-degrading enzymes. Results obtained in our study reveal that white rot fungi, especially Pleurotus eryngii and Pleurotus ostreatus potentially possess high ligninolytic ability, and soft rot fungi, especially Botryosphaeria dothidea and Fusarium oxysporum sp., potentially possess high cellulolytic, hemicellulolytic and pectinolytic abilities. The total number of genes encoding for cytochrome P450 monooxygenases and metabolic processes were high in soft and white rot fungi. We have tentatively calculated the overall lignocellulolytic abilities among the selected wood-rotting fungi which suggests that white rot fungi possess higher lignin and soft rot fungi potentially possess higher cellulolytic, hemicellulolytic and pectinolytic abilities. This approach can be applied industrially to efficiently find lignocellulolytic and aromatic compound-degrading fungi based on their genomic abilities.

show abstract

“…The ecological relations of these organisms include the metabolism of different secondary compounds, a feature sustained by diversified enzymatic systems, both intra and extra cellular, capable of carrying out numerous reactions. [6][7][8] Such attributes suggest fungi as suitable organisms to perform biotransformation reactions.…”

Section: Introductionmentioning

confidence: 99%

“…7,10 Intact fungus organisms are therefore alternatively employed in biotransformation reactions. 6,11 The fungus Cochliobolus lunatus and its conidial anamorphous form Curvularia lanata are known for their capacity of hydroxylating Δ 4-5 steroids. 12 The substrate induced 11β-hydroxylation of steroids is a classical biotransformation reaction carried out by this fungus, which tolerates structure variation in substrates.…”

Section: Introductionmentioning

confidence: 99%

Biotransformation of digitoxigenin by Cochliobolus lunatus

Pádua¹,

Oliveira²,

Filho³

et al. 2007

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

A reação de biotransformação da digitoxigenina (1) por Cochliobolus lunatus foi investigada. Foram realizados experimentos com duração de 4 dias, que resultaram no isolamento de quatro produtos, cujas estruturas químicas foram elucidadas como sendo 1β-hidroxidigitoxigenina (2), 7β-hidroxidigitoxigenina (3), 8β-hidroxidigitoxigenina (4) e digitoxigenona (5). A obtenção desses produtos nas condições empregadas nunca foi anteriormente descrita. A produção da substância 4 em uma reação de biotransformação também é inédita.The biotransformation of digitoxigenin (1) by Cochliobolus lunatus was investigated. The biotransformation reaction was carried out in a 4-day process, resulting in the isolation of four products, whose structures were elucidated as 1β-hydroxydigitoxigenin (2), 7β-hydroxydigitoxigenin (3), 8β-hydroxydigitoxigenin (4) and digitoxigenone (5). The production of these derivatives under the employed conditions has never been described so far. This is also the first report on the production of compound 4 by a biotransformation reaction.Keywords: digitoxigenin, Cochliobolus lunatus, 1β-hydroxydigitoxigenin, 7β-hydroxydigitoxigenin, 8β-hydroxydigitoxigenin, digitoxigenone IntroductionEnzymes are known to possess a wide substrate tolerance by keeping their exquisite catalytic properties with respect to chemo-, regio-and enantio-selectivity, playing an important role in biotransformations. 1 Biotransformation reactions can be accomplished at room temperature and in aqueous medium, presenting itself as a milder alternative to classical chemical reactions, 2-4 being employed for the resolution of racemates and to introduce chiral centers in substrates, among other uses. 5 Fungi are eukaryotic organisms that possess enzyme systems similar to those of mammalians. They usually present highly flexible metabolism, thus accepting varied sources of carbon and nitrogen. The ecological relations of these organisms include the metabolism of different secondary compounds, a feature sustained by diversified enzymatic systems, both intra and extra cellular, capable of carrying out numerous reactions. 6-8 Such attributes suggest fungi as suitable organisms to perform biotransformation reactions.The biotransformation of steroidal compounds by fungi has been extensively evaluated, including reactions with cardiac glycosides (Pádua et al. 9 and references herein; Table 3 of the present work). Digoxin, a Digitalis cardenolide, is still the drug of choice for the treatment of congestive heart failure, acting as a selective inhibitor of the Na + ,K + ATPase enzyme. Biotransformation of cardenolides has been investigated either as a strategy to obtain new derivatives or to convert the A-type cardenolides into the corresponding C-type compounds, which have clinical relevance. 9 The main reactions obtained so far for cardenolide biotransformation were hydroxylation in different positions of the steroidal skeleton, oxidation, glycosylation, epimerization and esterification of the hydroxyl group at C-3 (see Table 3 for references). An...

show abstract

Cytochrome P450 Enzyme Systems in Fungi

Cited by 180 publications

References 135 publications

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

Comparative study of genome-wide plant biomass-degrading CAZymes in white rot, brown rot and soft rot fungi

Biotransformation of digitoxigenin by Cochliobolus lunatus

Contact Info

Product

Resources

About