Cytochrome P450-Dependent Monooxygenase Activities and Their Inducibility by Classic P450 Inducers in the Liver, Kidney, and Nasal Mucosa of Male Adult Ring-Necked Pheasants

Giorgi, Mario; Marini, Sandra; Longo, Vincenzo; Mazzaccaro, A.; Amato, Gianluca; Gervasi, Pier Giovanni

doi:10.1006/taap.2000.8994

Cited by 14 publications

(8 citation statements)

References 45 publications

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“…Specifically, tight junctions are observed in both the nasal mucosa and the olfactory epithelium. There is significant expression and activity of a series of cytochrome P450 (CYP) isoforms, including CYP1A2, 2A, 2B, 2C, 2E, and 3A 28,29. In addition, a variety of other metabolic enzyme systems, including NADPH‐cytochrome P450 reductase, epoxide hydrolase (EH), glucuronosyltransferase (UGT), and glutathione transferase (GST) have shown significant activity in the nasal cavity 30,31.…”

Section: Common Features With the Blood–brain Barriermentioning

confidence: 99%

Nasal Drug Administration: Potential for Targeted Central Nervous System Delivery

Graff

Pollack

2005

Journal of Pharmaceutical Sciences

228

112

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Section: Common Features With the Blood–brain Barriermentioning

confidence: 99%

Nasal Drug Administration: Potential for Targeted Central Nervous System Delivery

Graff

Pollack

2005

Journal of Pharmaceutical Sciences

228

112

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“…Table 5 contains a brief summary of the P450 enzymes that have been found in chickens, turkeys, bobwhite quail, and pheasants. [37][38][39][40][41][42][43][44] …”

Section: Nrsp-7 Avian Interspecies Approachmentioning

confidence: 99%

Interspecies considerations in the evaluation of human food safety for veterinary drugs

Craigmill

Cortright

2002

AAPS J

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Residues are composed of the parent drug and metabolites, and therefore interspecies comparisons must involve a consideration of comparative xenobiotic metabolism. The focus of this article will be the residue studies that are required to establish human food safety, and the interspecies pharmacokinetic differences and similarities that impact drug residues in animal-derived foods. To illustrate the factors that can complicate and assist these comparisons, 2 drugs will be examined in detail: ivermectin and fenbendazole. In addition, the activities of 2 US programs, the Food Animal Residue Avoidance Databank (FARAD) and the NRSP-7 (National Research Support Project Number 7) Minor Use Animal Drug Program will be presented, along with strategies that may be employed in the study of species differences.

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“…On a species-tospecies basis, CYP1A varies in its forms and tissue distributions. There is a resultant variability in the measured activities and induction responsiveness of the four AROD assays (Brunstrom and Halldin, 1998;Fossi et al, 1995;Giorgi et al, 2000;Helgason et al, 2010;Herve et al, 2010;Kubota et al, 2009;Walker, 1998). Therefore, all four AROD activities were explored to assess which of the dealkylase activities are relevant in each exposure scenario and in the particular DCCO species.…”

Section: Introductionmentioning

confidence: 99%

Reprint of: CYP1A protein expression and catalytic activity in double-crested cormorants experimentally exposed to Deepwater Horizon Mississippi Canyon 252 oil

Alexander

Hooper

Cacela

et al. 2017

Ecotoxicology and Environmental Safety

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Double-crested cormorants (Phalacrocorax auritus, DCCO) were orally exposed to Deepwater Horizon Mississippi Canyon 252 (DWH) oil to investigate oil-induced toxicological impacts. Livers were collected for multiple analyses including cytochrome P4501A (CYP1A) enzymatic activity and protein expression. CYP1A enzymatic activity was measured by alkoxyresorufin O-dealkylase (AROD) assays. Activities specific to the O-dealkylation of four resorufin ethers are reported: benzyloxyresorufin O-debenzylase (BROD), ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and pentoxyresorufin O-depentylase (PROD). CYP1A protein expression was measured by western blot analysis with a CYP1A1 mouse monoclonal antibody. In study 1, hepatic BROD, EROD, and PROD activities were significantly induced in DCCO orally exposed to 20ml/kg body weight (bw) oil as a single dose or daily for 5 days. Western blot analysis revealed hepatic CYP1A protein induction in both treatment groups. In study 2 (5ml/kg bw oil or 10ml/kg bw oil, 21day exposure), all four hepatic ARODs were significantly induced. Western blots showed an increase in hepatic CYP1A expression in both treatment groups with a significant induction in birds exposed to 10ml/kg oil. Significant correlations were detected among all 4 AROD activities in both studies and between CYP1A protein expression and both MROD and PROD activities in study 2. EROD activity was highest for both treatment groups in both studies while BROD activity had the greatest fold-induction. While PROD activity values were consistently low, the fold-induction was high, usually 2nd highest to BROD activity. The observed induced AROD profiles detected in the present studies suggest both CYP1A4/1A5 DCCO isoforms are being induced after MC252 oil ingestion. A review of the literature on avian CYP1A AROD activity levels and protein expression after exposure to CYP1A inducers highlights the need for species-specific studies to accurately evaluate avian exposure to oil.

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Cytochrome P450-Dependent Monooxygenase Activities and Their Inducibility by Classic P450 Inducers in the Liver, Kidney, and Nasal Mucosa of Male Adult Ring-Necked Pheasants

Cited by 14 publications

References 45 publications

Nasal Drug Administration: Potential for Targeted Central Nervous System Delivery

Nasal Drug Administration: Potential for Targeted Central Nervous System Delivery

Interspecies considerations in the evaluation of human food safety for veterinary drugs

Reprint of: CYP1A protein expression and catalytic activity in double-crested cormorants experimentally exposed to Deepwater Horizon Mississippi Canyon 252 oil

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