Cytochrome P450-Associated Insecticide Resistance and the Development of Biochemical Diagnostic Assays in Heliothis virescens

Rose, Randy L.; Barbhaiya, Latta; Roe, R. Michael; Rock, G. C.; Hodgson, Ernest

doi:10.1006/pest.1995.1018

Cited by 122 publications

(54 citation statements)

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“…Assay of O-demethylation of p-nitroanisole (PNOD) activity by CYP450s was determined according to the method of Rose et al [23]. Reactions were carried out in 96 well micro plates by monitoring p-nitrophenol formation in a final volume of 200 μl at 405 nm using p-nitroanisole (p-NA) as a substrate at 30 s intervals for 15 min at 30 °C.…”

Section: Determination Of Cytochrome P450 Monooxygenases Activity Towmentioning

confidence: 99%

Analysing resistance of different Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) strains to abamectin insecticide

Konuş¹

2014

Turk J Bioch

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Objective: Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), tomato leafminer, is an oligophagous insect. Larvae of T. absoluta can destroy especially tomato plants which lead to important yield loss in this economically valuable crop. Chemical control through insecticides has been a main method of controlling it in farming areas all over the world. However, continues application of certain registered insecticide such as abamectin might lead to resistance development in T. absoluta. The aim of this study was to monitor resistance status of abamectin insecticide and analyse resistance mechanisms of this insecticide in T. absoluta field populations from three districts of Turkey by using bioassay and biochemical methods. Methods: Bioassays and Biochemical assays. Results: Bioassay results showed that while Adana and Antalya strain of T. absoluta showed low resistance (3.03-and 2.3-fold) to abamectin insecticide, Ankara strain of T. absoluta was not resistant to abamectin (1.31-fold). Biochemical analysis displayed that CYP450-PNOD activities showed 2.55 and 1.95-fold increase compared to susceptible population in Adana and Antalya field populations, respectively. Furthermore, GST-CDNB activities showed statistically significant (p<0.05) 1.3-fold increase only in Adana population. Although EST-α-NA activities showed 3.41-fold increase only in Ankara field population, this field population did not display a significant resistancy to abamectin. Conclusion: Consequently, cytochrome P450 monooxygenase enzymes seemed to have a major role in abamectin resistance development in field populations of T. absoluta from Turkey. In addition, GSTs possibly have supportive role such as reducing oxidative stress that developed during metabolism of abamectin in resistant field populations of T. absoluta. Key Words: Abamectin, bioassay, CYP450 monooxygenases and glutathione S-transferases, Tuta absoluta. Conflict of Interest:The authors declare no conflict of interest. ÖZETAmaç: Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), domates güvesi, olifag bir böcektir. T. absoluta'nın larvaları özellikle domates bitkisine zarar verebilirler, böylece ekonomik olarak değerli bu üründe ciddi ürün kaybına neden olurlar. Tüm dünyadaki tarım alanlarında bu zararlının kontrolünde kullanılan ana yöntem insektisitler yoluyla yapılan kimyasal mücadeledir. Ancak, abamektin gibi ruhsatlı bazı ilaçların sürekli olarak kullanılması T. absoluta'da dayanıklılık gelişimine neden olabilmektedir. Bu çalışmanın amacı biyoanaliz ve biyokimyasal yöntemleri kullanarak Türkiye'nin üç farklı bölgesindeki T. absoluta tarla populasyonlarında abamektin dayanıklılığının tespiti ve bu dayanıklılığın mekanizmasını analiz etmektir. Metod: Biyoanalizler ve Biyokimyasal analizler. Bulgular: Biyoanaliz sonuçları T. absoluta'nın Adana ve Antalya türlerinde abamektin'e karşı düşük seviyede (3.03-ve 2.3-kat) dayanıklılık olduğunu gösterirken, Ankara türünde ise abamektin'e (1.31-kat) karşı dayanıklılık olmadığını gösterdi. Biyokimyasal analiz sonuçları ise CYP450...

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Section: Determination Of Cytochrome P450 Monooxygenases Activity Towmentioning

confidence: 99%

Analysing resistance of different Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) strains to abamectin insecticide

Konuş¹

2014

Turk J Bioch

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“…However, rapid and sensitive biochemical techniques have been adopted to monitor the intensity and frequency of resistance among Þeld populations of certain species (ffrench-Constant and Roush 1991). Model substrates have been used successfully to identify a number of economically important insects with enhanced hydrolytic activity as part of their resistance mechanisms (e.g., Devonshire and Moores 1982, Byrne and Devonshire 1991, Rose et al 1995.…”

mentioning

confidence: 99%

Diagnostic Assays Based on Esterase-Mediated Resistance Mechanisms in Western Corn Rootworms (Coleoptera: Chrysomelidae)

Zhou

Scharf

Parimi

et al. 2002

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“…This technique allows both qualitative and quantitative measurement of enzyme activities from individual insects and can provide an estimate of resistance frequency in Þeld populations (Rose et al 1995). For the past 10 yr, there has been an increasing tendency to conduct simple activity assays using a 96-well microplate format.…”

Section: Discussionmentioning

confidence: 99%

Characterization of General Esterases from Methyl Parathion-Resistant and -Susceptible Populations of Western Corn Rootworm (Coleoptera: Chrysomelidae)

Zhou

Scharf

Meinke

et al. 2003

Journal of Economic Entomology

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Cytochrome P450-Associated Insecticide Resistance and the Development of Biochemical Diagnostic Assays in Heliothis virescens

Cited by 122 publications

References 0 publications

Analysing resistance of different Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) strains to abamectin insecticide

Analysing resistance of different Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) strains to abamectin insecticide

Diagnostic Assays Based on Esterase-Mediated Resistance Mechanisms in Western Corn Rootworms (Coleoptera: Chrysomelidae)

Characterization of General Esterases from Methyl Parathion-Resistant and -Susceptible Populations of Western Corn Rootworm (Coleoptera: Chrysomelidae)

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