Cytochrome P450, acetylcholinesterase and gonadal histology for evaluating contaminant exposure levels in fishes from a highly eutrophic brackish ecosystem: the Orbetello Lagoon, Italy

Corsi, Ilaria; Mariottini, Michela; Sensini, Cristiana; Lancini, L.; Focardi, Silvano

doi:10.1016/s0025-326x(02)00359-4

Cited by 53 publications

(25 citation statements)

References 36 publications

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“…Both EROD activity and CYP1A mRNA expression have been used as a specific biomarker for exposure to AhR agonists in fish (Corsi et al, 2003;Quiró et al, 2007). In the present study, the increases of both EROD activity and CYP1A mRNA expression suggest the presence of AhR agonists in the Shima River and Danshui River.…”

Section: Discussionsupporting

confidence: 51%

“…Previous studies reported variable HSI results, i.e. increase (Corsi et al, 2003;de la Torre et al, 2007), no change (Barhoumi et al, 2014), and decrease (Napierska et al, 2009) in fish from contaminated sites. Increased HSI values in mosquitofish in the present study may be the result of either hypertrophy (increase in cell size) or hyperplasia (increase in cell number) (van der Oost et al, 2003).…”

Section: Discussionmentioning

confidence: 91%

“…Cytochrome P4501A (CYP1A) plays an important role in catalyzing oxidative metabolism of organic pollutants and has been used as a specific biomarker for exposure to aryl hydrocarbon receptor (AhR) agonists in fish such as PAHs and polychlorobiphenyls (PCBs) (Olivares et al, 2010;Maes et al, 2013). Both ethoxyresorufin O-deethylase activity (EROD) and CYP1A mRNA expression have been successfully used as common biomarkers for exposure to PAHs and PCBs in fish (Corsi et al, 2003;Quiró et al, 2007). Additionally, the inhibition of AChE activity is a specific biomarker for exposure to carbamate and organophosphorus pesticides (Corsi et al, 2003).…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

“…Both ethoxyresorufin O-deethylase activity (EROD) and CYP1A mRNA expression have been successfully used as common biomarkers for exposure to PAHs and PCBs in fish (Corsi et al, 2003;Quiró et al, 2007). Additionally, the inhibition of AChE activity is a specific biomarker for exposure to carbamate and organophosphorus pesticides (Corsi et al, 2003). However, although some biomarkers can be specific to particular chemical classes, they may also be affected by other chemical classes.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

“…The condition factor of each fish was calculated as CF = (W/L 3 ) × 100, where W is body weight (g) and L is total fish length (cm) (Wijeyaratne and Pathiratne, 2006). The hepatosomatic index (HSI) was calculated as HSI = (LW / W) × 100, where W is body weight (g) and LW is liver weight (g) (Corsi et al, 2003).…”

Section: Fish Morphological Analysismentioning

confidence: 99%

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Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

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Section: Contents Lists Available At Sciencedirectmentioning

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Section: Contents Lists Available At Sciencedirectmentioning

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Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

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Effects of Benzo[a]pyrene on Food Metabolism and Reproductive Endocrine and Ovarian Development in Female Scallop Chlamys farreri at Different Reproductive Stages

Song,

Gao,

Zhou

et al. 2024

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Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbons (PAHs) with the most carcinogenic effects, has multiple toxic effects on marine bivalves. In this study, we investigated the interference mechanism of B[a]P on the food metabolism (sugars, proteins and sugars), reproductive endocrine and ovarian development in female scallops (Chlamys farreri). Scallops were exposed to different concentrations of B[a]P concentrations of 0, 0.38, 3.8 and 38 μg/L throughout gonadal development. Total cholesterol (T‐CHO) and triglyceride (TG) contents in the digestive glands were increased and their synthesis genes were up‐regulated. The plasma glucose contents decreased with the inhibition of glycogen synthesis genes and the induction of glycolysis genes in the digestive gland. B[a]P showed endocrine disrupting effects on scallops, and negatively affected genes related to ovarian cell proliferation, sex differentiation and egg development and caused damage to ovarian tissue. The findings of this study supplement the information of B[a]P disruption in gonadal development of marine bivalves.

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An integrated ecotoxicological approach to assess the effects of pollutants released by unexploded chemical ordnance dumped in the southern Adriatic (Mediterranean Sea)

et al. 2006

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In order to gain preliminary knowledge about the threat to marine ecosystems due to leakage of chemical warfare agents (CWAs) and other pollutants from rusting bombshells on the seabed, a case study was conducted in a dumping area in the southern Adriatic Sea ( depth 200 - 300 m). Following electroacoustic and magnetometric surveys of the CWA dumping area, an integrated ecotoxicological approach was used. This approach was based on analysis of CWA residues and their metabolites, including arsenic, in sediment and organisms, as well as multimarker methodology including the Health Assessment Index, histological lesion analysis and enzyme assays. Two sentinel species were selected, the blackbelly rosefish [Helicolenus dactylopterus (Delaroche, 1809)] and the European conger ( Conger conger L., 1758). Sediment analysis revealed the presence of CWA degradation products, including 1-4-thioxane and 1-4-dithiane. Tissues of fish from the CWA dumping site showed higher levels of arsenic than those from the reference site. Neither CWAs nor their metabolites were detected in fish tissues. Arsenic levels recorded in blackbelly rosefish were well above those reported for other fish species from the southern Adriatic, much higher than the FDA limit for food (2.6 mg kg(-1)) and close to the LD50 calculated for mammals ( 20 mg kg(-1) body weight). The presence of pollutants in the CWA dumping site was also confirmed by pathological lesions in both species and EROD activity, two to three times higher than in fish from the reference site (16.45 +/- 8.08 and 8.05 +/- 5.87 pmol min(-1) mg protein(-1) in blackbelly rosefish and 269 +/- 24.92 and 78.71 pmol min(-1) mg protein(-1) in European conger, respectively). Cholinesterase activity seemed unaffected in muscle of both species, whereas in brain they were one-third of those recorded in fish from the reference site (14.22 +/- 10.05 and 72.87 nmol min(-1) mg protein(-1), respectively). This suggests that acetylcholinesterase is sensitive to CWAs. In conclusion, the agreement of all the chemical and biological parameters investigated suggests that the integrated ecotoxicological approach used is appropriate to reveal the presence and biological effects of CWAs in the marine ecosystem

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Cytochrome P450, acetylcholinesterase and gonadal histology for evaluating contaminant exposure levels in fishes from a highly eutrophic brackish ecosystem: the Orbetello Lagoon, Italy

Cited by 53 publications

References 36 publications

Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

Multi-biomarker responses as indication of contaminant effects in Gambusia affinis from impacted rivers by municipal effluents

Effects of Benzo[a]pyrene on Food Metabolism and Reproductive Endocrine and Ovarian Development in Female Scallop Chlamys farreri at Different Reproductive Stages

An integrated ecotoxicological approach to assess the effects of pollutants released by unexploded chemical ordnance dumped in the southern Adriatic (Mediterranean Sea)

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