Cytochrome P-450 monooxygenase activity in herbicide-resistant and -susceptible late watergrass (Echinochloa phyllopogon)

Yun, Min-Soo; Yogo, Yasuhiro; Miura, Reiichi; Yamasue, Yuji; Fischer, Albert J.

doi:10.1016/j.pestbp.2005.04.002

Cited by 103 publications

(101 citation statements)

References 30 publications

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“…In quinclorac resistance, insensitivity of ethylene synthesis pathways that usually respond to quinclorac and enhanced activity of b-cyanoalanine synthase were suggested (Yasuor et al, 2012). In bispyribac-sodium resistance, bispyribac-sodium-induced P450s appear involved (Yun et al, 2005), and slightly increased expression of two P450 genes (not CYP81A21 or CYP81A21) has been reported under bispyribacsodium stress (Iwakami et al, 2014a).…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

et al. 2014

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Previous studies have demonstrated multiple herbicide resistance in California populations of Echinochloa phyllopogon, a noxious weed in rice (Oryza sativa) fields. It was suggested that the resistance to two classes of acetolactate synthase-inhibiting herbicides, bensulfuron-methyl (BSM) and penoxsulam (PX), may be caused by enhanced activities of herbicide-metabolizing cytochrome P450. We investigated BSM metabolism in the resistant (R) and susceptible (S) lines of E. phyllopogon, which were originally collected from different areas in California. R plants metabolized BSM through O-demethylation more rapidly than S plants. Based on available information about BSM tolerance in rice, we isolated and analyzed P450 genes of the CYP81A subfamily in E. phyllopogon. Two genes, CYP81A12 and CYP81A21, were more actively transcribed in R plants compared with S plants. Transgenic Arabidopsis (Arabidopsis thaliana) expressing either of the two genes survived in media containing BSM or PX at levels at which the wild type stopped growing. Segregation of resistances in the F2 generation from crosses of R and S plants suggested that the resistance to BSM and PX were each under the control of a single regulatory element. In F6 recombinant inbred lines, BSM and PX resistances cosegregated with increased transcript levels of CYP81A12 and CYP81A21. Heterologously produced CYP81A12 and CYP81A21 proteins in yeast (Saccharomyces cerevisiae) metabolized BSM through O-demethylation. Our results suggest that overexpression of the two P450 genes confers resistance to two classes of acetolactate synthase inhibitors to E. phyllopogon. The overexpression of the two genes could be regulated simultaneously by a single trans-acting element in the R line of E. phyllopogon.

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Section: Discussionmentioning

confidence: 99%

Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

et al. 2014

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“…It is highly selective, post-emergence low mammalian toxic and low dose (15-40 g ha -1 ) requiring herbicides so become popular now a days to weed control in rice growing area either transplanting or direct seeded (Das, 2008). Bispyribac-sodium is a pyrimidinyl carboxy herbicide, effectively controls many annual and perennial grasses, sedges and broad-leaved weeds in rice fields (Schmidt et al, 1999 andYun et al, 2005). It is selective, systemic post-emergence herbicide, with ALS (acetolactate synthase) inhibition as its mode of action and absorbed by foliage and roots.…”

Section: 6-bis [(46-dimethoxy-2-pyrimidinyl)oxy] Benzoatementioning

confidence: 99%

Integrated Weed Management in Transplanted Rice –An Effective Approach

Yadav¹,

Singh²,

Kabdal³

2018

Int.J.Curr.Microbiol.App.Sci

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“…P450s are reported to play a primary role in phase I herbicide metabolism in weeds and crops (Werck-Reichhart et al, 2000;Siminszky, 2006), and has often been detected using specific P450 inhibitors, such as the organophosphate insecticide malathion, 1-aminobenzotriazole and piperonyl butoxide (PBO) (Werck-Reichhart et al, 2000). There were reports revealed that P450 inhibitors could enhance the rate of metabolism of several herbicides and had been confirmed in some herbicide-resistant weeds (Preston, 2004;Yun et al, 2005;Yasuor et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes

Zhang

Zhao

et al. 2017

Chilean J. Agric. Res.

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American slough grass (Beckmannia syzigachne [Steud.] Fernald) is a worldwide weed and is widely distributed in rice-wheat rotations in China. Fenoxaprop-P-ethyl and mesosulfuron-methyl are two major herbicides used to control B. syzigachne. Resistance has evolved in B. syzigachne under continuous selective pressure from herbicides. This study aimed to establish the crossresistance pattern of a resistant population and explore the potential non-target-site based resistance mechanisms of B. syzigachne. Sequencing of target enzyme genes (acetyl coenzyme A carboxylase [ACCase] and acetolactate synthase [ALS]) revealed that there were no resistanceendowing amino acid substitutions in the resistant B. syzigachne population (R F1 ) compared with the sensitive population (S F1 ), and obtained the purified materials. Furthermore, piperonyl butoxide (PBO) and malathion showed synergistic effects with fenoxaprop-P-ethyl and mesosulfuron-methyl respectively in R F1 . Therefore, we speculate that the resistance observed in B. syzigachne was related with metabolic, mostly involving the cytochrome P450 enzymes. Cross resistance patterns showed that the purified resistant B. syzigachne produce high resistance to fenoxaprop-P-ethyl and pyroxsulam; intermediate resistance to flucarbazone-sodium; low resistance to quizalofop-P-ethyl, clodinafop-propargyl, sethoxydim and mesosulfuron-methyl; sensitive to clethodim pinoxaden and isoproturon.

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Cytochrome P-450 monooxygenase activity in herbicide-resistant and -susceptible late watergrass (Echinochloa phyllopogon)

Cited by 103 publications

References 30 publications

Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

Integrated Weed Management in Transplanted Rice –An Effective Approach

Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes

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